Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 52841550

GI number: 52841550

Start: 1450911

End: 1452395

Strand: Reverse

Name: epsE [H]

Synonym: lpg1319

Alternate gene names: 52841550

Gene position: 1452395-1450911 (Counterclockwise)

Preceding gene: 52841551

Following gene: 52841548

Centisome position: 42.75

GC content: 39.46

Gene sequence:

>1485_bases
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCATTTACCTAACACACCACTGCAGGCTTTCGCTGAGATAAAAAGATTATTGCAATGCGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCCGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCAGTCAGTGAAGACTTATTGGAAAA
CCAAGACGATGCCCCAATTATCCGCTTACTAAACGCTCTGTTTACCCAAGCGATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGCAATCGAATTGATGGTGTTCTGCAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACACAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAGCGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCTCATGGCATTATTTTAGTCACTGGCCCAACAGGTTCAGGCAAGACAACATCTCTCTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACGCAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCTATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACCGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCACAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCCCATGAATTAAGACAGGAAGAA
AAGGAGTTAATGGGCCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAACCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAGGGAAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACGATGGAAAATTATTTACGACCCACCACTCCAAGCATCCGTGAAGATGGTTTTAAAAGAGTATTATGTGGC
GATACGTCACTTGCAGAAATTTTAAGAGTAACCTCTCAAAACTAA

Upstream 100 bases:

>100_bases
AACAGATGATGAAACAGTGTTGCCCCCATTAACTCAAGGCAATCTACCAGTACCTTTTAGTACTCCTCCCCAATACTCAT
CAAAAATGACCAAATAAATC

Downstream 100 bases:

>100_bases
ATTACCCATACTTTAGCAATTTTTAAACAGTCACACCACATCTTCAAGATATTAATCATTTAATTTATCAGCTTAATTAA
ACAAAGTAATTAGCCTAAGA

Product: type II protein secretion ATPase LspE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Sequences:

>Translated_494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN
>Mature_494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=484, Percent_Identity=52.4793388429752, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=42.6701570680628, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI87082188, Length=255, Percent_Identity=36.4705882352941, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55152; Mature: 55152

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVD
CCCCCCCEECCCHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
ESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
FTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRAIAPLVISRVKVMAKLDIAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
HCCCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHH
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTK
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCE
VQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRL
EEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEHHHHHHHHHHHHHHHHH
HDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEEKELMGLKPDADVSQVFEPKG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCC
CDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
CCHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEC
DTSLAEILRVTSQN
CHHHHHHHHHHCCC
>Mature Secondary Structure
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVD
CCCCCCCEECCCHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
ESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
FTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRAIAPLVISRVKVMAKLDIAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
HCCCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHH
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTK
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCE
VQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRL
EEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEHHHHHHHHHHHHHHHHH
HDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEEKELMGLKPDADVSQVFEPKG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCC
CDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
CCHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEC
DTSLAEILRVTSQN
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]