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The map label for this gene is rpoS

Identifier: 52841515

GI number: 52841515

Start: 1411962

End: 1413041

Strand: Direct

Name: rpoS

Synonym: lpg1284

Alternate gene names: 52841515

Gene position: 1411962-1413041 (Clockwise)

Preceding gene: 52841514

Following gene: 52841517

Centisome position: 41.56

GC content: 38.89

Gene sequence:

>1080_bases
TTGTTAAGAAGTAAAAAACTATTTCAAGGCATAGCTAAACATATAGGTGATACAATGCAAGATGATGAAGAGCCAATTAA
GGACAAGGAATTTAAAGAAGAGGAATGGTCTGAGCCAGATGATGAATCTCTGTTGTCAGAAGAAGATGTTGAACCTGATA
TCGAAAAAATGGAAGAAGAGCTTGATGAACTTCCAGAGTTTACTGATGATGAAACTTTTCCTTCATTCCAGCGCGGGAAA
AATATTGCCAAGGCTTTAGATGCGACTCAACTTTACTTGGGTGAAATTGGTTTTTCTCCTTTGTTAACGGCTGAAGAAGA
AATCCATTATGCGACTCTTGCTTTAAAAGGTGATATGGAAGCACGCAAGAAAATGATTGAGTCGAATTTGCGCCTGGTGG
TTAAAATAGCCAGACGTTATCTTAATAGAGGATTGCCTCTTCTTGATCTAATCGAAGAGGGTAACCTGGGTTTAATGAAG
TCTGTTGAGAAATTTGACCCCAAGCGAGGATTCCGTTTTTCAACTTATGCGACCTGGTGGATTAGGCAAACTATTGAGCG
CGCAATTATGAATCAAACGAGAACTATCCGTTTGCCTATTCATGTCGTGAAAGAACTTAATGTTTATCTCAGAGCGGCAA
GGCAATTGACGCAAAAACTGGATCATGAACCATCACCTGAAGAAATAGCAGAAATGGTGGATAAGCCTTTGGAGGATGTC
CAAAAATTATTGGGATTGAATGATAAAGTCGCTTCAGTTGATACTCCAATTGGATTTGATGAAACCAAATCTTTATTAGA
TACAATTGCTGATGAAAACAGTTTAAATCCTGCAGAGCTGCTAACCAATGAGAATTTAAGGCAGCATATAGAATCTCTTC
TGGATAAATTAACTGAGAATCAACAGCAGGTTATCGCAAGACGTTTTGGGTTAAGAGGTTTTGAAAAAGCGACACTGGAA
GACGTTGGTAAGGAAATTGATTTGACCCGAGAACGCGTTCGCCAAATTCAAGTTGAAGCGTTGAAGACACTTAGAACTTT
GTTAGAGCGGGTTGGACTAACACAAGAGGATTTGTTTTGA

Upstream 100 bases:

>100_bases
GGTGTGCATTTTGAAATTAGAAGAGCAGGGGTGCCAGTTAATCCATTGAATTATCTACAAAAAGGTTGACATATAACTCA
TAGTTATAGCAACAATAGGC

Downstream 100 bases:

>100_bases
TTTAACCAGTTAATTAATTACACACAAGTTGGGTCAAATTTGACCCAACTGAAACCAAATTAATTAATTACCGTCAATTT
TACATCTTAAAAAGCAGTCA

Product: stationary phase specific sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLRSKKLFQGIAKHIGDTMQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEELDELPEFTDDETFPSFQRGK
NIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMK
SVEKFDPKRGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDV
QKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLE
DVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF

Sequences:

>Translated_359_residues
MLRSKKLFQGIAKHIGDTMQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEELDELPEFTDDETFPSFQRGK
NIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMK
SVEKFDPKRGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDV
QKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLE
DVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF
>Mature_359_residues
MLRSKKLFQGIAKHIGDTMQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEELDELPEFTDDETFPSFQRGK
NIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDMEARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMK
SVEKFDPKRGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDV
QKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTENQQQVIARRFGLRGFEKATLE
DVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=276, Percent_Identity=68.4782608695652, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=48.4444444444444, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=29.7491039426523, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=27.4509803921569, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41476; Mature: 41476

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSKKLFQGIAKHIGDTMQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEE
CCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCHHHCCCCCHHHHHCCCCCCCHHHHHHHH
LDELPEFTDDETFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCHH
ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYATWW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDV
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTEN
HHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
QQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MLRSKKLFQGIAKHIGDTMQDDEEPIKDKEFKEEEWSEPDDESLLSEEDVEPDIEKMEEE
CCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCHHHCCCCCHHHHHCCCCCCCHHHHHHHH
LDELPEFTDDETFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEECCCHH
ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYATWW
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHHH
IRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKPLEDV
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLLDKLTEN
HHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
QQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVGLTQEDLF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]