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Identifier: 52841193

GI number: 52841193

Start: 1043779

End: 1045830

Strand: Direct

Name: lig

Synonym: lpg0958

Alternate gene names: 52841193

Gene position: 1043779-1045830 (Clockwise)

Preceding gene: 52841188

Following gene: 52841194

Centisome position: 30.72

GC content: 41.72

Gene sequence:

>2052_bases
CTGTTGTTTTTATTTTGGGAATCTTCATTAATGAATGATCAAGGAATTAAGGAATCGATAGAAACGCTTAAAGAGCAAAT
AAGAAAATACGATTATCACTATTATGTTTTAGATGAACCTTTGGTTCCCGACGCGGAATATGATCGATGTTTCAAGGCAT
TGCAACAGTATGAAGAGCAATATCCGCAATTTTTATCGCCAGATTCTCCTACACAGAGAGTGAGCGGTACTCCTTCAGAT
GCTTTTATGCCGGTAGCCCATAAGCAACCCATGTTGTCTTTGTCCAATGTGTTTACTACCGATGAATTAAAAGCATTCAT
TAAGCGAGCAATTGAGAAACTGGATGAACCAAATCAACAACTGGTATTTGCTTGCGAACCAAAGCTTGATGGGTTGGCTG
TTAACATGACTTATGAGGGCGGGATCTTGACTCATGCCGCAACTCGTGGCGATGGTGCTGTAGGAGAAAACATCACAGCA
AATATTAAGACTATTGCTTCAGTTCCATTAAGGCTAAGGGTTAGTAACCCTCCAAAATTGATCGAAGTGCGGGGTGAAGT
CTATATCCCCAAAGCCGATTTTGAAGCTTACAACGCAAGGGCTAGAGAGCTCGGTGAAAAAACTTTTGCTAATCCGCGAA
ATGCTGCTGCAGGCAGTTTAAGGCAATTAAATCCTGAAATTTCTGCCAGTCGTCCACTTGCTATTTATTGTTATGGTATA
GGGGCTTGTGAGGATTATAAGTTACCTAACAGTCATTTGGAGCAATTGAATTTATTAAAAGAGTTTGGATTTAGAGTGTC
TCCAGAAACGAGGAGGGCGGTTGGAGTAGAAGGCTGTTTAGATTATTACCAGTATATGTTAGCGAAACGGAATCAATTGC
CATTTGAAATCGATGGGGTTGTTTATAAGATTGACAGTATCTCCTTGCAACAGCAATTAGGTTATGTTTCTCGTGCCCCA
AGATTTGCTTGTGCCCATAAATTTCCCGCTACAGAAGAAATGACTCGTCTGATAGCCGTGGATTTCCAGGTAGGTAGAAC
GGGCGCTGTGACACCGGTTGCACGTTTGGAGCCAGTTAGTGTTGGTGGTGTTACTGTGAGTAATGCGACTTTGCATAATT
TTGATGAAATTACCCGAAAAGACATTCGTATTGGTGATACAGTTATTATTCGCCGTGCCGGTGACGTGATCCCTGAAGTG
GTTTCTGTGATTTTGGAAAAGCGTCCCGCTAATGCCAGAATGATTGAGCTTCCTAAAAATTGCCCTGTTTGTGGTTCTGA
AGTCGTAAGGGAAGCCGATGAAGCAATTGCTCGTTGTGTCGGCGGTTTATATTGTAAAGCACAATTAAAAAGGATGATGT
GGCATTTTGCTTCTCGAAAAGCAATGTATATTGAAGGACTTGGCAGTGTTTTAATTGACCAGTTAGTTGATGAGGGTATT
GTCCATCATTTGGCGGATCTTTATGAACTCGATTTGCAGACTTTAGCTAACCTGCCAAGGATGGGGGAGAAATCTGCAAA
AAACCTATTATCCGCTTTGGAAAAAAGTAAAAAAACGACTTTCAATCGCTTTCTTTATGCTTTGGGGATCAGAGAAATCG
GTGAAGCCGGCGCAAGGGTTTTAGCGGAGCACTACTGCGATGTAGAGAGCTTGAAAGCGGCAACGATTGAGGAATTAATG
ACTCTGAATGACATAGGTCCAGTAGCGGCTTCTCATATAGTCCATTTCTTTGCCCAAGCGCATAATCTTGAAGTGATTGA
CCGTCTTCTCGAGTTGGGTATTCATTGGCCAAAGCCCGAAAAAATACAGGTTAATCAGCAAAATCCATTTTTTGGTAAAA
CAGTAGTTTTAACTGGAACTCTGAGTACCATGGGGAGGGAAGAGGCAAAGGCAAAATTATTAGCCTTGGGAGCAAAAGTA
AGTGGAAGTGTGTCTTCCAAAACGGATTATGTAGTAGCAGGAAGTGAAGCCGGTTCAAAGCTGATTAAAGCGACAGAACT
GGGAGTAGCGATTATAGAGGAAGACGAGTTTTTAAAATGGGTTAATTCATGA

Upstream 100 bases:

>100_bases
GCGCCGATTACTTGGTTCCGAATAAAGAGGCTTCCAAAAAAAAGATTTTGCCTTGAATGATTAAACCACTCATATTTGAA
AGAAGTAAAAAAATGATAAA

Downstream 100 bases:

>100_bases
GTAGCTAGATCTTTGTTATTTGGTTTATGATGGGTATCAATGAATCAGGTGAAGTAGGTATTTAAACCTGTAATAATGAC
TGATTGGCACGAGGGAAGTA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MLFLFWESSLMNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSD
AFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITA
NIKTIASVPLRLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGI
GACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAP
RFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEV
VSVILEKRPANARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGI
VHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELM
TLNDIGPVAASHIVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKV
SGSVSSKTDYVVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Sequences:

>Translated_683_residues
MLFLFWESSLMNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSD
AFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITA
NIKTIASVPLRLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGI
GACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAP
RFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEV
VSVILEKRPANARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGI
VHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELM
TLNDIGPVAASHIVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKV
SGSVSSKTDYVVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS
>Mature_683_residues
MLFLFWESSLMNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQYPQFLSPDSPTQRVSGTPSD
AFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITA
NIKTIASVPLRLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGI
GACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYMLAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAP
RFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEV
VSVILEKRPANARMIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGI
VHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELM
TLNDIGPVAASHIVHFFAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKV
SGSVSSKTDYVVAGSEAGSKLIKATELGVAIIEEDEFLKWVNS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=53.6512667660209, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=526, Percent_Identity=22.6235741444867, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75904; Mature: 75904

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLFWESSLMNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQ
CEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
YPQFLSPDSPTQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQ
CHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCE
LVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKL
EEEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCE
IEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGI
EEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEEC
GACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYMLAKRNQLPFEIDGV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCE
VYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVS
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEE
VGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPANARMIELPKN
ECCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCC
CPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
VHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEHYCDVESLKAATIEELMTLNDIGPVAASHIVHFFAQAHNLEVIDRLLELGIHWPKPE
HHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
KIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDYVVAGSEAGSK
CEEECCCCCCCCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHH
LIKATELGVAIIEEDEFLKWVNS
HHHHHHCCEEEECCHHHHHHHCC
>Mature Secondary Structure
MLFLFWESSLMNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKALQQYEEQ
CEEEEECCHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
YPQFLSPDSPTQRVSGTPSDAFMPVAHKQPMLSLSNVFTTDELKAFIKRAIEKLDEPNQQ
CHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCE
LVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPLRLRVSNPPKL
EEEEECCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEECHHHHCCCCEEEEECCCCCE
IEVRGEVYIPKADFEAYNARARELGEKTFANPRNAAAGSLRQLNPEISASRPLAIYCYGI
EEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCCCCCEEEEEEEC
GACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYMLAKRNQLPFEIDGV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCE
VYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQVGRTGAVTPVARLEPVS
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHEEEEECCCCCCCCHHHCCCEE
VGGVTVSNATLHNFDEITRKDIRIGDTVIIRRAGDVIPEVVSVILEKRPANARMIELPKN
ECCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCC
CPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRKAMYIEGLGSVLIDQLVDEGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHH
VHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKKTTFNRFLYALGIREIGEAGARV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAEHYCDVESLKAATIEELMTLNDIGPVAASHIVHFFAQAHNLEVIDRLLELGIHWPKPE
HHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
KIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLLALGAKVSGSVSSKTDYVVAGSEAGSK
CEEECCCCCCCCCEEEEEECHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHH
LIKATELGVAIIEEDEFLKWVNS
HHHHHHCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA