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The map label for this gene is rluE [H]

Identifier: 52841050

GI number: 52841050

Start: 892225

End: 892893

Strand: Reverse

Name: rluE [H]

Synonym: lpg0814

Alternate gene names: 52841050

Gene position: 892893-892225 (Counterclockwise)

Preceding gene: 52841059

Following gene: 52841040

Centisome position: 26.28

GC content: 37.52

Gene sequence:

>669_bases
CTGAAGTGGTTGCTGTCAATAACCAAAAAATTTATCATTAATTTCCACAATCAACGCAATCATAAAATGCCGCAATTAAT
ACTTTTTAATAAACCTTATGGAATCCTGACTCAATTTACTGGCGAATTACCCGAACAAACTCTCTCGGCATTTATTAAAC
TTCCGGGTTTTTATGCGGCTGGACGTCTGGATAAACAAAGTGAAGGCTTACTTATCTTAACTGACGATGGAAAACTACAA
CACCGCTTAACCCATCCTAAATTTGAGAAAAAGAAACATTACTGGGTTCAAGTAGAGGGGGTCCCTACAGACAAAGATTT
ACAACCTTTAAGAAAAGGCCTGGTAATCAAAGACATTACTTTTTTACCAGCCGAAGCCAGATTGATTTCAGAGCCAAAAA
TCTGGCCACGAATTCCACCTATAAGAGAAAGGAAACACATACCAACTACCTGGCTGGAAATTATTTTAAGAGAAGGAAAA
AATCATCAAATAAGAAAAATGACCGCTGCTATTGGATTTCCTACTTTACGTTTAATTAGATACCAGATTGGTGATTGGCA
CTTAGATCAACTCCAGCCCGGGGAATATAAATTAATCAACCTTACTAACGATAAACTGAACGAGTTAACAAGGAAGAGTA
ACCCAAGACTCGAATCCAGGGCCAAATAA

Upstream 100 bases:

>100_bases
CATATTTTAATTAATTAATGCACCTCATACCTTATTGCGTGAATGACAAAATATAACAATTATTTACGCTGTTTATCGTG
CTTAAAGGCTTGATCTTGCC

Downstream 100 bases:

>100_bases
TCATACCCAAAATGGCACTTGTCACAGGCATTATCAGGTTATAATTAAAACCTAACAGAGCATCAATAAATATAATAATT
GATTGATATAGAAAACAAAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKWLLSITKKFIINFHNQRNHKMPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAAGRLDKQSEGLLILTDDGKLQ
HRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPAEARLISEPKIWPRIPPIRERKHIPTTWLEIILREGK
NHQIRKMTAAIGFPTLRLIRYQIGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK

Sequences:

>Translated_222_residues
MKWLLSITKKFIINFHNQRNHKMPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAAGRLDKQSEGLLILTDDGKLQ
HRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPAEARLISEPKIWPRIPPIRERKHIPTTWLEIILREGK
NHQIRKMTAAIGFPTLRLIRYQIGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK
>Mature_222_residues
MKWLLSITKKFIINFHNQRNHKMPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAAGRLDKQSEGLLILTDDGKLQ
HRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDITFLPAEARLISEPKIWPRIPPIRERKHIPTTWLEIILREGK
NHQIRKMTAAIGFPTLRLIRYQIGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=57.3863636363636, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=30.8988764044944, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 26063; Mature: 26063

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLLSITKKFIINFHNQRNHKMPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAA
CHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHCCCCEEC
GRLDKQSEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDIT
CCCCCCCCCEEEEECCCCEEHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEE
FLPAEARLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIR
ECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
YQIGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK
HHCCCEECCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKWLLSITKKFIINFHNQRNHKMPQLILFNKPYGILTQFTGELPEQTLSAFIKLPGFYAA
CHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHCCCCEEC
GRLDKQSEGLLILTDDGKLQHRLTHPKFEKKKHYWVQVEGVPTDKDLQPLRKGLVIKDIT
CCCCCCCCCEEEEECCCCEEHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEE
FLPAEARLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIR
ECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHH
YQIGDWHLDQLQPGEYKLINLTNDKLNELTRKSNPRLESRAK
HHCCCEECCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]