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The map label for this gene is purB [H]

Identifier: 52841037

GI number: 52841037

Start: 875315

End: 876685

Strand: Reverse

Name: purB [H]

Synonym: lpg0801

Alternate gene names: 52841037

Gene position: 876685-875315 (Counterclockwise)

Preceding gene: 52841039

Following gene: 52841036

Centisome position: 25.8

GC content: 38.51

Gene sequence:

>1371_bases
ATGACACTTACTGCATTAAACGCGATTTCACCCATAGATGGTCGTTATGTCAATAAAACCAGAGCATTAAGTCCCTATTT
TAGTGAGTTTGCATTAACCTATTATCGTTTAATGGTTGAAATCAAGTGGTTTGAATCATTAGCTGCTAACGACACTATTC
CAGAAGTACCTGCTTTGGATAACAAGGCTCGTAAATTTTTATCTGATCTTATTTCCAATTTTAATGAATCAGAAGCTGAA
AAAATAAAAGAATTTGAAAAACAAACCAATCATGACGTTAAAGCTGTAGAGTATTATCTTCAGGATAAATTTCAGGAAAA
TGAACAACTCAAATCTTGCGTTGCTTTCATACACTTTGCCTGCACTTCAGAGGACATCAATAACCTGGCTTATGCTTTAA
TGATCAAACAAGCGATAGCCCAAGTTATACAACCTACTATAGCTGAGATCATGGGAAGCATTACGCTTCTTGGAAAACAA
CATGCTGACGTTGCGATGCTCTCAAGAACGCATGGTCAACCAGCAACACCAACCACCATGGGTAAAGAATTGGTCAATTT
TGTAGCTCGCCTTAAACGACCCCAGCAACAATTGGCAGAAGTATTAATCCCTGCCAAATTTAATGGAGCTGTTGGCAATT
ACAATGCTCACGTAGCAGCCTATCCCGAAGTGGACTGGCGTAAGCATTGCGCTAATTTTGTTACATCCCTAGGTTTATCC
TTTAACGCCTATACCACTCAAATTGAACCACATGATGGCATAGCGGAAGTCTCCCAGATTATGGTGAGAATTAATAATAT
TCTTCTGGATTATACCCAAGACATATGGAGCTATATTTCTCTTGGCTACTTTAAGCAAAAAACAATTGCCGAAGAAGTAG
GCTCATCGACCATGCCGCACAAGGTAAATCCTATAGACTTTGAAAATGCCGAAGGCAATTTAGGTCTGTCTAATGCTTTA
TTTATTCATTTTGCCAACAAATTAACTCAATCACGCATGCAAAGAGACCTGTCTGATTCGACTGTCTTACGTAACTTGGG
CGTAGCCTTCTCTTACAGTTTGATTGCCTATCACTCAGTAGCCAAAGGTAATGATAAATTACAAATCAATAAATCAGCTC
TACAAAAAGATTTATCCGAAAACTGGGAAGTTTTAGCAGAGGCCATACAAACTGTCATGCGCCGCTACAATGAACCCAAT
GCCTATGAACAATTAAAAGAGCTTACACGAGGACAAATGATAGACGCTGAAAACCTTAAAAAATTTATTAAAACCCTGTC
TATTCCCGAAGAAGCAAAAGCAGAGTTAATGAAATTAACTCCAGAGACTTACACAGGTCTTGCAACCCAATTGGTCAAGG
CTTTTTCATGA

Upstream 100 bases:

>100_bases
CAAATTTACGCTAATTTTCTAGAATATTTATCGCTTTAATTCACATCTTGTCCTGCATCTAATATTATATACGCTTTAAT
ATTGGAATAAGCTACCTATC

Downstream 100 bases:

>100_bases
GTGATCAGCTATCACAACAAGGCCAATCGTTCGAATTCGATGTTCTCGTCATTGGAACAGGATTAGCCGGGTTACATTAT
TGTTTGCAGCTTTTAGCTAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAE
KIKEFEKQTNHDVKAVEYYLQDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNAL
FIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPN
AYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS

Sequences:

>Translated_456_residues
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAE
KIKEFEKQTNHDVKAVEYYLQDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNAL
FIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPN
AYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
>Mature_455_residues
TLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEK
IKEFEKQTNHDVKAVEYYLQDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQH
ADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLSF
NAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALF
IHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNA
YEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=452, Percent_Identity=56.6371681415929, Blast_Score=540, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51403; Mature: 51272

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALD
CCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQDKFQENEQLKSCVAFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQHADVAMLSRTHGQPATPTTM
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHH
GKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNAYEQLKELTRGQMIDAENLK
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALD
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQDKFQENEQLKSCVAFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQHADVAMLSRTHGQPATPTTM
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHH
GKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNAYEQLKELTRGQMIDAENLK
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA