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The map label for this gene is pcm

Identifier: 52840937

GI number: 52840937

Start: 764716

End: 765390

Strand: Reverse

Name: pcm

Synonym: lpg0700

Alternate gene names: 52840937

Gene position: 765390-764716 (Counterclockwise)

Preceding gene: 52840944

Following gene: 52840936

Centisome position: 22.53

GC content: 37.33

Gene sequence:

>675_bases
ATGATTATTAAGACGGTAGAGCAAATGAATCACAGTGCACGCATAAATATGATCAAACAACAGTTACGAACTGGTGATGT
ATTAAACGAATCCATCCTTGACTTATACGATGAGCTCTTAAGACATGAGTTTGTTCCTGAACCTTTTTCTCATTTTGCTT
ATTCAGACATGCAAATTCCTTTGGCATACGGTCAAAGGATGTTAACTCCATTGGAAGAAGGTACAATTTTACAATCCCTT
GATCTTAAAGGTCATGAAACTGTGTTGGAAGTGGGAACAGGAACCGGTTTTATGACTGCATTACTCAGTAAGTTATGTAA
AAAAGTCATCAGTATTGACTATTATTCTGAATTTACTGCCAATGCAAAACGTAAACTCGAGGAGCATAATTGTAATAATG
TTGAATTAATTACCGGAGATGCTTGTCGTGGATGGTTGGAAAGTGCGCCATACGATGTTATCGTATTCACTGGTGCCATG
GAAAAACTGACAGATACCCATAAACTACAAATTTTACCCGGTGGCAAATTGTTCGCAATCCTTGGTAAATCCCCTGTAAT
GCAAGCTTATCTTTTTCAGTTGGATCATAATGCAATCTGGACAGAATCCATGCTTTTTGAAACGGATATACCTCCTCTGG
TAGATCAATTGAAACCGAAAGAATTTGTTTTCTAG

Upstream 100 bases:

>100_bases
TCACCATGAGATATTATCAAGAAAATTTCACTTTCAAACATTAATGCTTAGACATAAGCTGAATTCTTGCTAAAATTGCT
CCAAAGTTTTAACCTATCCA

Downstream 100 bases:

>100_bases
GGAAAACTCGAATGAGAAAATCTCTGTTTTGTTGGATTTTAACTTTAGGTGTTTCAACCCATGTGTTTGCAACAGACCTA
ATGGATATCTATCAACAAGC

Product: protein-L-isoaspartate-O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MIIKTVEQMNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHFAYSDMQIPLAYGQRMLTPLEEGTILQSL
DLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTANAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAM
EKLTDTHKLQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Sequences:

>Translated_224_residues
MIIKTVEQMNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHFAYSDMQIPLAYGQRMLTPLEEGTILQSL
DLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTANAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAM
EKLTDTHKLQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
>Mature_224_residues
MIIKTVEQMNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHFAYSDMQIPLAYGQRMLTPLEEGTILQSL
DLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTANAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAM
EKLTDTHKLQILPGGKLFAILGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=172, Percent_Identity=30.2325581395349, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25442; Mature: 25442

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKTVEQMNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHFAYSDMQIP
CCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEE
LAYGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTA
HHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHH
NAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHKLQILPGGKLFAI
HHHHHHHHCCCCCEEEEECHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEECCCCEEEE
LGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
ECCCHHHHHHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC
>Mature Secondary Structure
MIIKTVEQMNHSARINMIKQQLRTGDVLNESILDLYDELLRHEFVPEPFSHFAYSDMQIP
CCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEE
LAYGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTA
HHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHH
NAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHKLQILPGGKLFAI
HHHHHHHHCCCCCEEEEECHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEECCCCEEEE
LGKSPVMQAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
ECCCHHHHHHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA