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The map label for this gene is glpT [H]

Identifier: 52787932

GI number: 52787932

Start: 4094331

End: 4095689

Strand: Direct

Name: glpT [H]

Synonym: BLi04255

Alternate gene names: 52787932

Gene position: 4094331-4095689 (Clockwise)

Preceding gene: 52787931

Following gene: 52787934

Centisome position: 96.96

GC content: 50.55

Gene sequence:

>1359_bases
ATGTTAAAACTGTTTAAGCCAGCACCGCCGATTGAGCGGCTGCCGGAAGATCAAATTGATTCGGAATATAAGAAATTCAG
ACTGCAAGTTTTTCTCGGCATCTTCATCGGTTATGCGGCATACTATTTAATTCGCAAAAATTTCTCGCTTGCCATGCCTT
ATTTGATCGAAGAGGGCTTTTCAAAGTCGGCGCTCGGCTTTGCCTTGTCCGCTCTATCCATCTCTTACGGGCTGAGCAAG
TTCGTGATGGCCACGATATCGGACCGGAGCAATCCGAGGATGTTCCTGCCCGCCGGTTTGATCCTTTCCGCTGTCATCAG
CCTCTTGATGGGGTTTGTTCCTTTTTTCACCTCATCGATCGCCATTATGTTTATCATGCTGTTCTTAAACGGCTGGTTCC
AGGGCATGGGATGGCCGCCGTCAGGGCGTGTCCTTGTCCACTGGTTCAGCGTCAGCGAAAGGGGAAATAAAACCGCCATA
TGGAATGTCGCCCACAATGTCGGCGGGGGACTGATGGCGCCGATTGCCGTTGCCGGCGTCGCCATCTTCTCAGGCATAAC
GGGATCTGCTACAGGCTATGAAGGTGTGTTTATTTTGCCGGCCTTGGTCGCCATCGCCGTTGCCGTCATTTCTTACTGGC
TGATCCGGGATACGCCGCAGTCTGTCGGACTTCCTCCAATCGAGGAATACCGCAACGATTATTCAAGCAAATCGAAGAAA
ACCTTTGAAAAGGAATTATCAACGAAAGAAATCCTTTTCAAACATGTATTAAATAATAAATGGGTGTGGGCGATCGCACT
TGCCAACATTTTCGTGTACTTCGTCCGCTACGGCGTTCTGGATTGGGCTCCAACCTATTTAAGCGAAGAAAAAGGCTTTG
ACATGAGCAAATCAAGCGTCGCTTATTTTCTGTATGAATGGGCGGGGATTCCGGGGACATTGCTGTGCGGCTGGATCTCT
GACAAATGGTTTAAAGGACGCCGCGGACCGGCAGGCTTTGTCTTTATGGCAGGTGTTTTGATCGCGGTGCTTGTGTACTG
GTTCAACCCAGCCGGCAATCCGATGATCGACATGGCATCATTGATCGCGATCGGATTCCTCATTTACGGTCCCGTCATGC
TGATCGGCCTTCAAGCGCTTGACTTTGTTCCTAAAAAAGCGGCCGGCACGGCTGCAGGATTGACCGGTCTGTTCGGTTAC
CTGGGCGGAACCTTGACGGCGAATGCGCTGATGGGTGTTATCGTCGATGCATCCGGCTGGAATGCGGGCTTTACGCTTTT
AACCGCTTCATGTGCAATCGCAGCACTCATATTTGCGATGACTTGGAATGTGCGGGGACAGGAAGTTGTGAAGCATTAA

Upstream 100 bases:

>100_bases
CTGCTCGATTGGGGAGCGACCGGAATGTTCACCAACTTTCCGGACCGGCTGCGCGATGTTTTGCGCGAAAAGAAGTAAAT
GAATAAAGAAGGGGAATACC

Downstream 100 bases:

>100_bases
CATCAAAAAAAGATACATCTCGCAAGAAGCTGAGATGTATCTTTTTTTATTTTTATGCCATTACTTTGCATATATCGTTC
GTAAATTCGACGGGGTCTTC

Product: GlpT

Products: Proton [Cytoplasm]; glycerol-3-phosphate [Cytoplasm] [C]

Alternate protein names: G-3-P transporter; G-3-P permease [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLKLFKPAPPIERLPEDQIDSEYKKFRLQVFLGIFIGYAAYYLIRKNFSLAMPYLIEEGFSKSALGFALSALSISYGLSK
FVMATISDRSNPRMFLPAGLILSAVISLLMGFVPFFTSSIAIMFIMLFLNGWFQGMGWPPSGRVLVHWFSVSERGNKTAI
WNVAHNVGGGLMAPIAVAGVAIFSGITGSATGYEGVFILPALVAIAVAVISYWLIRDTPQSVGLPPIEEYRNDYSSKSKK
TFEKELSTKEILFKHVLNNKWVWAIALANIFVYFVRYGVLDWAPTYLSEEKGFDMSKSSVAYFLYEWAGIPGTLLCGWIS
DKWFKGRRGPAGFVFMAGVLIAVLVYWFNPAGNPMIDMASLIAIGFLIYGPVMLIGLQALDFVPKKAAGTAAGLTGLFGY
LGGTLTANALMGVIVDASGWNAGFTLLTASCAIAALIFAMTWNVRGQEVVKH

Sequences:

>Translated_452_residues
MLKLFKPAPPIERLPEDQIDSEYKKFRLQVFLGIFIGYAAYYLIRKNFSLAMPYLIEEGFSKSALGFALSALSISYGLSK
FVMATISDRSNPRMFLPAGLILSAVISLLMGFVPFFTSSIAIMFIMLFLNGWFQGMGWPPSGRVLVHWFSVSERGNKTAI
WNVAHNVGGGLMAPIAVAGVAIFSGITGSATGYEGVFILPALVAIAVAVISYWLIRDTPQSVGLPPIEEYRNDYSSKSKK
TFEKELSTKEILFKHVLNNKWVWAIALANIFVYFVRYGVLDWAPTYLSEEKGFDMSKSSVAYFLYEWAGIPGTLLCGWIS
DKWFKGRRGPAGFVFMAGVLIAVLVYWFNPAGNPMIDMASLIAIGFLIYGPVMLIGLQALDFVPKKAAGTAAGLTGLFGY
LGGTLTANALMGVIVDASGWNAGFTLLTASCAIAALIFAMTWNVRGQEVVKH
>Mature_452_residues
MLKLFKPAPPIERLPEDQIDSEYKKFRLQVFLGIFIGYAAYYLIRKNFSLAMPYLIEEGFSKSALGFALSALSISYGLSK
FVMATISDRSNPRMFLPAGLILSAVISLLMGFVPFFTSSIAIMFIMLFLNGWFQGMGWPPSGRVLVHWFSVSERGNKTAI
WNVAHNVGGGLMAPIAVAGVAIFSGITGSATGYEGVFILPALVAIAVAVISYWLIRDTPQSVGLPPIEEYRNDYSSKSKK
TFEKELSTKEILFKHVLNNKWVWAIALANIFVYFVRYGVLDWAPTYLSEEKGFDMSKSSVAYFLYEWAGIPGTLLCGWIS
DKWFKGRRGPAGFVFMAGVLIAVLVYWFNPAGNPMIDMASLIAIGFLIYGPVMLIGLQALDFVPKKAAGTAAGLTGLFGY
LGGTLTANALMGVIVDASGWNAGFTLLTASCAIAALIFAMTWNVRGQEVVKH

Specific function: Responsible for glycerol-3-phosphate uptake [H]

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family [H]

Homologues:

Organism=Homo sapiens, GI256219598, Length=437, Percent_Identity=29.0617848970252, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI4503847, Length=437, Percent_Identity=29.0617848970252, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI256219483, Length=437, Percent_Identity=29.0617848970252, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI256219543, Length=459, Percent_Identity=27.4509803921569, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI256219564, Length=354, Percent_Identity=27.683615819209, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI223717983, Length=474, Percent_Identity=24.4725738396624, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI38093649, Length=474, Percent_Identity=24.4725738396624, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI46361976, Length=475, Percent_Identity=24, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788573, Length=450, Percent_Identity=59.1111111111111, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI87082310, Length=437, Percent_Identity=37.2997711670481, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI2367259, Length=461, Percent_Identity=33.6225596529284, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI2367379, Length=330, Percent_Identity=25.1515151515152, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789515, Length=440, Percent_Identity=24.0909090909091, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082320, Length=429, Percent_Identity=23.5431235431235, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789152, Length=428, Percent_Identity=23.8317757009346, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564202, Length=425, Percent_Identity=25.6470588235294, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI133930953, Length=431, Percent_Identity=23.4338747099768, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17564204, Length=362, Percent_Identity=26.2430939226519, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17542316, Length=461, Percent_Identity=23.644251626898, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI133901854, Length=447, Percent_Identity=23.2662192393736, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI133901856, Length=447, Percent_Identity=23.2662192393736, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24656861, Length=477, Percent_Identity=23.0607966457023, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24656866, Length=496, Percent_Identity=22.5806451612903, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550478, Length=496, Percent_Identity=22.5806451612903, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005267
- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49477; Mature: 49477

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS ; PS00942 GLPT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLFKPAPPIERLPEDQIDSEYKKFRLQVFLGIFIGYAAYYLIRKNFSLAMPYLIEEGF
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
SKSALGFALSALSISYGLSKFVMATISDRSNPRMFLPAGLILSAVISLLMGFVPFFTSSI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
AIMFIMLFLNGWFQGMGWPPSGRVLVHWFSVSERGNKTAIWNVAHNVGGGLMAPIAVAGV
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCHHHHHHHHHH
AIFSGITGSATGYEGVFILPALVAIAVAVISYWLIRDTPQSVGLPPIEEYRNDYSSKSKK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
TFEKELSTKEILFKHVLNNKWVWAIALANIFVYFVRYGVLDWAPTYLSEEKGFDMSKSSV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHH
AYFLYEWAGIPGTLLCGWISDKWFKGRRGPAGFVFMAGVLIAVLVYWFNPAGNPMIDMAS
HHHHHHHHCCCHHEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LIAIGFLIYGPVMLIGLQALDFVPKKAAGTAAGLTGLFGYLGGTLTANALMGVIVDASGW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHEEECCCC
NAGFTLLTASCAIAALIFAMTWNVRGQEVVKH
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MLKLFKPAPPIERLPEDQIDSEYKKFRLQVFLGIFIGYAAYYLIRKNFSLAMPYLIEEGF
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
SKSALGFALSALSISYGLSKFVMATISDRSNPRMFLPAGLILSAVISLLMGFVPFFTSSI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
AIMFIMLFLNGWFQGMGWPPSGRVLVHWFSVSERGNKTAIWNVAHNVGGGLMAPIAVAGV
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCHHHHHHHHHH
AIFSGITGSATGYEGVFILPALVAIAVAVISYWLIRDTPQSVGLPPIEEYRNDYSSKSKK
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
TFEKELSTKEILFKHVLNNKWVWAIALANIFVYFVRYGVLDWAPTYLSEEKGFDMSKSSV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHH
AYFLYEWAGIPGTLLCGWISDKWFKGRRGPAGFVFMAGVLIAVLVYWFNPAGNPMIDMAS
HHHHHHHHCCCHHEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LIAIGFLIYGPVMLIGLQALDFVPKKAAGTAAGLTGLFGYLGGTLTANALMGVIVDASGW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHEEECCCC
NAGFTLLTASCAIAALIFAMTWNVRGQEVVKH
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glycerol-3-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glycerol-3-phosphate [Periplasm] = Proton [Cytoplasm] + glycerol-3-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8012593; 9384377 [H]