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The map label for this gene is yxjA [H]

Identifier: 52787872

GI number: 52787872

Start: 4024560

End: 4025753

Strand: Direct

Name: yxjA [H]

Synonym: BLi04195

Alternate gene names: 52787872

Gene position: 4024560-4025753 (Clockwise)

Preceding gene: 52787867

Following gene: 52787883

Centisome position: 95.31

GC content: 44.97

Gene sequence:

>1194_bases
ATGTATTTTCTCATTAATATTTTAGGCATTTTCATTGTCATGTCTGTAATTTATTTTTGTTCCCCGCGAAAGAAAAGCGT
AAAATGGAGGCCGATTCTCTCCCTTTTCATCGTTGAGCTTTTGGTGACGTGGTTTATGCTTGGGACAAAAATCGGCGGAT
GGGCCATCAACCTGATCGCCTCTTTCTTTTCGTGGCTGATCGCTTGTGCAAGCGACGGCATCGCTTTTGCCTTTCCGTCC
GTGATGGGCAATGAAACCGTTGACTTTTTCTTCAGCGCCTTGCTGCCGATTATTTTTGTTGTGACATTTTTTGATATACT
GGCTTATTTCGGAATTTTAACATGGATGATTGATAAGATCGGCTGGGTAATTTCGAAAGTCTCACGCCTTCCTAAATTAG
AAAGTTTCTTTTCGATTCAAATGATGTTTCTGGGCAATACTGAAGCGCTCGCCGTCATTCGCGGGCAATTGGCTGTATTA
AAGGATAACCGCCTGCTGACGTTCGGCATTATGAGCATGAGCAGCATCAGCGGATCGATTCTCGGTTCTTATTTAACGAT
GGTTCCTGCGACATACGTATTTACCGCCATCCCGCTAAACTGCTTAAATGCGTTGATCTTGGCCAGCATTTTAAACCCGG
TGGAAGTCACTAAGGATGAAGACATTATTTACGTACCGCCAAAAGAAGAGAAAAAGGACTTTTTCTCAACGATTTCAAAC
AGCATGATGGTCGGAATGAATATGGTCATCGTCATTCTCGCCATGGTCATCGGATATGTCGCTTTAACCTCATGTTTGAA
CGGTATTTTGGGCTTGATCGTAGACGGTTTGACGATTCAAAAGATCTTTTCTTATATCTTCAGTCCGTTCGCATTCCTGC
TTGGACTGGCGCCGGGCGACGCGATGTATGTTGCACAGCTGATGGGCATGAAACTCGCCACAAACGAATTCGTCGCGATG
ATGGATTTAAAAACGCAGCTAGACGCGATTTCACCCCATACAGCCGCCGTGGCCACAACATTTTTGACGTCGTTTGCCAA
CTTCAGTACGATCGGGATGATCTATGGAACGTACAATTCCTTATTTAACGGAGAAAAATCGGCGATTATCGGAAGAAATG
TGTGGAAGCTCCTCGTCAGCGGAATAGCAGTGTCGCTGTTGAGCGCGGGGATTGTCGGGCTGTTTGTTTGGTAG

Upstream 100 bases:

>100_bases
ATCTATTTTTCATTTGCTCTTATTTTTCATGCTTTCTTTATCGGAACCCATGAAAAATAAGAGCTTTTTTATTTTCAAAA
AACATTCGGGAGTTGGCATC

Downstream 100 bases:

>100_bases
AAAAATAGATTAAAAGGGCAGGCATCTAGCCTGCCTGCTTTATTATTAAACTTGCCTATCCCAATGGCGGTGCCCGCTGA
TCGCTTCGACCAGCTTTTTG

Product: YxjA

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MYFLINILGIFIVMSVIYFCSPRKKSVKWRPILSLFIVELLVTWFMLGTKIGGWAINLIASFFSWLIACASDGIAFAFPS
VMGNETVDFFFSALLPIIFVVTFFDILAYFGILTWMIDKIGWVISKVSRLPKLESFFSIQMMFLGNTEALAVIRGQLAVL
KDNRLLTFGIMSMSSISGSILGSYLTMVPATYVFTAIPLNCLNALILASILNPVEVTKDEDIIYVPPKEEKKDFFSTISN
SMMVGMNMVIVILAMVIGYVALTSCLNGILGLIVDGLTIQKIFSYIFSPFAFLLGLAPGDAMYVAQLMGMKLATNEFVAM
MDLKTQLDAISPHTAAVATTFLTSFANFSTIGMIYGTYNSLFNGEKSAIIGRNVWKLLVSGIAVSLLSAGIVGLFVW

Sequences:

>Translated_397_residues
MYFLINILGIFIVMSVIYFCSPRKKSVKWRPILSLFIVELLVTWFMLGTKIGGWAINLIASFFSWLIACASDGIAFAFPS
VMGNETVDFFFSALLPIIFVVTFFDILAYFGILTWMIDKIGWVISKVSRLPKLESFFSIQMMFLGNTEALAVIRGQLAVL
KDNRLLTFGIMSMSSISGSILGSYLTMVPATYVFTAIPLNCLNALILASILNPVEVTKDEDIIYVPPKEEKKDFFSTISN
SMMVGMNMVIVILAMVIGYVALTSCLNGILGLIVDGLTIQKIFSYIFSPFAFLLGLAPGDAMYVAQLMGMKLATNEFVAM
MDLKTQLDAISPHTAAVATTFLTSFANFSTIGMIYGTYNSLFNGEKSAIIGRNVWKLLVSGIAVSLLSAGIVGLFVW
>Mature_397_residues
MYFLINILGIFIVMSVIYFCSPRKKSVKWRPILSLFIVELLVTWFMLGTKIGGWAINLIASFFSWLIACASDGIAFAFPS
VMGNETVDFFFSALLPIIFVVTFFDILAYFGILTWMIDKIGWVISKVSRLPKLESFFSIQMMFLGNTEALAVIRGQLAVL
KDNRLLTFGIMSMSSISGSILGSYLTMVPATYVFTAIPLNCLNALILASILNPVEVTKDEDIIYVPPKEEKKDFFSTISN
SMMVGMNMVIVILAMVIGYVALTSCLNGILGLIVDGLTIQKIFSYIFSPFAFLLGLAPGDAMYVAQLMGMKLATNEFVAM
MDLKTQLDAISPHTAAVATTFLTSFANFSTIGMIYGTYNSLFNGEKSAIIGRNVWKLLVSGIAVSLLSAGIVGLFVW

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI227116277, Length=422, Percent_Identity=27.7251184834123, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI42542381, Length=419, Percent_Identity=30.0715990453461, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI11545853, Length=417, Percent_Identity=26.8585131894484, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788737, Length=393, Percent_Identity=33.3333333333333, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788488, Length=419, Percent_Identity=27.6849642004773, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788485, Length=420, Percent_Identity=25, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17560276, Length=395, Percent_Identity=26.3291139240506, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=402, Percent_Identity=24.1293532338308, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552519, Length=390, Percent_Identity=24.3589743589744, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45552517, Length=390, Percent_Identity=24.3589743589744, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19921868, Length=390, Percent_Identity=24.3589743589744, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281360430, Length=341, Percent_Identity=23.1671554252199, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43664; Mature: 43664

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFLINILGIFIVMSVIYFCSPRKKSVKWRPILSLFIVELLVTWFMLGTKIGGWAINLIA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFFSWLIACASDGIAFAFPSVMGNETVDFFFSALLPIIFVVTFFDILAYFGILTWMIDKI
HHHHHHHHHHCCCHHEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVISKVSRLPKLESFFSIQMMFLGNTEALAVIRGQLAVLKDNRLLTFGIMSMSSISGSI
HHHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHH
LGSYLTMVPATYVFTAIPLNCLNALILASILNPVEVTKDEDIIYVPPKEEKKDFFSTISN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCCHHHHHHHHHHHH
SMMVGMNMVIVILAMVIGYVALTSCLNGILGLIVDGLTIQKIFSYIFSPFAFLLGLAPGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCH
AMYVAQLMGMKLATNEFVAMMDLKTQLDAISPHTAAVATTFLTSFANFSTIGMIYGTYNS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LFNGEKSAIIGRNVWKLLVSGIAVSLLSAGIVGLFVW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYFLINILGIFIVMSVIYFCSPRKKSVKWRPILSLFIVELLVTWFMLGTKIGGWAINLIA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFFSWLIACASDGIAFAFPSVMGNETVDFFFSALLPIIFVVTFFDILAYFGILTWMIDKI
HHHHHHHHHHCCCHHEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWVISKVSRLPKLESFFSIQMMFLGNTEALAVIRGQLAVLKDNRLLTFGIMSMSSISGSI
HHHHHHHHHCCCHHHHHHHHHEEECCCHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHH
LGSYLTMVPATYVFTAIPLNCLNALILASILNPVEVTKDEDIIYVPPKEEKKDFFSTISN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEECCCCHHHHHHHHHHHH
SMMVGMNMVIVILAMVIGYVALTSCLNGILGLIVDGLTIQKIFSYIFSPFAFLLGLAPGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCH
AMYVAQLMGMKLATNEFVAMMDLKTQLDAISPHTAAVATTFLTSFANFSTIGMIYGTYNS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LFNGEKSAIIGRNVWKLLVSGIAVSLLSAGIVGLFVW
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]