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The map label for this gene is yfiK [H]

Identifier: 52787684

GI number: 52787684

Start: 3830803

End: 3831495

Strand: Reverse

Name: yfiK [H]

Synonym: BLi04007

Alternate gene names: 52787684

Gene position: 3831495-3830803 (Counterclockwise)

Preceding gene: 52787685

Following gene: 52787683

Centisome position: 90.74

GC content: 46.32

Gene sequence:

>693_bases
TTGCATTTTGACAGAAGGGATGTGCACGGCGGAGGACCGGTCTCCGTACAAACGATGAAAATTAAAGTGATCATTGCGGA
TGACAATTCATTCATAAGAGAGGGCATGAAAATCATATTGAGCACTTATGATGAATTTGAAGTGCTGGCGACGGTGGAGG
ATGGCTTGCAGGCGGTCGAATTTTGCCGCGCCCAAGCTGTGGATATTGCACTCCTTGATGTCCGCATGCCCAACATGGAT
GGAGTGGAAGCTGCGCGGCTCATTTCAGAAACGGGCGCCAAGCCTTTTATCTTAACAACGTTTGATGACGACGAATACAT
CGTCGCTGCGATACGGAACGGCGCAAAAGGCTATTTGCTGAAAAATGCCGAACCCGAGCGAATCAGAGACGCGATTAAAA
GTGTGTTCAACGGCCATATTGTGATGCAGGATATCATCCTCGACAAGATTAAGTCCAATTTGGCGGAGCATAAAGAAGCG
GAACCTAAGATAGACGAAAGCCTGTTTACCGAACGCGAGCTCGGGATTATGGCGCTGATCGCCAGAGGGCTGTCCAATAA
AGAAATTTCGAAGGAGCTGTTTATCTCTGAAGGTACGATTGCCAATTATATTTCCTCGATTTTGGGGAAAACAGGGCTTG
AACATCGGACGCAAATTGCGATTTATTATTTAACCGGTAAAGTTAACTTTTAA

Upstream 100 bases:

>100_bases
ACTAAAAAAGCCAATCTCTGAAAAGGATTGGCTTTTTTTATTTTAACAGGGGCTGATTCATGGTAATTTAATGTTATGAA
CAGGGCGAAAGACCCGGCTT

Downstream 100 bases:

>100_bases
CCTGGGGGATCACCATGGAATTGCGGATTATGACCGGCAAACTGATTTTGTTGGGATTTATCATCTATCAATGCGTTTTT
TCGGAAAGTGACAATCTGCC

Product: YfiK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MHFDRRDVHGGGPVSVQTMKIKVIIADDNSFIREGMKIILSTYDEFEVLATVEDGLQAVEFCRAQAVDIALLDVRMPNMD
GVEAARLISETGAKPFILTTFDDDEYIVAAIRNGAKGYLLKNAEPERIRDAIKSVFNGHIVMQDIILDKIKSNLAEHKEA
EPKIDESLFTERELGIMALIARGLSNKEISKELFISEGTIANYISSILGKTGLEHRTQIAIYYLTGKVNF

Sequences:

>Translated_230_residues
MHFDRRDVHGGGPVSVQTMKIKVIIADDNSFIREGMKIILSTYDEFEVLATVEDGLQAVEFCRAQAVDIALLDVRMPNMD
GVEAARLISETGAKPFILTTFDDDEYIVAAIRNGAKGYLLKNAEPERIRDAIKSVFNGHIVMQDIILDKIKSNLAEHKEA
EPKIDESLFTERELGIMALIARGLSNKEISKELFISEGTIANYISSILGKTGLEHRTQIAIYYLTGKVNF
>Mature_230_residues
MHFDRRDVHGGGPVSVQTMKIKVIIADDNSFIREGMKIILSTYDEFEVLATVEDGLQAVEFCRAQAVDIALLDVRMPNMD
GVEAARLISETGAKPFILTTFDDDEYIVAAIRNGAKGYLLKNAEPERIRDAIKSVFNGHIVMQDIILDKIKSNLAEHKEA
EPKIDESLFTERELGIMALIARGLSNKEISKELFISEGTIANYISSILGKTGLEHRTQIAIYYLTGKVNF

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=188, Percent_Identity=35.6382978723404, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788521, Length=208, Percent_Identity=32.2115384615385, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787473, Length=202, Percent_Identity=29.7029702970297, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786747, Length=194, Percent_Identity=27.319587628866, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790102, Length=202, Percent_Identity=25.2475247524752, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788724, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25635; Mature: 25635

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFDRRDVHGGGPVSVQTMKIKVIIADDNSFIREGMKIILSTYDEFEVLATVEDGLQAVE
CCCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHH
FCRAQAVDIALLDVRMPNMDGVEAARLISETGAKPFILTTFDDDEYIVAAIRNGAKGYLL
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEEE
KNAEPERIRDAIKSVFNGHIVMQDIILDKIKSNLAEHKEAEPKIDESLFTERELGIMALI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARGLSNKEISKELFISEGTIANYISSILGKTGLEHRTQIAIYYLTGKVNF
HHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MHFDRRDVHGGGPVSVQTMKIKVIIADDNSFIREGMKIILSTYDEFEVLATVEDGLQAVE
CCCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHH
FCRAQAVDIALLDVRMPNMDGVEAARLISETGAKPFILTTFDDDEYIVAAIRNGAKGYLL
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEEE
KNAEPERIRDAIKSVFNGHIVMQDIILDKIKSNLAEHKEAEPKIDESLFTERELGIMALI
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARGLSNKEISKELFISEGTIANYISSILGKTGLEHRTQIAIYYLTGKVNF
HHCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]