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The map label for this gene is pyrG

Identifier: 52787641

GI number: 52787641

Start: 3787475

End: 3789082

Strand: Reverse

Name: pyrG

Synonym: BLi03963

Alternate gene names: 52787641

Gene position: 3789082-3787475 (Counterclockwise)

Preceding gene: 52787642

Following gene: 52787639

Centisome position: 89.73

GC content: 48.07

Gene sequence:

>1608_bases
ATGACAAAATATATCTTTGTAACCGGAGGAGTTGTATCCTCGCTTGGAAAAGGAATTACGGCGTCTTCGCTCGGCCGCCT
GCTGAAGAACAGAGGTTTGAATGTCACCATTCAAAAATTTGACCCGTATATCAACGTAGACCCGGGTACGATGAGCCCGT
ATCAGCACGGTGAAGTGTTTGTAACCGACGACGGTGCGGAAACGGATCTTGACCTCGGCCATTATGAGCGCTTTATCGAC
ATCAATTTAAATAAATACAGCAACGTGACAACAGGAAAAATCTATTCCACGGTGCTGAAAAAAGAGCGCAGAGGCGATTA
TTTAGGCGGAACCGTACAGGTAATACCTCATATCACAAATGAAATTAAAGACCGTGTATTCCGCGCCGGAAAAGAAACGC
ACGCTGATGTCGTAATCACTGAAATCGGCGGAACCGTCGGTGACATCGAATCGCTGCCATTTCTTGAAGCGATCCGCCAG
ATCAAGAGCGATGTCGGCCGCGACAATGTGATGTACATCCATTGTACGCTTGTTCCATATCTTAAAGCGGCGGGAGAAAT
GAAAACAAAGCCGACTCAGCACAGCGTAAAAGAACTGCGCAGCCTCGGCATTCAGCCAAATGTCATCGTCGTGAGAACAG
AAATGCCGATTTCTCAGGACATGAAAGATAAAATCGCGCTCTTCTGCGACATTGATCCTAAAGCCGTCATCGAGGCTGGA
GATGCGGATACGCTCTACTCCATTCCTCTCGACCTTCAAAAACAAGGCCTGGACAGCCTGGTTTGCAGCCACTTGAAGCT
GGATTGCAGAGAAGCCGATATGGAAGAGTGGAAAGAACTCGTCAAAAAGGTGAAAAATCTGTCCAAAACGGTAACAATCG
CTTTGGTCGGTAAATACGTTGAGCTCCCGGATGCTTACATTTCAGTTGTAGAATCGCTTCGCCATGCGGGTTATGCGTTT
GATGCAGACATTCAAGTTAAATGGATCAATGCGGAAGAAGTGACGGAAGACAACGTTGCCGATCTTGTTCAAAATGCGGA
CGGTATTCTTGTTCCGGGCGGATTCGGAGACCGCGGTGTTGAAGGGAAAATCACGACAGTCAAATATGCCCGCGAACAAA
AGATTCCGTTCTTCGGCATTTGCCTCGGAATGCAGGTGGCATCGATTGAATACGCGAGAAACGTGCTCGGCCTTGAAGGA
GCGCACTCAGCCGAAATCGATCCGTCTACACCGTATCCGATTATCGACCTTCTCCCTGAACAAAAGGACATTGAAGACCT
CGGCGGAACACTGCGCCTCGGACTTTATCCGTGCAAGCTTCAAGAAGGATCGAAAGCATATCAGGCATATGAAAATGAAG
TGGTGTATGAACGCCACCGCCACCGCTACGAATTCAACAATGAGTTCAGACAGCAGATGGAAGAAGCCGGTTTTGTATTT
TCCGGGACAAGCCCTGACGGCCGTCTCGTAGAAATTATCGAGCTTAAAGACCATCCATGGTTTGTCGCGTCTCAGTTCCA
TCCTGAATTTACGTCAAGACCGACAAGACCTCAGGCTCTGTTCAGAGACTTCGTACATGCTTCGCTAAAGACTTCGGAAA
AGCTGTAA

Upstream 100 bases:

>100_bases
CGCCTCCTAATCATTTAAATCATTTAGAAAAGATCTAAATGATTTTTCATAAAAAGAGCATTTTAAGAAATAAGCGTTAA
TCGAAGAGAGGGGTACAGAA

Downstream 100 bases:

>100_bases
AAAAGAAAAAGCCTGCCGGCAAATCACGCCGGCGGGCTTTTTTTGGCTCTTTCTGATGATAATGGCGGCCCGAACTGGTA
CTATATTCATTAGGCCGGCC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTKYIFVTGGVVSSLGKGITASSLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKYSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNEIKDRVFRAGKETHADVVITEIGGTVGDIESLPFLEAIRQ
IKSDVGRDNVMYIHCTLVPYLKAAGEMKTKPTQHSVKELRSLGIQPNVIVVRTEMPISQDMKDKIALFCDIDPKAVIEAG
DADTLYSIPLDLQKQGLDSLVCSHLKLDCREADMEEWKELVKKVKNLSKTVTIALVGKYVELPDAYISVVESLRHAGYAF
DADIQVKWINAEEVTEDNVADLVQNADGILVPGGFGDRGVEGKITTVKYAREQKIPFFGICLGMQVASIEYARNVLGLEG
AHSAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLQEGSKAYQAYENEVVYERHRHRYEFNNEFRQQMEEAGFVF
SGTSPDGRLVEIIELKDHPWFVASQFHPEFTSRPTRPQALFRDFVHASLKTSEKL

Sequences:

>Translated_535_residues
MTKYIFVTGGVVSSLGKGITASSLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLNKYSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNEIKDRVFRAGKETHADVVITEIGGTVGDIESLPFLEAIRQ
IKSDVGRDNVMYIHCTLVPYLKAAGEMKTKPTQHSVKELRSLGIQPNVIVVRTEMPISQDMKDKIALFCDIDPKAVIEAG
DADTLYSIPLDLQKQGLDSLVCSHLKLDCREADMEEWKELVKKVKNLSKTVTIALVGKYVELPDAYISVVESLRHAGYAF
DADIQVKWINAEEVTEDNVADLVQNADGILVPGGFGDRGVEGKITTVKYAREQKIPFFGICLGMQVASIEYARNVLGLEG
AHSAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLQEGSKAYQAYENEVVYERHRHRYEFNNEFRQQMEEAGFVF
SGTSPDGRLVEIIELKDHPWFVASQFHPEFTSRPTRPQALFRDFVHASLKTSEKL
>Mature_534_residues
TKYIFVTGGVVSSLGKGITASSLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDI
NLNKYSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITNEIKDRVFRAGKETHADVVITEIGGTVGDIESLPFLEAIRQI
KSDVGRDNVMYIHCTLVPYLKAAGEMKTKPTQHSVKELRSLGIQPNVIVVRTEMPISQDMKDKIALFCDIDPKAVIEAGD
ADTLYSIPLDLQKQGLDSLVCSHLKLDCREADMEEWKELVKKVKNLSKTVTIALVGKYVELPDAYISVVESLRHAGYAFD
ADIQVKWINAEEVTEDNVADLVQNADGILVPGGFGDRGVEGKITTVKYAREQKIPFFGICLGMQVASIEYARNVLGLEGA
HSAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLQEGSKAYQAYENEVVYERHRHRYEFNNEFRQQMEEAGFVFS
GTSPDGRLVEIIELKDHPWFVASQFHPEFTSRPTRPQALFRDFVHASLKTSEKL

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=46.3503649635037, Blast_Score=505, Evalue=1e-143,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=46.0694698354662, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=46.0694698354662, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=46.0694698354662, Blast_Score=494, Evalue=1e-140,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=56.9832402234637, Blast_Score=615, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=39.1376451077944, Blast_Score=429, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=46.6071428571429, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=45.6483126110124, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=46.7153284671533, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=44.421906693712, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_BACLD (Q65DT7)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081040.2
- RefSeq:   YP_093470.1
- HSSP:   P17812
- ProteinModelPortal:   Q65DT7
- SMR:   Q65DT7
- STRING:   Q65DT7
- EnsemblBacteria:   EBBACT00000057823
- EnsemblBacteria:   EBBACT00000061287
- GeneID:   3027730
- GeneID:   3101371
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03963
- KEGG:   bli:BL03966
- NMPDR:   fig|279010.5.peg.4100
- eggNOG:   COG0504
- GeneTree:   EBGT00050000002533
- HOGENOM:   HBG597806
- ProtClustDB:   PRK05380
- BioCyc:   BLIC279010-1:BLI03963-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59809; Mature: 59678

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGITASSLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKDRVFRAGKETHADVVITEIGGTVGDIESLPFLEAIRQIKSDVGRDNVMYIHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LKAAGEMKTKPTQHSVKELRSLGIQPNVIVVRTEMPISQDMKDKIALFCDIDPKAVIEAG
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHEEECC
DADTLYSIPLDLQKQGLDSLVCSHLKLDCREADMEEWKELVKKVKNLSKTVTIALVGKYV
CCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECHH
ELPDAYISVVESLRHAGYAFDADIQVKWINAEEVTEDNVADLVQNADGILVPGGFGDRGV
HCCHHHHHHHHHHHHCCEEECCCEEEEEECHHHCCCCHHHHHHHCCCCEEECCCCCCCCC
EGKITTVKYAREQKIPFFGICLGMQVASIEYARNVLGLEGAHSAEIDPSTPYPIIDLLPE
CCEEEEEEEHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCC
QKDIEDLGGTLRLGLYPCKLQEGSKAYQAYENEVVYERHRHRYEFNNEFRQQMEEAGFVF
CCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SGTSPDGRLVEIIELKDHPWFVASQFHPEFTSRPTRPQALFRDFVHASLKTSEKL
ECCCCCCCEEEEEEECCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGITASSLGRLLKNRGLNVTIQKFDPYINVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSTVLKKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKDRVFRAGKETHADVVITEIGGTVGDIESLPFLEAIRQIKSDVGRDNVMYIHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LKAAGEMKTKPTQHSVKELRSLGIQPNVIVVRTEMPISQDMKDKIALFCDIDPKAVIEAG
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCHHHEEECC
DADTLYSIPLDLQKQGLDSLVCSHLKLDCREADMEEWKELVKKVKNLSKTVTIALVGKYV
CCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECHH
ELPDAYISVVESLRHAGYAFDADIQVKWINAEEVTEDNVADLVQNADGILVPGGFGDRGV
HCCHHHHHHHHHHHHCCEEECCCEEEEEECHHHCCCCHHHHHHHCCCCEEECCCCCCCCC
EGKITTVKYAREQKIPFFGICLGMQVASIEYARNVLGLEGAHSAEIDPSTPYPIIDLLPE
CCEEEEEEEHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCC
QKDIEDLGGTLRLGLYPCKLQEGSKAYQAYENEVVYERHRHRYEFNNEFRQQMEEAGFVF
CCCHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEE
SGTSPDGRLVEIIELKDHPWFVASQFHPEFTSRPTRPQALFRDFVHASLKTSEKL
ECCCCCCCEEEEEEECCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA