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The map label for this gene is pgk

Identifier: 52787349

GI number: 52787349

Start: 3489976

End: 3491160

Strand: Reverse

Name: pgk

Synonym: BLi03664

Alternate gene names: 52787349

Gene position: 3491160-3489976 (Counterclockwise)

Preceding gene: 52787350

Following gene: 52787348

Centisome position: 82.68

GC content: 49.37

Gene sequence:

>1185_bases
ATGAACAAAAAATCAGTAAAAGACATTGATGTAAAAGGCCGCGTTGTTTTCTGCCGTGTTGACTTTAACGTTCCGATGAA
GGACGGAAAAGTGACGGATGATACGAGAATCCGCGCCGCACTTCCGACGATTCAATACTTAACAGAGCAGGGAGCGAAAG
TTCTTCTTGCAAGCCACTTGGGCCGTCCGAAAGGCCAGGTGTCAGAAGAGCTTCGCTTAACGCCGGTAGCCGCACGCCTC
GGCGAACTGATCGGCAAAGAAGTGAAAAAAGCGGATGAAGCTTACGGCGACGCCGTCAAAGCGCAAATTTCCGAAATGAA
AGACGGAGATATTCTCGTATTGGAAAATGTCCGCTTCTATCCGGGAGAAGAAAAGAATGATCCTGAGCTTTCTAAAGCGT
TTGCCGACCTGGCTGACGTTTATGTCAATGATGCATTCGGAGCGGCTCACCGCGCCCATGCTTCAACAGCAGGCATCGCT
GAACACCTTCCGGCTGTTGCAGGCTTCCTCATGGAAAAAGAGCTTGAAGTGCTGGGCAAAGCGCTGTCAAACCCTGAACG
TCCGTTTACAGCGATCATCGGCGGCGCAAAAGTAAAAGACAAAATCGGCGTGATTGAAAGTCTTCTTGAGAAAGTAGACA
ACCTGATCATCGGCGGCGGTCTTGCTTACACGTTCGTCAAAGCGCTCGGCCATGAAGTGGGAAAATCCCTGCTGGAAGAA
GATAAAATTGAATTGGCTAAATCTTTCATGGACCGCGCCAAAGAAAAAGGCGTGAACTTCTACATGCCTACAGACGTGCT
TGTTGCTGACGATTTCTCAAACGACGCCAACACGAAAATCGTGCCGGTAAGCGAAATCCCTAGCGACTGGGAAGCGCTTG
ACATCGGCGAGGAAACAAGAAAAACATATGCCGACGTCATCAAAAACAGCAAGCTTGTCGTTTGGAACGGACCGATGGGC
GTATTCGAAATCGACGCGTTCGCTAAAGGAACAAGAGCTGTCGCTGAAGCATTAGCAGAGGCAAATGATACATACTCTGT
CATCGGCGGCGGAGACTCTGCAGCTGCTGTAGAAAAGTTCGGCTTGGCTGACAAAATGAGCCACATTTCAACAGGCGGCG
GAGCTTCCCTTGAGTTTATGGAAGGAAAAGAACTTCCTGGCGTCAAAGCATTGAACGACAAGTAA

Upstream 100 bases:

>100_bases
CACACTCCCTTTCCCTTTTTCCATATCGGCGCCTTTAAGCCGCCCGGGGATTTCCGTTTTCAGGCTTGTAAAACATTAAA
AGGAGGATCTCCTAAAAGCC

Downstream 100 bases:

>100_bases
GCAAAATATACAGCATAAGGAGTGGAGCACATGAGAAAACCAATTATAGCCGGTAACTGGAAGATGAATAAAGTGCTTTC
AGAAGCTGTCAGCTTCGTTG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNKKSVKDIDVKGRVVFCRVDFNVPMKDGKVTDDTRIRAALPTIQYLTEQGAKVLLASHLGRPKGQVSEELRLTPVAARL
GELIGKEVKKADEAYGDAVKAQISEMKDGDILVLENVRFYPGEEKNDPELSKAFADLADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIESLLEKVDNLIIGGGLAYTFVKALGHEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPTDVLVADDFSNDANTKIVPVSEIPSDWEALDIGEETRKTYADVIKNSKLVVWNGPMG
VFEIDAFAKGTRAVAEALAEANDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVKALNDK

Sequences:

>Translated_394_residues
MNKKSVKDIDVKGRVVFCRVDFNVPMKDGKVTDDTRIRAALPTIQYLTEQGAKVLLASHLGRPKGQVSEELRLTPVAARL
GELIGKEVKKADEAYGDAVKAQISEMKDGDILVLENVRFYPGEEKNDPELSKAFADLADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIESLLEKVDNLIIGGGLAYTFVKALGHEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPTDVLVADDFSNDANTKIVPVSEIPSDWEALDIGEETRKTYADVIKNSKLVVWNGPMG
VFEIDAFAKGTRAVAEALAEANDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVKALNDK
>Mature_394_residues
MNKKSVKDIDVKGRVVFCRVDFNVPMKDGKVTDDTRIRAALPTIQYLTEQGAKVLLASHLGRPKGQVSEELRLTPVAARL
GELIGKEVKKADEAYGDAVKAQISEMKDGDILVLENVRFYPGEEKNDPELSKAFADLADVYVNDAFGAAHRAHASTAGIA
EHLPAVAGFLMEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIESLLEKVDNLIIGGGLAYTFVKALGHEVGKSLLEE
DKIELAKSFMDRAKEKGVNFYMPTDVLVADDFSNDANTKIVPVSEIPSDWEALDIGEETRKTYADVIKNSKLVVWNGPMG
VFEIDAFAKGTRAVAEALAEANDTYSVIGGGDSAAAVEKFGLADKMSHISTGGGASLEFMEGKELPGVKALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=46.3768115942029, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.2469733656174, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=48.2323232323232, Blast_Score=354, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=46.3592233009709, Blast_Score=359, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=48.910411622276, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=46.3768115942029, Blast_Score=345, Evalue=2e-95,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=44.5255474452555, Blast_Score=330, Evalue=1e-90,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BACLD (Q65EM9)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080752.1
- RefSeq:   YP_093178.1
- HSSP:   P18912
- ProteinModelPortal:   Q65EM9
- SMR:   Q65EM9
- STRING:   Q65EM9
- EnsemblBacteria:   EBBACT00000054356
- EnsemblBacteria:   EBBACT00000060479
- GeneID:   3029334
- GeneID:   3100633
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03664
- KEGG:   bli:BL03465
- NMPDR:   fig|279010.5.peg.2315
- eggNOG:   COG0126
- GeneTree:   EBGT00050000001506
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   BLIC279010-1:BLI03664-MONOMER
- BioCyc:   BLIC279010:BL03465-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42383; Mature: 42383

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSVKDIDVKGRVVFCRVDFNVPMKDGKVTDDTRIRAALPTIQYLTEQGAKVLLASHL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPKGQVSEELRLTPVAARLGELIGKEVKKADEAYGDAVKAQISEMKDGDILVLENVRFY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEC
PGEEKNDPELSKAFADLADVYVNDAFGAAHRAHASTAGIAEHLPAVAGFLMEKELEVLGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIESLLEKVDNLIIGGGLAYTFVKALGHEVGKSLLEE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHH
DKIELAKSFMDRAKEKGVNFYMPTDVLVADDFSNDANTKIVPVSEIPSDWEALDIGEETR
HHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCEECCCHHHH
KTYADVIKNSKLVVWNGPMGVFEIDAFAKGTRAVAEALAEANDTYSVIGGGDSAAAVEKF
HHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
GLADKMSHISTGGGASLEFMEGKELPGVKALNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNKKSVKDIDVKGRVVFCRVDFNVPMKDGKVTDDTRIRAALPTIQYLTEQGAKVLLASHL
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPKGQVSEELRLTPVAARLGELIGKEVKKADEAYGDAVKAQISEMKDGDILVLENVRFY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEC
PGEEKNDPELSKAFADLADVYVNDAFGAAHRAHASTAGIAEHLPAVAGFLMEKELEVLGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSNPERPFTAIIGGAKVKDKIGVIESLLEKVDNLIIGGGLAYTFVKALGHEVGKSLLEE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHH
DKIELAKSFMDRAKEKGVNFYMPTDVLVADDFSNDANTKIVPVSEIPSDWEALDIGEETR
HHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCEECCCHHHH
KTYADVIKNSKLVVWNGPMGVFEIDAFAKGTRAVAEALAEANDTYSVIGGGDSAAAVEKF
HHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHH
GLADKMSHISTGGGASLEFMEGKELPGVKALNDK
CCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA