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The map label for this gene is tpiA [H]

Identifier: 52787348

GI number: 52787348

Start: 3489184

End: 3489945

Strand: Reverse

Name: tpiA [H]

Synonym: BLi03663

Alternate gene names: 52787348

Gene position: 3489945-3489184 (Counterclockwise)

Preceding gene: 52787349

Following gene: 52787347

Centisome position: 82.65

GC content: 48.43

Gene sequence:

>762_bases
ATGAGAAAACCAATTATAGCCGGTAACTGGAAGATGAATAAAGTGCTTTCAGAAGCTGTCAGCTTCGTTGAAGAAGTGAA
ATCTTCCATTCCCGCAGCAGATAAAGCAGAAGCTGTTGTCTGTGCGCCTGCACTGTTCCTTGAAAAGCTCACATCAGCTG
TAAAAGGCACAGATCTGAAAGTCGGCGCGCAAAACATGCATTTTGAAGAAAGCGGAGCTTTCACGGGTGAAATCAGCCCT
GCGGCTTTGAAAGACCTTGGAGTTGAATACTGCGTCATCGGCCACTCCGAGCGCCGCGAAATGTTTGCTGAAACGGATGA
AACGGTCAACAAAAAAGCGCATGCTGCATTTAAATACGGCATCGTTCCGATCATCTGCGTAGGTGAAACGCTTGAAGAGC
GCGAAGCAAACAAAACAAACGAGCTTGTTGCAGACCAAGTGAAAAAAGCACTTGCAGGTTTAACAACTGAACAGGTAGCT
GCTTCCGTGATCGCTTATGAGCCGATCTGGGCGATTGGAACCGGCAAGTCTTCAACTGCACAAGATGCAAACGAAGTATG
CGCGCACATCCGCAAAACCGTGGCATCAGAGTTCGGACAAACTGCAGCAGACAGCGTCCGCATTCAGTACGGCGGAAGCG
TAAAACCTGCGAACATTAAAGAATATATGGCCGAATCCGATATCGACGGCGCTCTTGTTGGCGGAGCAAGCCTTGAGCCG
CAGTCTTTCGTGCAATTATTGGAGGCAGGTCAATATGAGTAA

Upstream 100 bases:

>100_bases
CGGCGGAGCTTCCCTTGAGTTTATGGAAGGAAAAGAACTTCCTGGCGTCAAAGCATTGAACGACAAGTAAGCAAAATATA
CAGCATAAGGAGTGGAGCAC

Downstream 100 bases:

>100_bases
CAAGTTAGCCGCGCTCATCATTTTAGATGGGTTTGGACTAAGGGACGAAACCGTCGGAAACGCCGTAGCCCAAGCGAAAA
AACCGAACTTTGACCGCTAT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRKPIIAGNWKMNKVLSEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLKVGAQNMHFEESGAFTGEISP
AALKDLGVEYCVIGHSERREMFAETDETVNKKAHAAFKYGIVPIICVGETLEEREANKTNELVADQVKKALAGLTTEQVA
ASVIAYEPIWAIGTGKSSTAQDANEVCAHIRKTVASEFGQTAADSVRIQYGGSVKPANIKEYMAESDIDGALVGGASLEP
QSFVQLLEAGQYE

Sequences:

>Translated_253_residues
MRKPIIAGNWKMNKVLSEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLKVGAQNMHFEESGAFTGEISP
AALKDLGVEYCVIGHSERREMFAETDETVNKKAHAAFKYGIVPIICVGETLEEREANKTNELVADQVKKALAGLTTEQVA
ASVIAYEPIWAIGTGKSSTAQDANEVCAHIRKTVASEFGQTAADSVRIQYGGSVKPANIKEYMAESDIDGALVGGASLEP
QSFVQLLEAGQYE
>Mature_253_residues
MRKPIIAGNWKMNKVLSEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLKVGAQNMHFEESGAFTGEISP
AALKDLGVEYCVIGHSERREMFAETDETVNKKAHAAFKYGIVPIICVGETLEEREANKTNELVADQVKKALAGLTTEQVA
ASVIAYEPIWAIGTGKSSTAQDANEVCAHIRKTVASEFGQTAADSVRIQYGGSVKPANIKEYMAESDIDGALVGGASLEP
QSFVQLLEAGQYE

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=42.2924901185771, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=42.2924901185771, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=40.6374501992032, Blast_Score=195, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=43.4262948207171, Blast_Score=183, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=41.7670682730924, Blast_Score=191, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28572008, Length=250, Percent_Identity=43.6, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28572006, Length=250, Percent_Identity=43.6, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=43.6, Blast_Score=195, Evalue=2e-50,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27014; Mature: 27014

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPIIAGNWKMNKVLSEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLK
CCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VGAQNMHFEESGAFTGEISPAALKDLGVEYCVIGHSERREMFAETDETVNKKAHAAFKYG
ECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
IVPIICVGETLEEREANKTNELVADQVKKALAGLTTEQVAASVIAYEPIWAIGTGKSSTA
CEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCH
QDANEVCAHIRKTVASEFGQTAADSVRIQYGGSVKPANIKEYMAESDIDGALVGGASLEP
HHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCH
QSFVQLLEAGQYE
HHHHHHHHCCCCC
>Mature Secondary Structure
MRKPIIAGNWKMNKVLSEAVSFVEEVKSSIPAADKAEAVVCAPALFLEKLTSAVKGTDLK
CCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VGAQNMHFEESGAFTGEISPAALKDLGVEYCVIGHSERREMFAETDETVNKKAHAAFKYG
ECCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
IVPIICVGETLEEREANKTNELVADQVKKALAGLTTEQVAASVIAYEPIWAIGTGKSSTA
CEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCH
QDANEVCAHIRKTVASEFGQTAADSVRIQYGGSVKPANIKEYMAESDIDGALVGGASLEP
HHHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCH
QSFVQLLEAGQYE
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA