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The map label for this gene is yugF [H]

Identifier: 52787019

GI number: 52787019

Start: 3181696

End: 3182517

Strand: Direct

Name: yugF [H]

Synonym: BLi03322

Alternate gene names: 52787019

Gene position: 3181696-3182517 (Clockwise)

Preceding gene: 52787015

Following gene: 52787022

Centisome position: 75.35

GC content: 45.62

Gene sequence:

>822_bases
ATGCTGTTAACAAAACCTTCTATCGAGACCATTCAAGGGGTCAATATATATTATGAACATTACGAGAATCCAGGCAAGAC
ATCGCTCGTTTTAATACACGGTTTTCTTTCTTCCTCGTTTTGCTACAGAAAAATCATTCCTCTTCTGAAGGATGAGTTTA
ATTTAGTCGCCGTTGACCTGCCTCCCTTCGGCCAATCAGAAAAATCCAGCACCTTTGTGTATACCTATCAAAACATGGCC
AGGGTGGTCATCGAACTGATCGAAAGGCTGAAGATTAAAGACGCCGTGCTTGTCGGTCATTCGATGGGCGGCCAGATTTC
ACTTTATGCGATAAAGGAAAGGCCTGAACTGTTCAAAAAAGTCGTCCTGCTGTGCAGCTCAGGCTATTTAAAGCGTTCGC
CCCGCTCGCTTATTTTCGGGAGCCATATTCCTTATTTTTATTTGTACATCAAGCGCTGGCTGTCAAAACAGGGCGTCCTG
AAAAACCTCATGAATGTTGTCTACGACAGCTCCCTGATCGATCAGGAAATGATCAACGGCTACCTGAAACCGTTTCTCGA
TGATCAAATTTTCAGGGCGCTCGCACGTCTTATCAGACACCGGGAAGGCGATTTATCACCGGATGATTTGAAGAAAATCG
AGACGCCTTCCCTCTTGATCTGGGGCGAAGAGGACCGCGTCGTCCCGGTACAGATCGGAAAAAGGCTTCATCAGGATCTG
CCCAATTCCATTTTCTACTCCCTGCAAGAAACCGGACACCTCGTCCCTGAAGAAAATCCGGATTATGTATCAGACAAGAT
TGCCAATTTCATTTTGACATGA

Upstream 100 bases:

>100_bases
TTTTTTTCGGGAACGATAAATAACATACTCAGGACTTAGGCGACAACATGCAGCCAGATTGTTTGAACATGAAAGAAAAA
CATTCGGAGTGATTTGGATC

Downstream 100 bases:

>100_bases
ACATAAAAAAACCGCCGGTTGGCGGTTTTTAAATTCTGTTCAGCACGAACTGCTGTCTTTGATCACAAGGAGCCGTTCAG
CCAGCCGTTTGCATTCCGAC

Product: YugF

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLLTKPSIETIQGVNIYYEHYENPGKTSLVLIHGFLSSSFCYRKIIPLLKDEFNLVAVDLPPFGQSEKSSTFVYTYQNMA
RVVIELIERLKIKDAVLVGHSMGGQISLYAIKERPELFKKVVLLCSSGYLKRSPRSLIFGSHIPYFYLYIKRWLSKQGVL
KNLMNVVYDSSLIDQEMINGYLKPFLDDQIFRALARLIRHREGDLSPDDLKKIETPSLLIWGEEDRVVPVQIGKRLHQDL
PNSIFYSLQETGHLVPEENPDYVSDKIANFILT

Sequences:

>Translated_273_residues
MLLTKPSIETIQGVNIYYEHYENPGKTSLVLIHGFLSSSFCYRKIIPLLKDEFNLVAVDLPPFGQSEKSSTFVYTYQNMA
RVVIELIERLKIKDAVLVGHSMGGQISLYAIKERPELFKKVVLLCSSGYLKRSPRSLIFGSHIPYFYLYIKRWLSKQGVL
KNLMNVVYDSSLIDQEMINGYLKPFLDDQIFRALARLIRHREGDLSPDDLKKIETPSLLIWGEEDRVVPVQIGKRLHQDL
PNSIFYSLQETGHLVPEENPDYVSDKIANFILT
>Mature_273_residues
MLLTKPSIETIQGVNIYYEHYENPGKTSLVLIHGFLSSSFCYRKIIPLLKDEFNLVAVDLPPFGQSEKSSTFVYTYQNMA
RVVIELIERLKIKDAVLVGHSMGGQISLYAIKERPELFKKVVLLCSSGYLKRSPRSLIFGSHIPYFYLYIKRWLSKQGVL
KNLMNVVYDSSLIDQEMINGYLKPFLDDQIFRALARLIRHREGDLSPDDLKKIETPSLLIWGEEDRVVPVQIGKRLHQDL
PNSIFYSLQETGHLVPEENPDYVSDKIANFILT

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=281, Percent_Identity=24.1992882562278, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI87081721, Length=263, Percent_Identity=25.8555133079848, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324392, Length=122, Percent_Identity=25.4098360655738, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31416; Mature: 31416

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTKPSIETIQGVNIYYEHYENPGKTSLVLIHGFLSSSFCYRKIIPLLKDEFNLVAVDL
CCCCCCCHHHHHCCEEEEEECCCCCCEEEEEEEEHHHCCHHHHHHHHHHCCCCCEEEEEC
PPFGQSEKSSTFVYTYQNMARVVIELIERLKIKDAVLVGHSMGGQISLYAIKERPELFKK
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHH
VVLLCSSGYLKRSPRSLIFGSHIPYFYLYIKRWLSKQGVLKNLMNVVYDSSLIDQEMING
HHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
YLKPFLDDQIFRALARLIRHREGDLSPDDLKKIETPSLLIWGEEDRVVPVQIGKRLHQDL
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHC
PNSIFYSLQETGHLVPEENPDYVSDKIANFILT
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLLTKPSIETIQGVNIYYEHYENPGKTSLVLIHGFLSSSFCYRKIIPLLKDEFNLVAVDL
CCCCCCCHHHHHCCEEEEEECCCCCCEEEEEEEEHHHCCHHHHHHHHHHCCCCCEEEEEC
PPFGQSEKSSTFVYTYQNMARVVIELIERLKIKDAVLVGHSMGGQISLYAIKERPELFKK
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCHHHHHH
VVLLCSSGYLKRSPRSLIFGSHIPYFYLYIKRWLSKQGVLKNLMNVVYDSSLIDQEMING
HHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
YLKPFLDDQIFRALARLIRHREGDLSPDDLKKIETPSLLIWGEEDRVVPVQIGKRLHQDL
HHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHC
PNSIFYSLQETGHLVPEENPDYVSDKIANFILT
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]