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The map label for this gene is yugI [H]

Identifier: 52787016

GI number: 52787016

Start: 3179225

End: 3179614

Strand: Reverse

Name: yugI [H]

Synonym: BLi03319

Alternate gene names: 52787016

Gene position: 3179614-3179225 (Counterclockwise)

Preceding gene: 52787017

Following gene: 52787014

Centisome position: 75.3

GC content: 43.08

Gene sequence:

>390_bases
ATGACAGCAAAGTTTGAAGTAGGCAGTGTTTACACTGGTAAAGTGACTGGATTACAGGCGTACGGAGCGTTTGTTGCATT
AGATGAAGAAACACAGGGACTCGTGCATATTTCTGAAGTAACACACGGTTTCGTTAAAGATATCAATGAACATTTGGCGG
TCGGTGATGAGGTGCAAGTGAAGGTTCTTTCCGTAGATGAGGAAAAAGGCAAAATCAGCCTTTCTATCCGCGCTACACAG
GCAGCTCCTGAACGAAAAGAAAGCAAGCCTAGAAGACAAAAGGCTGTTCAAGCCGAAGATACAGCTGCTCCACAAGGATT
CAACACTTTGAAAGACAAACTTGAAGAGTGGATCGAGCAGTCCAACAGAAAAGATCTTATTAAGAAGTAA

Upstream 100 bases:

>100_bases
TGCTAAAAAAAGTTTTTTTGCTTAATAAAGACTTGTATGATATAATTTTTTATTGCGTATAAAAAGAGATTAAATTCATA
AGATTTAGGAGTTGTTAGTT

Downstream 100 bases:

>100_bases
ATGAAAAAACTCCGGCAAAAGCCGGAGTTTCAGCGTGTCGACAAACCCTCGCATTCGTTGTCAGGCCTGCGCGTCGGTGC
TCACGAATTCCAACATTCGC

Product: general stress protein 13

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: GSP13 [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MTAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLAVGDEVQVKVLSVDEEKGKISLSIRATQ
AAPERKESKPRRQKAVQAEDTAAPQGFNTLKDKLEEWIEQSNRKDLIKK

Sequences:

>Translated_129_residues
MTAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLAVGDEVQVKVLSVDEEKGKISLSIRATQ
AAPERKESKPRRQKAVQAEDTAAPQGFNTLKDKLEEWIEQSNRKDLIKK
>Mature_128_residues
TAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLAVGDEVQVKVLSVDEEKGKISLSIRATQA
APERKESKPRRQKAVQAEDTAAPQGFNTLKDKLEEWIEQSNRKDLIKK

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI145693187, Length=77, Percent_Identity=50.6493506493506, Blast_Score=73, Evalue=5e-15,
Organism=Escherichia coli, GI87082262, Length=73, Percent_Identity=39.7260273972603, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR000110
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 14280; Mature: 14149

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLAVGDEVQV
CCCCCCCCCEEECCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEE
KVLSVDEEKGKISLSIRATQAAPERKESKPRRQKAVQAEDTAAPQGFNTLKDKLEEWIEQ
EEEEECCCCCEEEEEEEECCCCCCCHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SNRKDLIKK
CCHHHHCCC
>Mature Secondary Structure 
TAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLAVGDEVQV
CCCCCCCCEEECCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEE
KVLSVDEEKGKISLSIRATQAAPERKESKPRRQKAVQAEDTAAPQGFNTLKDKLEEWIEQ
EEEEECCCCCEEEEEEEECCCCCCCHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SNRKDLIKK
CCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 9298659 [H]