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The map label for this gene is yugJ [H]

Identifier: 52787014

GI number: 52787014

Start: 3177450

End: 3178613

Strand: Reverse

Name: yugJ [H]

Synonym: BLi03317

Alternate gene names: 52787014

Gene position: 3178613-3177450 (Counterclockwise)

Preceding gene: 52787016

Following gene: 52787013

Centisome position: 75.28

GC content: 48.28

Gene sequence:

>1164_bases
ATGGATAACTTTACATATTGGAATCCCACAAAGCTGATATTCGGCCGGGGAGAAGTTGAAAAGCTGGCAGAAGAGGTGAA
ACAATACGGCCGCAATGTCCTGCTCGTATACGGCGGAGGCAGCATTAAGCGAAACGGTTTATACGATCAAGTCATTTCAA
TCCTTGAAAAGGCGGGCGCGACCGTCCATGAACTGCCCGGCGTCGAACCGAATCCGCGTGTTGCCACTGTGAATAAAGGA
GTTGCGATCTGCAAAGAGAACGATATTGACTTTCTTTTGGCAGTCGGCGGCGGAAGCGTCATTGATTGTACGAAAGCAAT
TGCTGCCGGAGCGAAATACGACGGCGATGCGTGGGATATTGTGACGAAAAAACATATTCCGGCTGATGCGCTGCCGTTTG
GAACAGTTTTAACGTTAGCAGCAACAGGCTCTGAAATGAACTCGGGATCTGTGATCACAAATTGGGAAACCAATGAAAAA
TACGGCTGGGGAAGCCCGCTCGTATTCCCTAAATTTTCAATTCTTGATCCGGTCAACACGTTTACCGTCCCGAAAGACCA
CACGATTTACGGCATTGTCGACATGATGTCCCACGTGTTTGAGCAATATTTTCACCATACCGAAAATACCCCTTATCAGG
ACCGGATGTGCGAATCCCTGCTTAAAACGGTAATTGAAACAGCTCCTAAGCTCATTGAAGACCTAGAAAACTATGAGCTG
CGTGAAACGATTCTGTATACAGGCACCATTGCGCTGAACGGCATGCTATCAATGGGCGCACGCGGAGACTGGGCAACGCA
CAATATCGAGCACGCTGTTTCAGCCGTATACGATATTCCGCATGCGGGAGGGCTTGCGATTCTGTTCCCGAATTGGATGA
AGCACACGCTTTCCGAGAACGTCGGCCGCTTTAAACAGCTTGCCGTCCGTGTTTTTGACGTAGATGAAACAGGAAAAACC
GATCGCGAAGTGGCGCTTGTTGGAATCGAGAAACTGTCTGAATTCTGGACCAGCCTTGGCGCGCCAAATCGTCTTGCCGA
TTATGACATTACAGATGAGAAGCTTGATCTCATTGCCGACAAAGCGATGGCAAACGGCGAATTCGGCCGCTTTAAAACGC
TGAATAAAGACGATGTTCTGTCTATTTTGAAGGCTTCTTTATAA

Upstream 100 bases:

>100_bases
AGATTGCGACCGATTTGGAAACATTATACGCCTGAGGAGCAGATCGCTTTACAGAGCGTTCGGCGATTTCCCATAATAGT
AAATAAAGGAGGAGATGTAG

Downstream 100 bases:

>100_bases
GATTTCTTGACGGCTCAAGGAGAACCGCCATTCCTTGAGCCGTTGTTTATTGTTATGTTTTTGACAAAAATGCAAGTGGA
GGCTGAAAAAACATGCATTT

Product: YugJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MDNFTYWNPTKLIFGRGEVEKLAEEVKQYGRNVLLVYGGGSIKRNGLYDQVISILEKAGATVHELPGVEPNPRVATVNKG
VAICKENDIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHIPADALPFGTVLTLAATGSEMNSGSVITNWETNEK
YGWGSPLVFPKFSILDPVNTFTVPKDHTIYGIVDMMSHVFEQYFHHTENTPYQDRMCESLLKTVIETAPKLIEDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMKHTLSENVGRFKQLAVRVFDVDETGKT
DREVALVGIEKLSEFWTSLGAPNRLADYDITDEKLDLIADKAMANGEFGRFKTLNKDDVLSILKASL

Sequences:

>Translated_387_residues
MDNFTYWNPTKLIFGRGEVEKLAEEVKQYGRNVLLVYGGGSIKRNGLYDQVISILEKAGATVHELPGVEPNPRVATVNKG
VAICKENDIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHIPADALPFGTVLTLAATGSEMNSGSVITNWETNEK
YGWGSPLVFPKFSILDPVNTFTVPKDHTIYGIVDMMSHVFEQYFHHTENTPYQDRMCESLLKTVIETAPKLIEDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMKHTLSENVGRFKQLAVRVFDVDETGKT
DREVALVGIEKLSEFWTSLGAPNRLADYDITDEKLDLIADKAMANGEFGRFKTLNKDDVLSILKASL
>Mature_387_residues
MDNFTYWNPTKLIFGRGEVEKLAEEVKQYGRNVLLVYGGGSIKRNGLYDQVISILEKAGATVHELPGVEPNPRVATVNKG
VAICKENDIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHIPADALPFGTVLTLAATGSEMNSGSVITNWETNEK
YGWGSPLVFPKFSILDPVNTFTVPKDHTIYGIVDMMSHVFEQYFHHTENTPYQDRMCESLLKTVIETAPKLIEDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMKHTLSENVGRFKQLAVRVFDVDETGKT
DREVALVGIEKLSEFWTSLGAPNRLADYDITDEKLDLIADKAMANGEFGRFKTLNKDDVLSILKASL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=370, Percent_Identity=38.9189189189189, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI48994951, Length=388, Percent_Identity=25.5154639175258, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87082107, Length=350, Percent_Identity=25.1428571428571, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789163, Length=367, Percent_Identity=24.5231607629428, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787493, Length=333, Percent_Identity=24.024024024024, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=200, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321181, Length=384, Percent_Identity=24.4791666666667, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 42723; Mature: 42723

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFTYWNPTKLIFGRGEVEKLAEEVKQYGRNVLLVYGGGSIKRNGLYDQVISILEKAGA
CCCCCEECCCEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
TVHELPGVEPNPRVATVNKGVAICKENDIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDI
CHHHCCCCCCCCCEEEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
VTKKHIPADALPFGTVLTLAATGSEMNSGSVITNWETNEKYGWGSPLVFPKFSILDPVNT
EECCCCCCCCCCCHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHCCCCCC
FTVPKDHTIYGIVDMMSHVFEQYFHHTENTPYQDRMCESLLKTVIETAPKLIEDLENYEL
CCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMKHTLSEN
HHHHEEEEHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
VGRFKQLAVRVFDVDETGKTDREVALVGIEKLSEFWTSLGAPNRLADYDITDEKLDLIAD
HHHHHHHHEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KAMANGEFGRFKTLNKDDVLSILKASL
HHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDNFTYWNPTKLIFGRGEVEKLAEEVKQYGRNVLLVYGGGSIKRNGLYDQVISILEKAGA
CCCCCEECCCEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
TVHELPGVEPNPRVATVNKGVAICKENDIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDI
CHHHCCCCCCCCCEEEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
VTKKHIPADALPFGTVLTLAATGSEMNSGSVITNWETNEKYGWGSPLVFPKFSILDPVNT
EECCCCCCCCCCCHHHEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHCCCCCC
FTVPKDHTIYGIVDMMSHVFEQYFHHTENTPYQDRMCESLLKTVIETAPKLIEDLENYEL
CCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMKHTLSEN
HHHHEEEEHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
VGRFKQLAVRVFDVDETGKTDREVALVGIEKLSEFWTSLGAPNRLADYDITDEKLDLIAD
HHHHHHHHEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KAMANGEFGRFKTLNKDDVLSILKASL
HHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]