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The map label for this gene is opuD [H]

Identifier: 52786882

GI number: 52786882

Start: 3039591

End: 3041129

Strand: Direct

Name: opuD [H]

Synonym: BLi03157

Alternate gene names: 52786882

Gene position: 3039591-3041129 (Clockwise)

Preceding gene: 52786881

Following gene: 52786892

Centisome position: 71.98

GC content: 47.37

Gene sequence:

>1539_bases
ATGATTTTGAAAAATATTTCAAGCGTTTTTTGGATTGTGATTGTTATCACAGCAGCAGCTGTATTATGGGGAATCGTTGC
TCCGGATAACCTTCAGGCGTTAACGACTTCGATTCAAGGTTATGTTACAAACACACTCGGATGGTATTACTTATTGGTGG
TTTCTCTGTTTGTCGCATTCAGCATCTTTTTGATCTTCAGTCCGATTGGAAAGATCAGATTAGGGAAACCTGAAGAAAAA
CCTGAATTCTCTCGATTATCTTGGTTTGCCATGCTTTTTAGCGCAGGTATGGGCATTGGTCTCGTATTTTATGGCGCGGC
TGAACCGATCAGCCATTATGCCATTAGCTCTCCATCAGGTGAAACAGAAACAGCCCAGGCCTTCAGAGATTCTCTGAGGT
ATACATTTTTCCACTGGGGATTGCATGCTTGGGCGATCTATGCGATCGTTGCCCTTTGCATCGCATACTTTCAATTCAGA
AAAGACGCGCCTGGCTTGATCAGCGCGACGCTGTTTCCGGTCTTCGGCGACAGAGTCAACGGACCGCTTGGAAAAGGGAT
TGACTGCATCGCTGTATTTGCAACCGTCGTCGGTGTAGCGACTAGTCTCGGAATCGGCGCCACGCAGATCAACGGCGGAC
TCCATTATCTATTCGGAGTGCCGAACAGCTTCCAGGTGCAGCTGGCGATCATTGCAGTCATCACCGTTCTGTTTTTAATT
TCCGCTTGGAGCGGAATCGGAAAAGGCATCAAGTATTTGAGCAATACGAACATGGTGCTGGCCGGCGTCCTGATGATCTT
TATGCTGTTTGTCGGACCGACGGTGTTAATTATGAACTCGTTTACGGACACGATCGGAACGTATATCTCCAATGTGGTGC
AAATGAGCTTCCGCCTTGCGCCTAATGATCCGGACAGCCGTGAATGGATCAATTCATGGACGATTTTCTACTGGGCTTGG
TGGATCTCCTGGTCGCCGTTTGTCGGTATTTTCATCGCCCGCGTATCAAGGGGGCGCACAATCAGGGAATTTTTGATTGC
CGTCTTGTTTGCGCCTACTGTGCTGGCGTTTCTATGGTTTTCAATTTTCGGGGTTTCAGCGATGGATCTTCAGCAAAAAG
GGATTTTCAACGTTGCAAAACTGTCTACAGACACGATGCTGTTTGGAATGTTTGACCACTACCCGCTGGCTATGATTACA
TCTATCGTTGCCTTGTTGCTCATTGCCGTATTCTTTATCACTTCTGCAGACTCGGCAACATTTGTTCTCGGAATGCAAAC
GACTTACGGTTCACTGAATCCTGAGAATTCGGTCAAGATATCATGGGGCATCATCCAGTCAGCAATGGCTGGGGCGCTCC
TGTATTCAGGCGGACTATCGGCGCTGCAAAACACCGCCATCCTGGCCGCACTGCCGTTCTCAGTCGTCGTGATTCTCATG
ATTGTATCGTTATATAAATCTCTGTCACAAGAACGAAAAGAGATTAAACAAGCCGAGAAATTCCAGAAGCCAAGAAGCCC
GAGGGTGAAAAAAGCGTAA

Upstream 100 bases:

>100_bases
AAGCTAGGTCAAACTTCCTGAGAGAGAATGATTTTTATATGTGCAACTCATACTAAACATAATGATGGGAGTAAGGACGA
AAAATGATTAGAGGGTGAGG

Downstream 100 bases:

>100_bases
ATGAAAACAGCTCTTCTCTCTGAAGAGCTGTTTTTTTATGTCCTGTATAAGATTAACGCTGAAAAACAGTAAAGCTGTTC
GCCTTGAGGATCGCATGCGG

Product: OpuD

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MILKNISSVFWIVIVITAAAVLWGIVAPDNLQALTTSIQGYVTNTLGWYYLLVVSLFVAFSIFLIFSPIGKIRLGKPEEK
PEFSRLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFQFR
KDAPGLISATLFPVFGDRVNGPLGKGIDCIAVFATVVGVATSLGIGATQINGGLHYLFGVPNSFQVQLAIIAVITVLFLI
SAWSGIGKGIKYLSNTNMVLAGVLMIFMLFVGPTVLIMNSFTDTIGTYISNVVQMSFRLAPNDPDSREWINSWTIFYWAW
WISWSPFVGIFIARVSRGRTIREFLIAVLFAPTVLAFLWFSIFGVSAMDLQQKGIFNVAKLSTDTMLFGMFDHYPLAMIT
SIVALLLIAVFFITSADSATFVLGMQTTYGSLNPENSVKISWGIIQSAMAGALLYSGGLSALQNTAILAALPFSVVVILM
IVSLYKSLSQERKEIKQAEKFQKPRSPRVKKA

Sequences:

>Translated_512_residues
MILKNISSVFWIVIVITAAAVLWGIVAPDNLQALTTSIQGYVTNTLGWYYLLVVSLFVAFSIFLIFSPIGKIRLGKPEEK
PEFSRLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFQFR
KDAPGLISATLFPVFGDRVNGPLGKGIDCIAVFATVVGVATSLGIGATQINGGLHYLFGVPNSFQVQLAIIAVITVLFLI
SAWSGIGKGIKYLSNTNMVLAGVLMIFMLFVGPTVLIMNSFTDTIGTYISNVVQMSFRLAPNDPDSREWINSWTIFYWAW
WISWSPFVGIFIARVSRGRTIREFLIAVLFAPTVLAFLWFSIFGVSAMDLQQKGIFNVAKLSTDTMLFGMFDHYPLAMIT
SIVALLLIAVFFITSADSATFVLGMQTTYGSLNPENSVKISWGIIQSAMAGALLYSGGLSALQNTAILAALPFSVVVILM
IVSLYKSLSQERKEIKQAEKFQKPRSPRVKKA
>Mature_512_residues
MILKNISSVFWIVIVITAAAVLWGIVAPDNLQALTTSIQGYVTNTLGWYYLLVVSLFVAFSIFLIFSPIGKIRLGKPEEK
PEFSRLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSGETETAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFQFR
KDAPGLISATLFPVFGDRVNGPLGKGIDCIAVFATVVGVATSLGIGATQINGGLHYLFGVPNSFQVQLAIIAVITVLFLI
SAWSGIGKGIKYLSNTNMVLAGVLMIFMLFVGPTVLIMNSFTDTIGTYISNVVQMSFRLAPNDPDSREWINSWTIFYWAW
WISWSPFVGIFIARVSRGRTIREFLIAVLFAPTVLAFLWFSIFGVSAMDLQQKGIFNVAKLSTDTMLFGMFDHYPLAMIT
SIVALLLIAVFFITSADSATFVLGMQTTYGSLNPENSVKISWGIIQSAMAGALLYSGGLSALQNTAILAALPFSVVVILM
IVSLYKSLSQERKEIKQAEKFQKPRSPRVKKA

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=474, Percent_Identity=39.4514767932489, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788102, Length=466, Percent_Identity=31.5450643776824, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1786224, Length=479, Percent_Identity=30.062630480167, Blast_Score=202, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56243; Mature: 56243

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKNISSVFWIVIVITAAAVLWGIVAPDNLQALTTSIQGYVTNTLGWYYLLVVSLFVAF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFLIFSPIGKIRLGKPEEKPEFSRLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSG
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHEECCCCC
ETETAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFQFRKDAPGLISATLFPVFGDRVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GPLGKGIDCIAVFATVVGVATSLGIGATQINGGLHYLFGVPNSFQVQLAIIAVITVLFLI
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
SAWSGIGKGIKYLSNTNMVLAGVLMIFMLFVGPTVLIMNSFTDTIGTYISNVVQMSFRLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PNDPDSREWINSWTIFYWAWWISWSPFVGIFIARVSRGRTIREFLIAVLFAPTVLAFLWF
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SIFGVSAMDLQQKGIFNVAKLSTDTMLFGMFDHYPLAMITSIVALLLIAVFFITSADSAT
HHHCCCHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
FVLGMQTTYGSLNPENSVKISWGIIQSAMAGALLYSGGLSALQNTAILAALPFSVVVILM
EEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IVSLYKSLSQERKEIKQAEKFQKPRSPRVKKA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MILKNISSVFWIVIVITAAAVLWGIVAPDNLQALTTSIQGYVTNTLGWYYLLVVSLFVAF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFLIFSPIGKIRLGKPEEKPEFSRLSWFAMLFSAGMGIGLVFYGAAEPISHYAISSPSG
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHEECCCCC
ETETAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFQFRKDAPGLISATLFPVFGDRVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GPLGKGIDCIAVFATVVGVATSLGIGATQINGGLHYLFGVPNSFQVQLAIIAVITVLFLI
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
SAWSGIGKGIKYLSNTNMVLAGVLMIFMLFVGPTVLIMNSFTDTIGTYISNVVQMSFRLA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PNDPDSREWINSWTIFYWAWWISWSPFVGIFIARVSRGRTIREFLIAVLFAPTVLAFLWF
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SIFGVSAMDLQQKGIFNVAKLSTDTMLFGMFDHYPLAMITSIVALLLIAVFFITSADSAT
HHHCCCHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
FVLGMQTTYGSLNPENSVKISWGIIQSAMAGALLYSGGLSALQNTAILAALPFSVVVILM
EEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
IVSLYKSLSQERKEIKQAEKFQKPRSPRVKKA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]