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The map label for this gene is amyX [H]

Identifier: 52786869

GI number: 52786869

Start: 3025290

End: 3027422

Strand: Reverse

Name: amyX [H]

Synonym: BLi03144

Alternate gene names: 52786869

Gene position: 3027422-3025290 (Counterclockwise)

Preceding gene: 52786870

Following gene: 52786868

Centisome position: 71.69

GC content: 47.96

Gene sequence:

>2133_bases
ATGCCGGGTATCAGCCGCCCTTTTGAAGCTTATCTCGATGAAATGAGAACGATCACTGTTTTAGTTCCGAAGAGCCGTGC
GTCAAGCTGCAGTCCTCCTTTTCTGCTTGAAGATGATCAAGGAGAGAGGATAGAGCTTTCGGTAAAAGCGCAGGTGGAGC
TTGAGGAACAATTCAAGTATGTTCTTGAAAGCAGCTGCACCGTTCCATTCGGAAGAGTTCACAAAGTATGCTGTGAAGAA
AGCGTCTGGACAGACCTGCAAATCGGCTCTGTTACGAGAAGCGCGGCGTTTGACAAGGCTTTTTTTTATGACGGCCGCCT
CGGCGCTTTTTATTCAAAAGGAAGTACTTTATTTAAGGTATGGGCGCCGACCGCGAGTGCGGCAGCGATCAAGCTGGAGG
ACCCTGATTCGCTTCAAACAAATACTTTTCAAATGATGCGCAGAAAAAAGGGCGTTTTTGAGGTAACGGTCGAAGGCGAT
CTAAACGGCTGGTCCTATTTATATGAGCTTTATGTAAATGGGAAGCCGTTATTGACAGTCGATCCTTATGCAAAAGCCGT
TACGGCAAACGGTGAAAAAGGCGTTGTATTAGATCCAGAGGAAGTCAAGGTGGAAAAACACCGCGCTCCAAGACTTCACA
GTCCGTGCGATGCGGTTATATATGAGGTTCACATCCGCGATTTCTCGATTCATGAAGACAGCGGTATGCGCCATAAAGGC
AAATATGTTGCTTTTACTGAGGACGGAACTGAAACATCCGGCGGCTTCTCAACGGGAATCGCCTATTTGAAAGAGCTCGG
CGTCACCCACATCGAGGTTCTGCCCTTTCACGATTTTGCCGGAGTGGATGAGCTGTCTCCTGATCAATCTTATAATTGGG
GCTATAACCCCCTCCATTTTAATGCCCCGGAAGGAAGTTATTCGCTCGATCCGCAGAACCCCAAATGCAGAATCACCGAG
CTGAAAACGATGATTCAGTCGCTGCACAAACACGGCTTCTCCGTGATTATGGATGCCGTATATAATCATGTGTACAAGAG
GGAAACGTCCCCTTTTGAAAAAACGGTTCCCGGGTATTTTTTCAGACATAATGAATACGGTTTTCCATCCGACGGAACAG
GCGTCGGAAATGATATTGCTTCTGAGCGGCTGATGGTGCGAAAATATATTCTCGATTCTGTCCGCTATTGGCTGGAAGAA
TATGATGTCGACGGCATTCGTTTTGATTTAATGGGCATTCTTGATATTGAAACCGTCCGCCAAATCAGTACGCTTGCCGA
GAATGTAAAGCCGGGCGTGCCTCTGTTCGGAGAGGGCTGGGATTTAAATACCCCGCTTGACAGCGGGCAAAAAGCAACAT
TGCAAAATGCCGGAAAAGTTCCGGCGGTCGGCTTTTTTAATGATCGGTTCAGGAACGCAGTCAAAGGGAGTACATTCGAG
CTTAGCGACCGCGGATATGCTTTAGGGGACACCGGAAAAAAAGCGGCGCTTCAGCACGGTATTGCCGGTTCACCCGGATT
CTTGCAGCCGGCACAGTCAATCAATTATGTGGAATGCCATGACAATCATACATTTTGGGATAAAATGGCCTTATGTTTTG
AAGAAGATGCGGACACTAAACGGCTTAGGCAAAGGCTCGCGGTGTCGATCGTCCTGCTTTCGCAAGGCGTTCCATTTCTT
CACGCCGGGCAGGAATTTTGCCGAACAAAGAATGGGGACAGCAACAGCTACAGGTCCGGAGATGACATCAATAAGCTTGA
TTGGGAAAAGCGTGCGGAGCTTTGCGAGGATGTCGAATATGTGCGCCAGCTGATCCGGCTGCGAAGAAGCCATCCCGCTT
TTCGTCTGCAAAAAGAGGAAGAAGTCAAGGAGCATCTTTCTTTTATGGACGGTACAGGAGAGGTAACGGCCTACAAGCTG
AAGAACATTGCAGCAATTGATCCCTGGAACGAAATCATAGTGGTGCATTGTCCGTTTGCAAAAAAGGAGACGCTTAAGCT
GCCCGATCAGAAACAGTACTTGCTGCACTGCGATCCTTTTACGTTCTTCAATGGGAAGGTTCAAGCGGAAAAAAGGCTTC
GGCTGAACGGCATCGGGACCTATGTTTTATACGAACCAAAAGGGATTTTTTAG

Upstream 100 bases:

>100_bases
CGGATGGAGAGGTGATCGGCTGGGCGCCCGTTTTCATTAAAACAGGGACTGAATCCCTTCGATTAAGAGCATAAATCTTT
CGAATGAAGGAGAGACTGAA

Downstream 100 bases:

>100_bases
TTTTGCTTGACGAAGTTTGGCGTCTAGCGCTAAAATTTAAAAGACGGCTATTGTTTAGCACAAATGACTCTCAATAGCCG
TCTTTTTATTTGAATATAAA

Product: AmyX

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MPGISRPFEAYLDEMRTITVLVPKSRASSCSPPFLLEDDQGERIELSVKAQVELEEQFKYVLESSCTVPFGRVHKVCCEE
SVWTDLQIGSVTRSAAFDKAFFYDGRLGAFYSKGSTLFKVWAPTASAAAIKLEDPDSLQTNTFQMMRRKKGVFEVTVEGD
LNGWSYLYELYVNGKPLLTVDPYAKAVTANGEKGVVLDPEEVKVEKHRAPRLHSPCDAVIYEVHIRDFSIHEDSGMRHKG
KYVAFTEDGTETSGGFSTGIAYLKELGVTHIEVLPFHDFAGVDELSPDQSYNWGYNPLHFNAPEGSYSLDPQNPKCRITE
LKTMIQSLHKHGFSVIMDAVYNHVYKRETSPFEKTVPGYFFRHNEYGFPSDGTGVGNDIASERLMVRKYILDSVRYWLEE
YDVDGIRFDLMGILDIETVRQISTLAENVKPGVPLFGEGWDLNTPLDSGQKATLQNAGKVPAVGFFNDRFRNAVKGSTFE
LSDRGYALGDTGKKAALQHGIAGSPGFLQPAQSINYVECHDNHTFWDKMALCFEEDADTKRLRQRLAVSIVLLSQGVPFL
HAGQEFCRTKNGDSNSYRSGDDINKLDWEKRAELCEDVEYVRQLIRLRRSHPAFRLQKEEEVKEHLSFMDGTGEVTAYKL
KNIAAIDPWNEIIVVHCPFAKKETLKLPDQKQYLLHCDPFTFFNGKVQAEKRLRLNGIGTYVLYEPKGIF

Sequences:

>Translated_710_residues
MPGISRPFEAYLDEMRTITVLVPKSRASSCSPPFLLEDDQGERIELSVKAQVELEEQFKYVLESSCTVPFGRVHKVCCEE
SVWTDLQIGSVTRSAAFDKAFFYDGRLGAFYSKGSTLFKVWAPTASAAAIKLEDPDSLQTNTFQMMRRKKGVFEVTVEGD
LNGWSYLYELYVNGKPLLTVDPYAKAVTANGEKGVVLDPEEVKVEKHRAPRLHSPCDAVIYEVHIRDFSIHEDSGMRHKG
KYVAFTEDGTETSGGFSTGIAYLKELGVTHIEVLPFHDFAGVDELSPDQSYNWGYNPLHFNAPEGSYSLDPQNPKCRITE
LKTMIQSLHKHGFSVIMDAVYNHVYKRETSPFEKTVPGYFFRHNEYGFPSDGTGVGNDIASERLMVRKYILDSVRYWLEE
YDVDGIRFDLMGILDIETVRQISTLAENVKPGVPLFGEGWDLNTPLDSGQKATLQNAGKVPAVGFFNDRFRNAVKGSTFE
LSDRGYALGDTGKKAALQHGIAGSPGFLQPAQSINYVECHDNHTFWDKMALCFEEDADTKRLRQRLAVSIVLLSQGVPFL
HAGQEFCRTKNGDSNSYRSGDDINKLDWEKRAELCEDVEYVRQLIRLRRSHPAFRLQKEEEVKEHLSFMDGTGEVTAYKL
KNIAAIDPWNEIIVVHCPFAKKETLKLPDQKQYLLHCDPFTFFNGKVQAEKRLRLNGIGTYVLYEPKGIF
>Mature_709_residues
PGISRPFEAYLDEMRTITVLVPKSRASSCSPPFLLEDDQGERIELSVKAQVELEEQFKYVLESSCTVPFGRVHKVCCEES
VWTDLQIGSVTRSAAFDKAFFYDGRLGAFYSKGSTLFKVWAPTASAAAIKLEDPDSLQTNTFQMMRRKKGVFEVTVEGDL
NGWSYLYELYVNGKPLLTVDPYAKAVTANGEKGVVLDPEEVKVEKHRAPRLHSPCDAVIYEVHIRDFSIHEDSGMRHKGK
YVAFTEDGTETSGGFSTGIAYLKELGVTHIEVLPFHDFAGVDELSPDQSYNWGYNPLHFNAPEGSYSLDPQNPKCRITEL
KTMIQSLHKHGFSVIMDAVYNHVYKRETSPFEKTVPGYFFRHNEYGFPSDGTGVGNDIASERLMVRKYILDSVRYWLEEY
DVDGIRFDLMGILDIETVRQISTLAENVKPGVPLFGEGWDLNTPLDSGQKATLQNAGKVPAVGFFNDRFRNAVKGSTFEL
SDRGYALGDTGKKAALQHGIAGSPGFLQPAQSINYVECHDNHTFWDKMALCFEEDADTKRLRQRLAVSIVLLSQGVPFLH
AGQEFCRTKNGDSNSYRSGDDINKLDWEKRAELCEDVEYVRQLIRLRRSHPAFRLQKEEEVKEHLSFMDGTGEVTAYKLK
NIAAIDPWNEIIVVHCPFAKKETLKLPDQKQYLLHCDPFTFFNGKVQAEKRLRLNGIGTYVLYEPKGIF

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=442, Percent_Identity=27.6018099547511, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1789839, Length=306, Percent_Identity=25.1633986928105, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 80260; Mature: 80128

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGISRPFEAYLDEMRTITVLVPKSRASSCSPPFLLEDDQGERIELSVKAQVELEEQFKY
CCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHH
VLESSCTVPFGRVHKVCCEESVWTDLQIGSVTRSAAFDKAFFYDGRLGAFYSKGSTLFKV
HHHCCCCCCHHHHHHHHHCHHHCCCEEECCCHHHHHHCHHEEECCCCCEEECCCCEEEEE
WAPTASAAAIKLEDPDSLQTNTFQMMRRKKGVFEVTVEGDLNGWSYLYELYVNGKPLLTV
ECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCEEEEE
DPYAKAVTANGEKGVVLDPEEVKVEKHRAPRLHSPCDAVIYEVHIRDFSIHEDSGMRHKG
CCCHHEEECCCCCCEEECHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCC
KYVAFTEDGTETSGGFSTGIAYLKELGVTHIEVLPFHDFAGVDELSPDQSYNWGYNPLHF
CEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
NAPEGSYSLDPQNPKCRITELKTMIQSLHKHGFSVIMDAVYNHVYKRETSPFEKTVPGYF
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCCEE
FRHNEYGFPSDGTGVGNDIASERLMVRKYILDSVRYWLEEYDVDGIRFDLMGILDIETVR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
QISTLAENVKPGVPLFGEGWDLNTPLDSGQKATLQNAGKVPAVGFFNDRFRNAVKGSTFE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCCHHHHHHHHCCCEEE
LSDRGYALGDTGKKAALQHGIAGSPGFLQPAQSINYVECHDNHTFWDKMALCFEEDADTK
ECCCCEEECCCCHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHHHHCCCCCHH
RLRQRLAVSIVLLSQGVPFLHAGQEFCRTKNGDSNSYRSGDDINKLDWEKRAELCEDVEY
HHHHHHHHEEEEECCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VRQLIRLRRSHPAFRLQKEEEVKEHLSFMDGTGEVTAYKLKNIAAIDPWNEIIVVHCPFA
HHHHHHHHHCCCCEECCCHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCEEEEECCCC
KKETLKLPDQKQYLLHCDPFTFFNGKVQAEKRLRLNGIGTYVLYEPKGIF
CCCCCCCCCCCCEEEEECCEEEECCEEECCCEEEEECCEEEEEECCCCCC
>Mature Secondary Structure 
PGISRPFEAYLDEMRTITVLVPKSRASSCSPPFLLEDDQGERIELSVKAQVELEEQFKY
CCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHH
VLESSCTVPFGRVHKVCCEESVWTDLQIGSVTRSAAFDKAFFYDGRLGAFYSKGSTLFKV
HHHCCCCCCHHHHHHHHHCHHHCCCEEECCCHHHHHHCHHEEECCCCCEEECCCCEEEEE
WAPTASAAAIKLEDPDSLQTNTFQMMRRKKGVFEVTVEGDLNGWSYLYELYVNGKPLLTV
ECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCEEEEE
DPYAKAVTANGEKGVVLDPEEVKVEKHRAPRLHSPCDAVIYEVHIRDFSIHEDSGMRHKG
CCCHHEEECCCCCCEEECHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCC
KYVAFTEDGTETSGGFSTGIAYLKELGVTHIEVLPFHDFAGVDELSPDQSYNWGYNPLHF
CEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
NAPEGSYSLDPQNPKCRITELKTMIQSLHKHGFSVIMDAVYNHVYKRETSPFEKTVPGYF
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCCEE
FRHNEYGFPSDGTGVGNDIASERLMVRKYILDSVRYWLEEYDVDGIRFDLMGILDIETVR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
QISTLAENVKPGVPLFGEGWDLNTPLDSGQKATLQNAGKVPAVGFFNDRFRNAVKGSTFE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCCHHHHHHHHCCCEEE
LSDRGYALGDTGKKAALQHGIAGSPGFLQPAQSINYVECHDNHTFWDKMALCFEEDADTK
ECCCCEEECCCCHHHHHHCCCCCCCCCCCHHCCCCEEEECCCCCHHHHHHHHHCCCCCHH
RLRQRLAVSIVLLSQGVPFLHAGQEFCRTKNGDSNSYRSGDDINKLDWEKRAELCEDVEY
HHHHHHHHEEEEECCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VRQLIRLRRSHPAFRLQKEEEVKEHLSFMDGTGEVTAYKLKNIAAIDPWNEIIVVHCPFA
HHHHHHHHHCCCCEECCCHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCEEEEECCCC
KKETLKLPDQKQYLLHCDPFTFFNGKVQAEKRLRLNGIGTYVLYEPKGIF
CCCCCCCCCCCCEEEEECCEEEECCEEECCCEEEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]