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The map label for this gene is trmB

Identifier: 52786866

GI number: 52786866

Start: 3023203

End: 3023844

Strand: Reverse

Name: trmB

Synonym: BLi03141

Alternate gene names: 52786866

Gene position: 3023844-3023203 (Counterclockwise)

Preceding gene: 52786868

Following gene: 52786865

Centisome position: 71.61

GC content: 46.42

Gene sequence:

>642_bases
ATGCGAATGCGTCACAAGCCATGGGCGGATGACTACCTGGCTGAAAACTCTCATATTGTGATCAGCGAGCCGTCTCAATA
CAAAGGGAAATGGCATTCCGTTTTTGGAAATGACAACCCGATCCACATCGAAGTAGGAACCGGAAAAGGGCAATTTATTT
CAGGAATGGCCCTGCAAAACCCGGATGTAAACTATATCGGAATCGAACTATTTAAAAGCGTCATCGTAACCGCTGTTGAT
AAAGTAAAGCAGACAGAGGCGCCGAATGTAAAGCTCTTGAACATCAATGCCAATATGCTTTCAGACGTTTTTGCAGACGG
GGAAGTCGACCGGGTCTATTTGAATTTTTCCGACCCGTGGCCGAAAAAGCGCCATGAAAAGCGCCGTCTTACCAATCATG
CGTTCTTGAAAAAGTATGAGCAGGTGCTCGGAGGCAAGGGAGCGATTCATTTTAAAACCGATAACCGCGGGTTGTTTGAG
TACTCCCTCACCAGTTTTTCCGAATACGGACTCGTTTTGACATTCGTCAGCCTTGACCTGCATCAAAGCGATTTTGAAGG
AAATGTGATGACCGAATACGAAGAAAAGTTCGCCGCTAAAGGCCAGCCGATCTACAGAGTCGAAGCTGAATGGCGAACAT
AA

Upstream 100 bases:

>100_bases
AGGCCGATCTTCGGTCTTTTTTGCTTTGGAAGAGGCCTTGCGGGATTCCTGCGTTTCATGGTATGTTGTAAGACGATATA
ACAGATAGAGGTGTAAGTAC

Downstream 100 bases:

>100_bases
AAAAACAGACTGCTTCCTGCAGTCTGTTTTTTTGCTTTCAAATGAAGCTGATTCAGGTTGCATTTTTTGCTACGATAGGA
AGAGGAGGGATGATCATGGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRMRHKPWADDYLAENSHIVISEPSQYKGKWHSVFGNDNPIHIEVGTGKGQFISGMALQNPDVNYIGIELFKSVIVTAVD
KVKQTEAPNVKLLNINANMLSDVFADGEVDRVYLNFSDPWPKKRHEKRRLTNHAFLKKYEQVLGGKGAIHFKTDNRGLFE
YSLTSFSEYGLVLTFVSLDLHQSDFEGNVMTEYEEKFAAKGQPIYRVEAEWRT

Sequences:

>Translated_213_residues
MRMRHKPWADDYLAENSHIVISEPSQYKGKWHSVFGNDNPIHIEVGTGKGQFISGMALQNPDVNYIGIELFKSVIVTAVD
KVKQTEAPNVKLLNINANMLSDVFADGEVDRVYLNFSDPWPKKRHEKRRLTNHAFLKKYEQVLGGKGAIHFKTDNRGLFE
YSLTSFSEYGLVLTFVSLDLHQSDFEGNVMTEYEEKFAAKGQPIYRVEAEWRT
>Mature_213_residues
MRMRHKPWADDYLAENSHIVISEPSQYKGKWHSVFGNDNPIHIEVGTGKGQFISGMALQNPDVNYIGIELFKSVIVTAVD
KVKQTEAPNVKLLNINANMLSDVFADGEVDRVYLNFSDPWPKKRHEKRRLTNHAFLKKYEQVLGGKGAIHFKTDNRGLFE
YSLTSFSEYGLVLTFVSLDLHQSDFEGNVMTEYEEKFAAKGQPIYRVEAEWRT

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=136, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BACLD (Q65G12)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080281.1
- RefSeq:   YP_092695.1
- ProteinModelPortal:   Q65G12
- SMR:   Q65G12
- STRING:   Q65G12
- EnsemblBacteria:   EBBACT00000058276
- EnsemblBacteria:   EBBACT00000059053
- GeneID:   3027940
- GeneID:   3098881
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03141
- KEGG:   bli:BL00042
- NMPDR:   fig|279010.5.peg.113
- eggNOG:   COG0220
- GeneTree:   EBGT00050000000917
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   BLIC279010-1:BLI03141-MONOMER
- BioCyc:   BLIC279010:BL00042-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24431; Mature: 24431

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00213 LIPOCALIN

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMRHKPWADDYLAENSHIVISEPSQYKGKWHSVFGNDNPIHIEVGTGKGQFISGMALQN
CCCCCCCCCCHHHCCCCEEEEECCHHHCCCEEEECCCCCEEEEEEECCCCCEEECEEECC
PDVNYIGIELFKSVIVTAVDKVKQTEAPNVKLLNINANMLSDVFADGEVDRVYLNFSDPW
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCC
PKKRHEKRRLTNHAFLKKYEQVLGGKGAIHFKTDNRGLFEYSLTSFSEYGLVLTFVSLDL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHCCEEEEEEEEEC
HQSDFEGNVMTEYEEKFAAKGQPIYRVEAEWRT
CCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MRMRHKPWADDYLAENSHIVISEPSQYKGKWHSVFGNDNPIHIEVGTGKGQFISGMALQN
CCCCCCCCCCHHHCCCCEEEEECCHHHCCCEEEECCCCCEEEEEEECCCCCEEECEEECC
PDVNYIGIELFKSVIVTAVDKVKQTEAPNVKLLNINANMLSDVFADGEVDRVYLNFSDPW
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHCCCCEEEEEEECCCCC
PKKRHEKRRLTNHAFLKKYEQVLGGKGAIHFKTDNRGLFEYSLTSFSEYGLVLTFVSLDL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHCCEEEEEEEEEC
HQSDFEGNVMTEYEEKFAAKGQPIYRVEAEWRT
CCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA