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The map label for this gene is malS [H]

Identifier: 52786864

GI number: 52786864

Start: 3020492

End: 3022207

Strand: Reverse

Name: malS [H]

Synonym: BLi03139

Alternate gene names: 52786864

Gene position: 3022207-3020492 (Counterclockwise)

Preceding gene: 52786865

Following gene: 52786862

Centisome position: 71.57

GC content: 51.57

Gene sequence:

>1716_bases
TTGAAAGGAAGACGACGGGTGAGCCACAATAAATTAAATGAACAAGTGATCGAGACGCGGTTAAGAGGATTTGAAGTCCT
TGCGACTCCCCTGTTGAACAAAGGGGTTGCCTTCCCTCCGGAGGAAAGGGATGCTCTCGGCTTGACAGGATTGCTTCCTC
CCAAGGTGCTGACGCTTGAAGAACAGGCAAAACGGGCGTATAAACAGTTCCAATCACAGCCTGATGATTTGAGCAAAAAC
GTCTATTTAACGGCGCTTCATGATCGAAATGAAGTGCTGTTCTACAGGCTTCTGAACGACCATATGACAGAAATGCTGCC
GATTGTCTATACCCCGACTGTCGGAACGGCAATACAGCAATACAGCCATGAATACAGAAAGCCGCGGGGGCTGTTTTTGT
CGGTTAATGACCCTGAAGGCATGAAAACGGCATTTGAAAATCTGGCCGCTAAAAGAAGCCGGATCGATTTGATCGTGGCC
ACTGATGCTGAAGGAATTCTCGGAATCGGGGATTGGGGAGTCGGCGGCATCGCCATTTCGATCGGAAAGCTTGCTGTTTA
TACTGCAGCTGCGGGCATCGATCCTAACAGGGTCCTGCCAGTAGTCATTGATGCGGGGACGAACCAGGAAAGGCTTCTGA
ACGATCCGCTTTATGTCGGGAATCAGCATTCGAGGATCAGGGGCGAGCGTTATGAATCGTTTATTGATCAATACGTTCAA
ATCGCAACCGAGACGTTTCCGGGCGTTTTGCTGCACTGGGAAGATTTCGGAACGCAGAACGCGCGTCAGATTTTGAAAAA
ATACAAAGACAAAATTTGCACCTTTAATGATGACATTCAAGGCACAGGAGCTGTTACCCTCGCAGCGGTGCTTGCGGCCG
TTAAAATATCAAAAATGCCGCTGAAGGATCATAAAGTGGTCATATTTGGAGCGGGGACGGCCGGAATCGGCAATGCTGAG
CAAATTCGCGCCGCACTCGTTCAAGAGGGAGTGTCGGAAGAGGAATCATACCGCCGTTTTTGGTGTGTTGATCGGAACGG
TTTGCTGACAGATGATATGAACGACTTGCAGGACATTCAACAACCGTATGCAAGGCCTGCTGACGAGGTAAAAGACTTCA
GCCGGAACGGCCCGGGGGATGCAATTGATCTGCTCGAGGTGGTTAAAAGAGTGCAGCCTACCATTTTGATTGGAACCTCG
ACGGTATCGGGCGCATTTACAGAGGAAATTGTAAAAGAAATGGCAAAGCATGTGAAACGGCCGGCTATATTCCCGATGTC
CAATCCGACGCCGTTATCTGAAGCAAGGCCTGAAGATTTGATCAATTGGACGGAAGGGCGGGCGCTCGTCGCCACAGGAA
GCCCGTTTGCGCCTGTGGAATACAGCGGCACCCGTTATGCGATCGGCCAGGCGAATAATGCCTTTGTATTCCCCGGCCTT
GGGCTCGGAACGCTCGTATCAAAAGCAAGACTGATTACGGACAACATGTTTTACGCCAGCGCGGCTGCGATCGCGGGTAT
GGTTGATACGGGCCGGCCCGGCGCCGCGCTTCTTCCGGAAGTTGAGGATTTGCGCACGGTTTCCGCAACCGTGGCAACGG
CTGTCATCAAAGCAGCCATAGAAGACGGAGTTGCAGAAAACGAGCCGGACGATGTGATTCAGGCGGTGCAGGATGCGATG
TGGCATCCGGTCTACCGGCCGATTCGCGCCATATAA

Upstream 100 bases:

>100_bases
TGAAAACGGCGATATTGCAGAGTCAGTAAAAAGAGAAAGCCGCTAGCTATTTTGGTGAATGGTTTGCTTTGTTGTTATCC
ATTCTAAGGGTAACTAACTT

Downstream 100 bases:

>100_bases
AAAGATGCGGCCCTGATGGCCGCATCTTTTTTGATGCTATGCTGCTGTATCAATTACATCTACGATCATGCCTGTTTTTG
CATCAACCATGACTTCGTAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKGRRRVSHNKLNEQVIETRLRGFEVLATPLLNKGVAFPPEERDALGLTGLLPPKVLTLEEQAKRAYKQFQSQPDDLSKN
VYLTALHDRNEVLFYRLLNDHMTEMLPIVYTPTVGTAIQQYSHEYRKPRGLFLSVNDPEGMKTAFENLAAKRSRIDLIVA
TDAEGILGIGDWGVGGIAISIGKLAVYTAAAGIDPNRVLPVVIDAGTNQERLLNDPLYVGNQHSRIRGERYESFIDQYVQ
IATETFPGVLLHWEDFGTQNARQILKKYKDKICTFNDDIQGTGAVTLAAVLAAVKISKMPLKDHKVVIFGAGTAGIGNAE
QIRAALVQEGVSEEESYRRFWCVDRNGLLTDDMNDLQDIQQPYARPADEVKDFSRNGPGDAIDLLEVVKRVQPTILIGTS
TVSGAFTEEIVKEMAKHVKRPAIFPMSNPTPLSEARPEDLINWTEGRALVATGSPFAPVEYSGTRYAIGQANNAFVFPGL
GLGTLVSKARLITDNMFYASAAAIAGMVDTGRPGAALLPEVEDLRTVSATVATAVIKAAIEDGVAENEPDDVIQAVQDAM
WHPVYRPIRAI

Sequences:

>Translated_571_residues
MKGRRRVSHNKLNEQVIETRLRGFEVLATPLLNKGVAFPPEERDALGLTGLLPPKVLTLEEQAKRAYKQFQSQPDDLSKN
VYLTALHDRNEVLFYRLLNDHMTEMLPIVYTPTVGTAIQQYSHEYRKPRGLFLSVNDPEGMKTAFENLAAKRSRIDLIVA
TDAEGILGIGDWGVGGIAISIGKLAVYTAAAGIDPNRVLPVVIDAGTNQERLLNDPLYVGNQHSRIRGERYESFIDQYVQ
IATETFPGVLLHWEDFGTQNARQILKKYKDKICTFNDDIQGTGAVTLAAVLAAVKISKMPLKDHKVVIFGAGTAGIGNAE
QIRAALVQEGVSEEESYRRFWCVDRNGLLTDDMNDLQDIQQPYARPADEVKDFSRNGPGDAIDLLEVVKRVQPTILIGTS
TVSGAFTEEIVKEMAKHVKRPAIFPMSNPTPLSEARPEDLINWTEGRALVATGSPFAPVEYSGTRYAIGQANNAFVFPGL
GLGTLVSKARLITDNMFYASAAAIAGMVDTGRPGAALLPEVEDLRTVSATVATAVIKAAIEDGVAENEPDDVIQAVQDAM
WHPVYRPIRAI
>Mature_571_residues
MKGRRRVSHNKLNEQVIETRLRGFEVLATPLLNKGVAFPPEERDALGLTGLLPPKVLTLEEQAKRAYKQFQSQPDDLSKN
VYLTALHDRNEVLFYRLLNDHMTEMLPIVYTPTVGTAIQQYSHEYRKPRGLFLSVNDPEGMKTAFENLAAKRSRIDLIVA
TDAEGILGIGDWGVGGIAISIGKLAVYTAAAGIDPNRVLPVVIDAGTNQERLLNDPLYVGNQHSRIRGERYESFIDQYVQ
IATETFPGVLLHWEDFGTQNARQILKKYKDKICTFNDDIQGTGAVTLAAVLAAVKISKMPLKDHKVVIFGAGTAGIGNAE
QIRAALVQEGVSEEESYRRFWCVDRNGLLTDDMNDLQDIQQPYARPADEVKDFSRNGPGDAIDLLEVVKRVQPTILIGTS
TVSGAFTEEIVKEMAKHVKRPAIFPMSNPTPLSEARPEDLINWTEGRALVATGSPFAPVEYSGTRYAIGQANNAFVFPGL
GLGTLVSKARLITDNMFYASAAAIAGMVDTGRPGAALLPEVEDLRTVSATVATAVIKAAIEDGVAENEPDDVIQAVQDAM
WHPVYRPIRAI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=549, Percent_Identity=40.9836065573771, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI62420880, Length=549, Percent_Identity=40.9836065573771, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI239049447, Length=549, Percent_Identity=40.9836065573771, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4505143, Length=556, Percent_Identity=40.1079136690648, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4505145, Length=556, Percent_Identity=38.1294964028777, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI270265879, Length=467, Percent_Identity=41.1134903640257, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87081919, Length=546, Percent_Identity=46.1538461538462, Blast_Score=480, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17537199, Length=550, Percent_Identity=41.8181818181818, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322823, Length=578, Percent_Identity=44.1176470588235, Blast_Score=494, Evalue=1e-140,
Organism=Drosophila melanogaster, GI21356279, Length=566, Percent_Identity=38.6925795053004, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281362672, Length=566, Percent_Identity=38.6925795053004, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI281362674, Length=566, Percent_Identity=38.6925795053004, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24646386, Length=535, Percent_Identity=38.6915887850467, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24646388, Length=535, Percent_Identity=38.6915887850467, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281363505, Length=542, Percent_Identity=35.239852398524, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78707236, Length=542, Percent_Identity=35.239852398524, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281363503, Length=540, Percent_Identity=35, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707232, Length=517, Percent_Identity=35.9767891682785, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707242, Length=549, Percent_Identity=36.247723132969, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707238, Length=549, Percent_Identity=36.247723132969, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707240, Length=525, Percent_Identity=36.7619047619048, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI19922384, Length=560, Percent_Identity=26.0714285714286, Blast_Score=247, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 62648; Mature: 62648

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRRRVSHNKLNEQVIETRLRGFEVLATPLLNKGVAFPPEERDALGLTGLLPPKVLTLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEHHH
EQAKRAYKQFQSQPDDLSKNVYLTALHDRNEVLFYRLLNDHMTEMLPIVYTPTVGTAIQQ
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCEEECCCHHHHHHH
YSHEYRKPRGLFLSVNDPEGMKTAFENLAAKRSRIDLIVATDAEGILGIGDWGVGGIAIS
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCEEEE
IGKLAVYTAAAGIDPNRVLPVVIDAGTNQERLLNDPLYVGNQHSRIRGERYESFIDQYVQ
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHH
IATETFPGVLLHWEDFGTQNARQILKKYKDKICTFNDDIQGTGAVTLAAVLAAVKISKMP
HHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
LKDHKVVIFGAGTAGIGNAEQIRAALVQEGVSEEESYRRFWCVDRNGLLTDDMNDLQDIQ
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEECCCCCCCCCHHHHHHHH
QPYARPADEVKDFSRNGPGDAIDLLEVVKRVQPTILIGTSTVSGAFTEEIVKEMAKHVKR
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
PAIFPMSNPTPLSEARPEDLINWTEGRALVATGSPFAPVEYSGTRYAIGQANNAFVFPGL
CCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCEECCCEEEECCCCCEEEECCC
GLGTLVSKARLITDNMFYASAAAIAGMVDTGRPGAALLPEVEDLRTVSATVATAVIKAAI
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EDGVAENEPDDVIQAVQDAMWHPVYRPIRAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGRRRVSHNKLNEQVIETRLRGFEVLATPLLNKGVAFPPEERDALGLTGLLPPKVLTLE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEHHH
EQAKRAYKQFQSQPDDLSKNVYLTALHDRNEVLFYRLLNDHMTEMLPIVYTPTVGTAIQQ
HHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCEEECCCHHHHHHH
YSHEYRKPRGLFLSVNDPEGMKTAFENLAAKRSRIDLIVATDAEGILGIGDWGVGGIAIS
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCEEEE
IGKLAVYTAAAGIDPNRVLPVVIDAGTNQERLLNDPLYVGNQHSRIRGERYESFIDQYVQ
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHH
IATETFPGVLLHWEDFGTQNARQILKKYKDKICTFNDDIQGTGAVTLAAVLAAVKISKMP
HHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
LKDHKVVIFGAGTAGIGNAEQIRAALVQEGVSEEESYRRFWCVDRNGLLTDDMNDLQDIQ
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEECCCCCCCCCHHHHHHHH
QPYARPADEVKDFSRNGPGDAIDLLEVVKRVQPTILIGTSTVSGAFTEEIVKEMAKHVKR
HHHCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
PAIFPMSNPTPLSEARPEDLINWTEGRALVATGSPFAPVEYSGTRYAIGQANNAFVFPGL
CCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCEECCCEEEECCCCCEEEECCC
GLGTLVSKARLITDNMFYASAAAIAGMVDTGRPGAALLPEVEDLRTVSATVATAVIKAAI
CHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EDGVAENEPDDVIQAVQDAMWHPVYRPIRAI
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]