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The map label for this gene is yrkH [H]

Identifier: 52786520

GI number: 52786520

Start: 2674360

End: 2675793

Strand: Reverse

Name: yrkH [H]

Synonym: BLi02785

Alternate gene names: 52786520

Gene position: 2675793-2674360 (Counterclockwise)

Preceding gene: 52786521

Following gene: 52786519

Centisome position: 63.37

GC content: 49.23

Gene sequence:

>1434_bases
ATGTTATTGCGCTATTTTTACGATAAAAAACTGGCCCATGCATCCTATCTGGTTGGCTGCCAAAAAACAGGGGAAGCCAT
TGTGATTGACCCCGGCCGCAATCTAAAACCATATTTGCAAGCAGCCAAGGAAGAAGGGCTAAACATCACAGCGGCCGCAG
AAACCCATATTCACGCCGACTTTGTTTCCGGTGCCCGCGAACTCGGCGCTACACATCAAGCGAAGCTGTATCTTTCCGAT
GAAGGAGACGCAAATTGGAAATACCAATATGTCGATGAATCGAAACATCAACTTGTGAAAGACGGCGATCGCTTTTCCAT
TGGTAACCTGATGTTTGAGGTTATGCATACTCCCGGCCATACCCCGGAAAGCATTTCTTTCCTGTTGACGGACGGCGGAG
GAAATGCGGATCGTCCAATCGGCATCTTTACTGGAGATTTCGTCTTTGCAGGCGACATCGGCAGACCCGATTTGCTTGAA
AAGGCGGCGGGGATTAAGGGAACGTCAGAGTCGGGGGCAAGAGCGATGTTCAAGTCCCTCATGAAGTTTAAACAGCTCCC
GGATTACTTGCAGGTTTGGCCGGCTCACGGCGCAGGGAGCGCTTGCGGCAAGGCATTGGGAGCCATTCCAACTACGACTG
TCGGCTACGAAAAGATGTTTAACTGGGCAATGGCTTACACCGATGAAGAGGAATTCGTAAATGCCTTGCTTGATGGACAA
CCAGAACCGCCGAAGTATTTTGCTGAGATGAAACGAGTGAATAAAGAAGGCCCTGCATTGCTCAAAGATCTTTCTGCCGT
TTTAGAGATTATGTCGATTGAACAAGTGAAAGATTTGCAGGGGAAAGAGCAAATTGTGGATACCCGCTCCTCCCGGGAAT
TTGCTGAAGGGCACCTCAAAGGCACGATCAACATTCCATTCAACAAGGGGTTTACAAACTGGGCAGGCTGGCTCATCGAC
TACAATCGTCCTGTGTACTTAATCGCGAATCCGGCAGAAGTGAACGAACTCCTGGAAGCGCTGCGCTCCGTAGGCATTGA
CAACGCGGCAGGGTTTATCGATCGTGACAAAATCTTGGCAGACGGTGCTTTTTCACTTGAATCGTACGCTGAGATCACAC
CACGTGAGATCGCAAAATTAGTGGAAGACGGCTTTGTGCAAGTGCTTGATGTTCGCAATCTGACTGAATGGCAGGAAGGT
CACATTCCGAATGCGCAACACATCATGCTGGGAACGCTCCCAGAACGGCTCGATGAAATTAGAAAAGACTGTCCGATTCT
TGTGCAATGCCATTCCGGAGCCCGATCTGCAATAGGAGCCAGCATCCTGCAAGCTAACGGTAGTTTCAAACAAGTATTGA
GTTTAAGCGGCGGCATTGTCCAATGGCAGAAAGACGGGCTTGCAATTTCAACCCCTTGCAGCGAATTAAAATGA

Upstream 100 bases:

>100_bases
GAATGGCATGTTAAAAGGACTGATGTAAGACAGGACAAAGCATGATGCGAACTCAAAAAAGGATATATTACGATAAATGT
TTCAAGAGGGGGAATACAAC

Downstream 100 bases:

>100_bases
AAGAAAATTTCTCTGCTCAAGCGCATGGAATAAAAAGTCAAGAGGCCGCTATGTCTTACTCCCGGCCTCTTGACTGATAG
TGTTTCCGCTTTTCCTTCTC

Product: hypothetical protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLLRYFYDKKLAHASYLVGCQKTGEAIVIDPGRNLKPYLQAAKEEGLNITAAAETHIHADFVSGARELGATHQAKLYLSD
EGDANWKYQYVDESKHQLVKDGDRFSIGNLMFEVMHTPGHTPESISFLLTDGGGNADRPIGIFTGDFVFAGDIGRPDLLE
KAAGIKGTSESGARAMFKSLMKFKQLPDYLQVWPAHGAGSACGKALGAIPTTTVGYEKMFNWAMAYTDEEEFVNALLDGQ
PEPPKYFAEMKRVNKEGPALLKDLSAVLEIMSIEQVKDLQGKEQIVDTRSSREFAEGHLKGTINIPFNKGFTNWAGWLID
YNRPVYLIANPAEVNELLEALRSVGIDNAAGFIDRDKILADGAFSLESYAEITPREIAKLVEDGFVQVLDVRNLTEWQEG
HIPNAQHIMLGTLPERLDEIRKDCPILVQCHSGARSAIGASILQANGSFKQVLSLSGGIVQWQKDGLAISTPCSELK

Sequences:

>Translated_477_residues
MLLRYFYDKKLAHASYLVGCQKTGEAIVIDPGRNLKPYLQAAKEEGLNITAAAETHIHADFVSGARELGATHQAKLYLSD
EGDANWKYQYVDESKHQLVKDGDRFSIGNLMFEVMHTPGHTPESISFLLTDGGGNADRPIGIFTGDFVFAGDIGRPDLLE
KAAGIKGTSESGARAMFKSLMKFKQLPDYLQVWPAHGAGSACGKALGAIPTTTVGYEKMFNWAMAYTDEEEFVNALLDGQ
PEPPKYFAEMKRVNKEGPALLKDLSAVLEIMSIEQVKDLQGKEQIVDTRSSREFAEGHLKGTINIPFNKGFTNWAGWLID
YNRPVYLIANPAEVNELLEALRSVGIDNAAGFIDRDKILADGAFSLESYAEITPREIAKLVEDGFVQVLDVRNLTEWQEG
HIPNAQHIMLGTLPERLDEIRKDCPILVQCHSGARSAIGASILQANGSFKQVLSLSGGIVQWQKDGLAISTPCSELK
>Mature_477_residues
MLLRYFYDKKLAHASYLVGCQKTGEAIVIDPGRNLKPYLQAAKEEGLNITAAAETHIHADFVSGARELGATHQAKLYLSD
EGDANWKYQYVDESKHQLVKDGDRFSIGNLMFEVMHTPGHTPESISFLLTDGGGNADRPIGIFTGDFVFAGDIGRPDLLE
KAAGIKGTSESGARAMFKSLMKFKQLPDYLQVWPAHGAGSACGKALGAIPTTTVGYEKMFNWAMAYTDEEEFVNALLDGQ
PEPPKYFAEMKRVNKEGPALLKDLSAVLEIMSIEQVKDLQGKEQIVDTRSSREFAEGHLKGTINIPFNKGFTNWAGWLID
YNRPVYLIANPAEVNELLEALRSVGIDNAAGFIDRDKILADGAFSLESYAEITPREIAKLVEDGFVQVLDVRNLTEWQEG
HIPNAQHIMLGTLPERLDEIRKDCPILVQCHSGARSAIGASILQANGSFKQVLSLSGGIVQWQKDGLAISTPCSELK

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=235, Percent_Identity=26.3829787234043, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1787158, Length=219, Percent_Identity=27.3972602739726, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538952, Length=267, Percent_Identity=25.4681647940075, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330176, Length=259, Percent_Identity=25.4826254826255, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 52407; Mature: 52407

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRYFYDKKLAHASYLVGCQKTGEAIVIDPGRNLKPYLQAAKEEGLNITAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
FVSGARELGATHQAKLYLSDEGDANWKYQYVDESKHQLVKDGDRFSIGNLMFEVMHTPGH
HHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHCCCCCEEHHHHHHHHHHCCCC
TPESISFLLTDGGGNADRPIGIFTGDFVFAGDIGRPDLLEKAAGIKGTSESGARAMFKSL
CCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
MKFKQLPDYLQVWPAHGAGSACGKALGAIPTTTVGYEKMFNWAMAYTDEEEFVNALLDGQ
HHHHHCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCC
PEPPKYFAEMKRVNKEGPALLKDLSAVLEIMSIEQVKDLQGKEQIVDTRSSREFAEGHLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCE
GTINIPFNKGFTNWAGWLIDYNRPVYLIANPAEVNELLEALRSVGIDNAAGFIDRDKILA
EEEECCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
DGAFSLESYAEITPREIAKLVEDGFVQVLDVRNLTEWQEGHIPNAQHIMLGTLPERLDEI
CCCCCHHHHHCCCHHHHHHHHHCCCEEEHHHCCCHHHHHCCCCCCCEEEECCHHHHHHHH
RKDCPILVQCHSGARSAIGASILQANGSFKQVLSLSGGIVQWQKDGLAISTPCSELK
HCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCEEEECCHHHCC
>Mature Secondary Structure
MLLRYFYDKKLAHASYLVGCQKTGEAIVIDPGRNLKPYLQAAKEEGLNITAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHH
FVSGARELGATHQAKLYLSDEGDANWKYQYVDESKHQLVKDGDRFSIGNLMFEVMHTPGH
HHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCHHHHHHCCCCCEEHHHHHHHHHHCCCC
TPESISFLLTDGGGNADRPIGIFTGDFVFAGDIGRPDLLEKAAGIKGTSESGARAMFKSL
CCCCEEEEEECCCCCCCCCEEEEECCEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHH
MKFKQLPDYLQVWPAHGAGSACGKALGAIPTTTVGYEKMFNWAMAYTDEEEFVNALLDGQ
HHHHHCCHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCC
PEPPKYFAEMKRVNKEGPALLKDLSAVLEIMSIEQVKDLQGKEQIVDTRSSREFAEGHLK
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCE
GTINIPFNKGFTNWAGWLIDYNRPVYLIANPAEVNELLEALRSVGIDNAAGFIDRDKILA
EEEECCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
DGAFSLESYAEITPREIAKLVEDGFVQVLDVRNLTEWQEGHIPNAQHIMLGTLPERLDEI
CCCCCHHHHHCCCHHHHHHHHHCCCEEEHHHCCCHHHHHCCCCCCCEEEECCHHHHHHHH
RKDCPILVQCHSGARSAIGASILQANGSFKQVLSLSGGIVQWQKDGLAISTPCSELK
HCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]