Click here to switch to the map view.

The map label for this gene is alaXL [H]

Identifier: 52786509

GI number: 52786509

Start: 2665677

End: 2666909

Strand: Reverse

Name: alaXL [H]

Synonym: BLi02774

Alternate gene names: 52786509

Gene position: 2666909-2665677 (Counterclockwise)

Preceding gene: 52786510

Following gene: 52786506

Centisome position: 63.16

GC content: 38.69

Gene sequence:

>1233_bases
ATGAGTCCGAATCATCACAAACTATTTTACAAAGATCCCTATCTAAAAACGTTTGAAACGAAAATCTTGAAACAACAACA
GGACAAAAAAGGTGATGTTTATATTATCCTAGAGGAAACAGCCTTTTATCCAACAGGCGGGGGCCAGCCTCATGATACAG
GAACATTGAACGGAGTCAAAGTTTTAAACGTTGATGAGATAGATGGAGAAATTCGTCATTATGTAGAAAGGCCTTTGCCG
CATTTAAATGATCAGGTTGTTGGGGTTATTGATTGGGAGCGGCGTTTTGATCACATGCAACAGCACACCGGGCAGCATAT
TTTGTCGGCGGCATTCGCGCAGCTGTATGATCTTCATACAGTTGGATTCCATCTTGGAAATGATACGGTTACCATTGACC
TTGATGTATCAGAATTAACAGAGGAAATGGCAGGCAAAGCAGAGAAGCTTGCAAACGAGATCATAACGGAAAATAGGTCC
ATAGTCGCAAAGTGGGTAGAATATGATGACTTAGTAAATTATCCGATTCGTAAACTCCCATCTGTAAAAGAAAATATTCG
TCTTGTCATGATTGATGAATTTGATTACAACGGATGCGGCGGAACACACCCGAGCGCTACAGGAGAAGTCGGAGCAATAA
AAGTATTGAATTGGGAAAGACAGAGGAAAAAAATTCGTCTGTATTTTGTATGCGGTAATCGGGTCATAACCCAATTCGAT
CAAAAACAAAAAGCAATCGTTGAATTGACTGGATTACTTAATCGTCCTGAAGGGGAATTAAGCGAAGCTATCAAACGGTT
GCTTGAAAATGTAAAGAAGCTAGACAAAGCGCTTCTAGAATCTTATGAAAAAAACCTTCAGTATGAAGCCAATGAATTAT
TAGCAAAAAGCGCAATTCAAAATGATACTGTAATGGTGATAGAAACGTTTTCAGATCGATCGATTCAAGAACTTCAAAAG
CTAGCAAAAATGATTGCTGATCGAGAAAAACGGGCCCTTATTTTTTTCTTGTCAGAGAACGATCATCGCTTCCAATTGGT
TTGCGGGCGAGGTGAAGCAAATAAAATGAACATGAGAGACCTAATGCAAGAAGCTCTTTCCCTTATTGATGGAAAAGGTG
GGGGAAATAAAATGTTCGCTCAAGGTGGAGGGACTGCTTCATTCACAAAAGAGAAATTTATGAAAAATGTTATGAACATT
ATAGCAATACAAATAAACTCAGAAGAAAATTAA

Upstream 100 bases:

>100_bases
ACGATAAAGCCTATTTAGTCGATAATGGAGAGGTTGTAGATGAATTAAATATTGAAGCTAGAGGAAGACTTCAATAATTT
AAAAAATAAGGGGAGATGGC

Downstream 100 bases:

>100_bases
AAGTAACTTCAACAGGCCTCTGTAATTGGATAACAAGCTTTATTAAGGGCCTGGCAGAAAGAAATGGAAGCCGGTGTTTT
AGGCCGGCTTCCATGACATC

Product: hypothetical protein

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSPNHHKLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVKVLNVDEIDGEIRHYVERPLP
HLNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHTVGFHLGNDTVTIDLDVSELTEEMAGKAEKLANEIITENRS
IVAKWVEYDDLVNYPIRKLPSVKENIRLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFD
QKQKAIVELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQNDTVMVIETFSDRSIQELQK
LAKMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRDLMQEALSLIDGKGGGNKMFAQGGGTASFTKEKFMKNVMNI
IAIQINSEEN

Sequences:

>Translated_410_residues
MSPNHHKLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVKVLNVDEIDGEIRHYVERPLP
HLNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHTVGFHLGNDTVTIDLDVSELTEEMAGKAEKLANEIITENRS
IVAKWVEYDDLVNYPIRKLPSVKENIRLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFD
QKQKAIVELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQNDTVMVIETFSDRSIQELQK
LAKMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRDLMQEALSLIDGKGGGNKMFAQGGGTASFTKEKFMKNVMNI
IAIQINSEEN
>Mature_409_residues
SPNHHKLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVKVLNVDEIDGEIRHYVERPLPH
LNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHTVGFHLGNDTVTIDLDVSELTEEMAGKAEKLANEIITENRSI
VAKWVEYDDLVNYPIRKLPSVKENIRLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFDQ
KQKAIVELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQNDTVMVIETFSDRSIQELQKL
AKMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRDLMQEALSLIDGKGGGNKMFAQGGGTASFTKEKFMKNVMNII
AIQINSEEN

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=358, Percent_Identity=31.2849162011173, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI217416402, Length=358, Percent_Identity=31.2849162011173, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1789048, Length=368, Percent_Identity=22.8260869565217, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324288, Length=291, Percent_Identity=28.8659793814433, Blast_Score=106, Evalue=7e-24,
Organism=Drosophila melanogaster, GI18543305, Length=304, Percent_Identity=32.2368421052632, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 46731; Mature: 46600

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNHHKLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVK
CCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCCCCCCCCCCCCCEE
VLNVDEIDGEIRHYVERPLPHLNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHT
EEEEHHCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFHLGNDTVTIDLDVSELTEEMAGKAEKLANEIITENRSIVAKWVEYDDLVNYPIRKLP
HEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCC
SVKENIRLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFD
CHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
QKQKAIVELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
NDTVMVIETFSDRSIQELQKLAKMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRD
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHH
LMQEALSLIDGKGGGNKMFAQGGGTASFTKEKFMKNVMNIIAIQINSEEN
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCC
>Mature Secondary Structure 
SPNHHKLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVK
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCEECCCCCCCCCCCCCCCCEE
VLNVDEIDGEIRHYVERPLPHLNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHT
EEEEHHCCHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGFHLGNDTVTIDLDVSELTEEMAGKAEKLANEIITENRSIVAKWVEYDDLVNYPIRKLP
HEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCC
SVKENIRLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFD
CHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
QKQKAIVELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
NDTVMVIETFSDRSIQELQKLAKMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRD
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCHHH
LMQEALSLIDGKGGGNKMFAQGGGTASFTKEKFMKNVMNIIAIQINSEEN
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]