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The map label for this gene is spoIIIC [H]

Identifier: 52786504

GI number: 52786504

Start: 2662574

End: 2663299

Strand: Direct

Name: spoIIIC [H]

Synonym: BLi02769

Alternate gene names: 52786504

Gene position: 2662574-2663299 (Clockwise)

Preceding gene: 52786502

Following gene: 52786507

Centisome position: 63.05

GC content: 47.52

Gene sequence:

>726_bases
ATGACAGGTGTTTTTGCAGCGCTCGGTTATGTCATCAAGGAACTCGTTTTTTTAGTATCATATGTGAAAAACAATGCCTT
TCCACAACCGCTGTCAAGCAGTGACGAAAAAAAGTACTTGGAGCTGATGGCCCAGGGGGATGAACACGCGAGAAACATGC
TGATCGAACATAACCTCCGGCTGGTCGCCCACATCGTCAAAAAATTCGAAAACACAGGCGAGGATGCAGAAGACCTGATT
TCCATCGGAACGATCGGGCTGATCAAAGCAATCGAAAGCTATTCAGCCGGAAAAGGAACTAAGCTCGCCACTTATGCGGC
GCGTTGTATAGAAAACGAGATCCTCATGCATTTGCGCGCATTGAAAAAAACGAAAAAAGACGTCTCACTCCATGACCCGA
TCGGCCAGGACAAAGAAGGCAACGAAATCAGCCTCATTGATGTCCTCAAATCGGAAAACGAAGACGTCATCGACACCATC
CAGCTCAACATGGAGCTTGAAAAAGTGAAAAAATACATCGACATCCTGGACGGCAGAGAAAAAGAAGTCATCGTCGGCCG
CTTCGGCCTTGATTTGAAGAAAGAGAAAACACAGCGCGAAATTGCGAAGGAGCTTGGGATTTCGCGGAGCTATGTGTCGC
GGATTGAGAAACGGGCGCTGATGAAGATGTTTCATGAGTTTTATCGGGCGGAGAAGGAGAAAAGGAAGAAGGCGAAGGGG
AAGTGA

Upstream 100 bases:

>100_bases
CAGTCCGGCAATTTTGCCGGGTACAGACACAGACATCCCCCCTCCCACATACATTTACATATAGGCTTCTGCCTACATAC
ATTTTGTGGAGGTGCCGTTA

Downstream 100 bases:

>100_bases
GATCGATGAAGAGGCTCGGAATTATATAAACCCGGCCTCTTTTTATGCGAGCTAATTTGGCTGGGTAACGGGCGCGTCTT
CTGCTTGATGAAGTTTCCCC

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: Sigma-27; Stage IV sporulation protein CB [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTGVFAALGYVIKELVFLVSYVKNNAFPQPLSSSDEKKYLELMAQGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLI
SIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTI
QLNMELEKVKKYIDILDGREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKG
K

Sequences:

>Translated_241_residues
MTGVFAALGYVIKELVFLVSYVKNNAFPQPLSSSDEKKYLELMAQGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLI
SIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTI
QLNMELEKVKKYIDILDGREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKG
K
>Mature_240_residues
TGVFAALGYVIKELVFLVSYVKNNAFPQPLSSSDEKKYLELMAQGDEHARNMLIEHNLRLVAHIVKKFENTGEDAEDLIS
IGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRALKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQ
LNMELEKVKKYIDILDGREKEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKGK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the mother cell [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=245, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27456; Mature: 27325

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVFAALGYVIKELVFLVSYVKNNAFPQPLSSSDEKKYLELMAQGDEHARNMLIEHNLR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LVAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQLNMELEKVKKYIDILDGRE
HHHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKG
CEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
K
C
>Mature Secondary Structure 
TGVFAALGYVIKELVFLVSYVKNNAFPQPLSSSDEKKYLELMAQGDEHARNMLIEHNLR
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LVAHIVKKFENTGEDAEDLISIGTIGLIKAIESYSAGKGTKLATYAARCIENEILMHLRA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LKKTKKDVSLHDPIGQDKEGNEISLIDVLKSENEDVIDTIQLNMELEKVKKYIDILDGRE
HHHHHHCCCCCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KEVIVGRFGLDLKKEKTQREIAKELGISRSYVSRIEKRALMKMFHEFYRAEKEKRKKAKG
CEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2536191; 2105293; 2841290; 3125151; 8969508; 9384377; 2492118 [H]