Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 52785723

GI number: 52785723

Start: 1921031

End: 1923133

Strand: Direct

Name: nrdE [H]

Synonym: BLi01966

Alternate gene names: 52785723

Gene position: 1921031-1923133 (Clockwise)

Preceding gene: 52785722

Following gene: 52785724

Centisome position: 45.49

GC content: 44.7

Gene sequence:

>2103_bases
TTGTCACAAAATCAAGTGCCAAAATGGATCCAGTTAAACAATGAGATCATGATCCAAAAAGACGGGAAATTCCAGTTTGA
TAAAGATAAAGAAGCTGTACATAGTTATTTTGTAGACTATATCAACCAAAATACGGTATTTTTCCATGATCTGAAAGAGA
AAATCGATTACTTAATCAAGAATGACTACTATGAAGAAGAGTTTTTAAGCCGCTACACGTTTGAAGAAATCAAAGAAGTG
TTCAAAGAGGCTTACGCCAAAAAATTCAGATTCCCGTCCTTTATGAGCGCTTTTAAGTTTTACAATGATTATGCGCTGAA
GACAAACGACAAGAAAAAAATTCTTGAACGCTATGAAGACAGAATCTCTATCGTAGCTTTATTTTTTGCAAACGGGGATA
AGGAGAAGGCGAAGGAATTCGTCAGAATGATGATCAACCAGGAGTATCAGCCGAGCACGCCGACGTTCTTAAATGCCGGC
CGCAAACGCCGCGGAGAGCTGGTCAGCTGTTTCCTCTTGGAAGTCAATGATTCCTTGAACGACATTTCGAGAGCGATTGA
CATTTCGATGCAGCTGTCAAAGCTCGGCGGAGGCGTATCTTTAAACCTTTCAAAACTGCGTGCGAAAGGCGAAGCGATCA
AAGATGTCGAAAACGCGACAAAAGGTGTCGTCGGCGTGATGAAGCTTCTTGACAACGCATTCCGCTACGCTGACCAAATG
GGGCAAAGACAAGGCTCAGGCGCTGCGTACTTAAACATTTTCCACCGCGACATCAACGATTTCCTCGATACGAAGAAGAT
TTCGGCTGACGAGGATGTCCGGGTGAAAACATTGTCCATCGGGGTTGTCATTCCAGATAAATTCATCGAGCTTGCAAGAG
AGGATAAAGCGGCATACGTCTTCTATCCGCACACCGTTTATAAAGCGTACGGCGAGCACCTCGACGAAATGGATATGAAC
GAGATGTACGACCGGCTTGTGGAAGATCCGCGCGTCAAAAAAGAAAAAATCCACCCGCGCAAGATGCTCGAAAAGCTTGC
GATGTTAAGATCGGAATCCGGGTATCCGTACATTATGTTCCAGGACAACGTCAACAACGTGCATGCGAACAATCATATTT
CAAAAGTTAAATTTTCCAACCTTTGCTCGGAGGTTCTGCAGGCTTCACAAGTTTCATCATACACAGACTACGGTCAAGAA
GATGAGATCGGCCTTGACATCTCCTGCAACCTTGGTTCATTGAACATTTTGAACGTCATGAAAAACAAATCGCTCGAGCA
GACAGTTAAGCTTGCAACCGATTCGCTGACGCACGTTTCTGAAACGACAAACATTACAAACGCACCGGCTGTCAAAAAAG
CGAACAAAGCGATGAAATCCATCGGGCTCGGAGCGATGAACCTGCACGGATTCTTGGCACAGAACAATATCGCCTACGAA
AGTGAGGAAGCGAGGGACTTCGCGAACACTTTCTTTATGATGATGAACTTCTACTCGATTGAACGATCCGCCGAAATCGC
CAAAGAAAAAGGTGAGACGTACCATGGGTACGAAGGTTCAGCTTACGCAACGGGTGAATACTTCGAAAAGTATGTTACAC
AAGATTTCTCGCCTAAATTCGAAAAAGTGGCCAAGCTTTTTGAAGGCATGCACATCCCGACAAAAGAAGACTGGAAGCAG
CTGAAACAGTTTGTCAAAGAACACGGATTGTACCACAGCTACCGGCTGTGCATCGCACCGACCGGATCCATTTCATATGT
ACAGTCAAGCACAGCATCGGTTATGCCGATCATGGAGCGGATTGAAGAAAGAACATACGGCAACTCCAAGACGTATTATC
CAATGCCTGGACTTGCGCCGAGCAACTGGTTCTACTATAAGGAAGCGTATGATATGGACATGTTCAGGGTTGTCGACATG
ATCTCGACGATTCAGCAGCACGTTGACCAAGGCATAAGCTTTACATTGTTCCTGAAAGATACAATGACGACACGCGATTT
GAACCGGATCGATCTTTATGCCCACCATAAAGGAATCAAAACACTTTACTATGCAAGAACGAAGGATACAGGGCAAGAAG
GCTGCCTTTCTTGTGTCGTTTGA

Upstream 100 bases:

>100_bases
GAAAAAATCTCGAAGCAGTATCAGGTTCCGATTCTCGGCAAATTTGAGCTCAGCGGGACGGCCAAAGACGTTGAACTGTT
TACTCAGGAGGTAGAAAGAG

Downstream 100 bases:

>100_bases
TTAAAGGAGAGTATGCGAAAGTGACGAAAATTTACGATGCAGCAAACTGGTCAAAGCATGAAGATGATTTTACCCAAATG
TTTTACAACCAAAATGTAAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHDLKEKIDYLIKNDYYEEEFLSRYTFEEIKEV
FKEAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDKEKAKEFVRMMINQEYQPSTPTFLNAG
RKRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFIELAREDKAAYVFYPHTVYKAYGEHLDEMDMN
EMYDRLVEDPRVKKEKIHPRKMLEKLAMLRSESGYPYIMFQDNVNNVHANNHISKVKFSNLCSEVLQASQVSSYTDYGQE
DEIGLDISCNLGSLNILNVMKNKSLEQTVKLATDSLTHVSETTNITNAPAVKKANKAMKSIGLGAMNLHGFLAQNNIAYE
SEEARDFANTFFMMMNFYSIERSAEIAKEKGETYHGYEGSAYATGEYFEKYVTQDFSPKFEKVAKLFEGMHIPTKEDWKQ
LKQFVKEHGLYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLAPSNWFYYKEAYDMDMFRVVDM
ISTIQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQEGCLSCVV

Sequences:

>Translated_700_residues
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHDLKEKIDYLIKNDYYEEEFLSRYTFEEIKEV
FKEAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDKEKAKEFVRMMINQEYQPSTPTFLNAG
RKRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFIELAREDKAAYVFYPHTVYKAYGEHLDEMDMN
EMYDRLVEDPRVKKEKIHPRKMLEKLAMLRSESGYPYIMFQDNVNNVHANNHISKVKFSNLCSEVLQASQVSSYTDYGQE
DEIGLDISCNLGSLNILNVMKNKSLEQTVKLATDSLTHVSETTNITNAPAVKKANKAMKSIGLGAMNLHGFLAQNNIAYE
SEEARDFANTFFMMMNFYSIERSAEIAKEKGETYHGYEGSAYATGEYFEKYVTQDFSPKFEKVAKLFEGMHIPTKEDWKQ
LKQFVKEHGLYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLAPSNWFYYKEAYDMDMFRVVDM
ISTIQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQEGCLSCVV
>Mature_699_residues
SQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHDLKEKIDYLIKNDYYEEEFLSRYTFEEIKEVF
KEAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDKEKAKEFVRMMINQEYQPSTPTFLNAGR
KRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQMG
QRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFIELAREDKAAYVFYPHTVYKAYGEHLDEMDMNE
MYDRLVEDPRVKKEKIHPRKMLEKLAMLRSESGYPYIMFQDNVNNVHANNHISKVKFSNLCSEVLQASQVSSYTDYGQED
EIGLDISCNLGSLNILNVMKNKSLEQTVKLATDSLTHVSETTNITNAPAVKKANKAMKSIGLGAMNLHGFLAQNNIAYES
EEARDFANTFFMMMNFYSIERSAEIAKEKGETYHGYEGSAYATGEYFEKYVTQDFSPKFEKVAKLFEGMHIPTKEDWKQL
KQFVKEHGLYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLAPSNWFYYKEAYDMDMFRVVDMI
STIQQHVDQGISFTLFLKDTMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQEGCLSCVV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=629, Percent_Identity=25.1192368839428, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=43.6681222707424, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=665, Percent_Identity=22.7067669172932, Blast_Score=143, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17555242, Length=628, Percent_Identity=25, Blast_Score=186, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=24.4648318042813, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320914, Length=650, Percent_Identity=24.9230769230769, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17352481, Length=630, Percent_Identity=25.5555555555556, Blast_Score=189, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80817; Mature: 80686

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHDLKEKIDYLIK
CCCCCCCHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
NDYYEEEFLSRYTFEEIKEVFKEAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYED
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHH
RISIVALFFANGDKEKAKEFVRMMINQEYQPSTPTFLNAGRKRRGELVSCFLLEVNDSLN
HHEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
DISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
HHHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFIELAREDKAAYV
HCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCEEEE
FYPHTVYKAYGEHLDEMDMNEMYDRLVEDPRVKKEKIHPRKMLEKLAMLRSESGYPYIMF
EECHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEE
QDNVNNVHANNHISKVKFSNLCSEVLQASQVSSYTDYGQEDEIGLDISCNLGSLNILNVM
ECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHH
KNKSLEQTVKLATDSLTHVSETTNITNAPAVKKANKAMKSIGLGAMNLHGFLAQNNIAYE
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
SEEARDFANTFFMMMNFYSIERSAEIAKEKGETYHGYEGSAYATGEYFEKYVTQDFSPKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCCHHH
EKVAKLFEGMHIPTKEDWKQLKQFVKEHGLYHSYRLCIAPTGSISYVQSSTASVMPIMER
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCHHHHHHHHH
IEERTYGNSKTYYPMPGLAPSNWFYYKEAYDMDMFRVVDMISTIQQHVDQGISFTLFLKD
HHHHCCCCCCEECCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQEGCLSCVV
CCCCCCCCHHEEEHHHCCCCEEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
SQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHDLKEKIDYLIK
CCCCCCHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
NDYYEEEFLSRYTFEEIKEVFKEAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYED
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHH
RISIVALFFANGDKEKAKEFVRMMINQEYQPSTPTFLNAGRKRRGELVSCFLLEVNDSLN
HHEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
DISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
HHHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFIELAREDKAAYV
HCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCEEEE
FYPHTVYKAYGEHLDEMDMNEMYDRLVEDPRVKKEKIHPRKMLEKLAMLRSESGYPYIMF
EECHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEE
QDNVNNVHANNHISKVKFSNLCSEVLQASQVSSYTDYGQEDEIGLDISCNLGSLNILNVM
ECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHH
KNKSLEQTVKLATDSLTHVSETTNITNAPAVKKANKAMKSIGLGAMNLHGFLAQNNIAYE
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCC
SEEARDFANTFFMMMNFYSIERSAEIAKEKGETYHGYEGSAYATGEYFEKYVTQDFSPKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCCHHH
EKVAKLFEGMHIPTKEDWKQLKQFVKEHGLYHSYRLCIAPTGSISYVQSSTASVMPIMER
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCHHHHHHHHH
IEERTYGNSKTYYPMPGLAPSNWFYYKEAYDMDMFRVVDMISTIQQHVDQGISFTLFLKD
HHHHCCCCCCEECCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TMTTRDLNRIDLYAHHKGIKTLYYARTKDTGQEGCLSCVV
CCCCCCCCHHEEEHHHCCCCEEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]