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The map label for this gene is proS

Identifier: 52785637

GI number: 52785637

Start: 1830685

End: 1832403

Strand: Direct

Name: proS

Synonym: BLi01878

Alternate gene names: 52785637

Gene position: 1830685-1832403 (Clockwise)

Preceding gene: 52785636

Following gene: 52785638

Centisome position: 43.35

GC content: 49.8

Gene sequence:

>1719_bases
ATGAGACAGAGCAGGACTTTAATTCCTACGCTCCGCGAAGTGCCGGCTGATGCTGAAGCGAAAAGCCATCAGCTTCTTCT
CAGAGCAGGGTTTATCAGACAAAACACAAGCGGTGTATACAGCTATATGCCTCTTGCCAATAAAGTCATTCATAAAATCC
AGAGCATTGTCCGCGAAGAAATGGAGAAAATCAATGCCGTCGAAATGCTGATGCCGGCGCTCCAGCAGGCGGAAACTTGG
CAGGAATCGGGAAGATGGTATACGTACGGTCCTGAGCTCATGAGGCTGAAAGACCGCCACGGCCGTGAATTTGCCCTAGG
CGCAACACACGAAGAGGTCATCACAAGCATCGTCAGAGATGAAGTGAAATCGTACAAGCGCCTTCCGCTGACCCTTTACC
AGATCCAATCGAAGTTCCGCGATGAAAAGCGCCCGCGCTTCGGTCTGTTGAGAGGCCGGGAATTCATCATGAAGGATGCG
TATTCCTTCCATTCCTCCGCCGAAAGCCTTGATGAAACGTACAATGATATGTATCAGGCGTATACGAATGTGTTTACGCG
CTGCGGCCTGAATTTCAGACCGGTCATTGCAGACTCAGGCGCGATGGGCGGAAAGGATACCCATGAATTTATGGCATTGT
CCGATGTCGGTGAGGATACAATTGCGTATTCTGATCAGTCTTCATACGCCGCCAACATTGAAATGGCTGAAGTGAAGGAA
ACAGATGCCGGCGAGCAGGCAGAAATGAAAGAGCTGCAGGAAGTCCACACACCTTCGGTCAAAACGATCGAAGAGGTTGC
CGCATTTCTTGGCATATCCCCGTCAGACTGCATTAAATCAATGCTGATGAAAGCAGACGGACGTTTTGTCCTCGTCTTGA
CGAGAGGGGATCACGAAGTCAATGATGTTAAGGTGAAAAACCTGCTTCAAGCAGAAATAATCGAATTCGCGAGCGCCGAA
GAGGTTGCAGAGATAACAGGCACTGAACCCGGTTTTGTCGGACCGGTGGGGCTCGACCGCGAAATCGAAATCTTTGCAGA
CTTTGCTGTGAAGGCGATGGCAAACGCAGCAGCTGGAGCGAATAAAACAGATTACCATTATCAAAACGTGAATATCAGCC
GGGATGCGCACAATGTGACATTCGCCGATCTCCGCTTTATCCAGGAAGGAGACCCTTCACCGGATGGAAAAGGAACGATC
CGTTTTGCAAAAGGAATCGAAGTCGGACAAGTCTTTAAGCTCGGCACCCGCTATTCTGAAGCGATGGACGCCACATACCT
TGATGAAAACGGACGCGCTCAGCCGATGCTGATGGGCTGCTATGGAATCGGGATTTCCCGGACGCTTTCGGCCATCGTTG
AGCAGCATCATGACGATAAAGGCTTGATCTGGCCGCTGGAAGTGACGCCGTATGACCTGCATATCCTCGCGCTTAATATG
AAAAATGACGCGCAAGTTCAGCTTGCCGAAAAGCTGTATGAAGAATTTAAAGCGAACGGCTATGACGTACTGTTTGATGA
CCGTGCCGAACGGGCCGGCGTTAAATTTGCAGATTCCGATTTGATCGGTCTGCCGATCCGCATCACGGTCGGAAAAAGAG
CTGACGAAGGCGTTGTCGAAGTGAAGATCCGCAAAACCGGAGAATCCTTTGAAATCGCTGCTGATGAGCTGTTTGACTTT
ATCGAAAAACAAGTCAAGAGCTTATCTTCTCACTCTTAA

Upstream 100 bases:

>100_bases
CCTTTTTTAAGTGCAGCCAAGCCTTTTAAAAGGCTCTTTTTTAGGGACACCGTTCCCGACTCAAAAAAGTAACCAACTGA
AACAAATGAGGTGCGAATGA

Downstream 100 bases:

>100_bases
AACATGGTAAACTAGAGTAAAGGAAGGTACCTCGGGAATCGTCCCAAGGTACCTTCAATTATGATTTTCTTTTAGGGAGG
AATCTGTCTTGGAAGACCAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRQSRTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLANKVIHKIQSIVREEMEKINAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSIVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHSSAESLDETYNDMYQAYTNVFTRCGLNFRPVIADSGAMGGKDTHEFMALSDVGEDTIAYSDQSSYAANIEMAEVKE
TDAGEQAEMKELQEVHTPSVKTIEEVAAFLGISPSDCIKSMLMKADGRFVLVLTRGDHEVNDVKVKNLLQAEIIEFASAE
EVAEITGTEPGFVGPVGLDREIEIFADFAVKAMANAAAGANKTDYHYQNVNISRDAHNVTFADLRFIQEGDPSPDGKGTI
RFAKGIEVGQVFKLGTRYSEAMDATYLDENGRAQPMLMGCYGIGISRTLSAIVEQHHDDKGLIWPLEVTPYDLHILALNM
KNDAQVQLAEKLYEEFKANGYDVLFDDRAERAGVKFADSDLIGLPIRITVGKRADEGVVEVKIRKTGESFEIAADELFDF
IEKQVKSLSSHS

Sequences:

>Translated_572_residues
MRQSRTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLANKVIHKIQSIVREEMEKINAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSIVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHSSAESLDETYNDMYQAYTNVFTRCGLNFRPVIADSGAMGGKDTHEFMALSDVGEDTIAYSDQSSYAANIEMAEVKE
TDAGEQAEMKELQEVHTPSVKTIEEVAAFLGISPSDCIKSMLMKADGRFVLVLTRGDHEVNDVKVKNLLQAEIIEFASAE
EVAEITGTEPGFVGPVGLDREIEIFADFAVKAMANAAAGANKTDYHYQNVNISRDAHNVTFADLRFIQEGDPSPDGKGTI
RFAKGIEVGQVFKLGTRYSEAMDATYLDENGRAQPMLMGCYGIGISRTLSAIVEQHHDDKGLIWPLEVTPYDLHILALNM
KNDAQVQLAEKLYEEFKANGYDVLFDDRAERAGVKFADSDLIGLPIRITVGKRADEGVVEVKIRKTGESFEIAADELFDF
IEKQVKSLSSHS
>Mature_572_residues
MRQSRTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLANKVIHKIQSIVREEMEKINAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSIVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHSSAESLDETYNDMYQAYTNVFTRCGLNFRPVIADSGAMGGKDTHEFMALSDVGEDTIAYSDQSSYAANIEMAEVKE
TDAGEQAEMKELQEVHTPSVKTIEEVAAFLGISPSDCIKSMLMKADGRFVLVLTRGDHEVNDVKVKNLLQAEIIEFASAE
EVAEITGTEPGFVGPVGLDREIEIFADFAVKAMANAAAGANKTDYHYQNVNISRDAHNVTFADLRFIQEGDPSPDGKGTI
RFAKGIEVGQVFKLGTRYSEAMDATYLDENGRAQPMLMGCYGIGISRTLSAIVEQHHDDKGLIWPLEVTPYDLHILALNM
KNDAQVQLAEKLYEEFKANGYDVLFDDRAERAGVKFADSDLIGLPIRITVGKRADEGVVEVKIRKTGESFEIAADELFDF
IEKQVKSLSSHS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=262, Percent_Identity=41.6030534351145, Blast_Score=223, Evalue=5e-58,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=49.0401396160558, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI115532348, Length=248, Percent_Identity=33.8709677419355, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=32.5842696629214, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=536, Percent_Identity=27.9850746268657, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24656200, Length=268, Percent_Identity=33.2089552238806, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BACLD (Q65JJ1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079051.1
- RefSeq:   YP_091466.1
- ProteinModelPortal:   Q65JJ1
- SMR:   Q65JJ1
- STRING:   Q65JJ1
- EnsemblBacteria:   EBBACT00000058217
- EnsemblBacteria:   EBBACT00000061942
- GeneID:   3031073
- GeneID:   3101626
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01878
- KEGG:   bli:BL01235
- NMPDR:   fig|279010.5.peg.2582
- eggNOG:   COG0442
- GeneTree:   EBGT00050000001167
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   BLIC279010-1:BLI01878-MONOMER
- BioCyc:   BLIC279010:BL01235-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64138; Mature: 64138

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSRTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLANKVIHKIQSIVREE
CCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
MEKINAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRHGREFALGATHEEVITSIVRD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHSSAESLDETYNDMYQA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTNVFTRCGLNFRPVIADSGAMGGKDTHEFMALSDVGEDTIAYSDQSSYAANIEMAEVKE
HHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCEECCCHHHCCC
TDAGEQAEMKELQEVHTPSVKTIEEVAAFLGISPSDCIKSMLMKADGRFVLVLTRGDHEV
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
NDVKVKNLLQAEIIEFASAEEVAEITGTEPGFVGPVGLDREIEIFADFAVKAMANAAAGA
CHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
NKTDYHYQNVNISRDAHNVTFADLRFIQEGDPSPDGKGTIRFAKGIEVGQVFKLGTRYSE
CCCCCEEECCEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
AMDATYLDENGRAQPMLMGCYGIGISRTLSAIVEQHHDDKGLIWPLEVTPYDLHILALNM
HHCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEC
KNDAQVQLAEKLYEEFKANGYDVLFDDRAERAGVKFADSDLIGLPIRITVGKRADEGVVE
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCCEECCCCEEECEEEEEECCCCCCCEEE
VKIRKTGESFEIAADELFDFIEKQVKSLSSHS
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRQSRTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLANKVIHKIQSIVREE
CCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
MEKINAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRHGREFALGATHEEVITSIVRD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHSSAESLDETYNDMYQA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTNVFTRCGLNFRPVIADSGAMGGKDTHEFMALSDVGEDTIAYSDQSSYAANIEMAEVKE
HHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCEECCCHHHCCC
TDAGEQAEMKELQEVHTPSVKTIEEVAAFLGISPSDCIKSMLMKADGRFVLVLTRGDHEV
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
NDVKVKNLLQAEIIEFASAEEVAEITGTEPGFVGPVGLDREIEIFADFAVKAMANAAAGA
CHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
NKTDYHYQNVNISRDAHNVTFADLRFIQEGDPSPDGKGTIRFAKGIEVGQVFKLGTRYSE
CCCCCEEECCEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
AMDATYLDENGRAQPMLMGCYGIGISRTLSAIVEQHHDDKGLIWPLEVTPYDLHILALNM
HHCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEEC
KNDAQVQLAEKLYEEFKANGYDVLFDDRAERAGVKFADSDLIGLPIRITVGKRADEGVVE
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCCEECCCCEEECEEEEEECCCCCCCEEE
VKIRKTGESFEIAADELFDFIEKQVKSLSSHS
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA