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The map label for this gene is sigD [H]

Identifier: 52785627

GI number: 52785627

Start: 1821699

End: 1822463

Strand: Direct

Name: sigD [H]

Synonym: BLi01868

Alternate gene names: 52785627

Gene position: 1821699-1822463 (Clockwise)

Preceding gene: 52785626

Following gene: 52785628

Centisome position: 43.14

GC content: 46.67

Gene sequence:

>765_bases
ATGCAATCCTTGAACTATGAAGATCAGATGCTTTGGTCGCGGTGGAAAGAATGGAAAGATCCTCAGGCAGGCGATGATTT
AATCCGCCGCTATATGCCGCTTGTGACATATCATGTCGGCCGGATAGCCGTCGGCTTGCCCAAGTCCGTTCATAAAGACG
ATTTGGTCAGCCTTGGCATGCTGGGTTTGTATGATGCCCTTGAAAAATTTGATCCGGGAAGGGATTTGAAGTTTGATACA
TACGCTTCCTTCAGAATCAGAGGCGCTATCATCGACGGTCTTCGCAAAGAAGACTGGCTTCCGAGAACGTCCCGTGAAAA
AACGAAGAAAGTGGAAGCAGCGATCGAAAAGCTCGAACAGCGTTACTTAAGAAATGTTTCGCCGAGCGAAATCGCAGCTG
AGCTGGGAATGAGCGAACAAGACGTCGTGACCACAATCAATGAAGGTTTCTTTGCGAACCTCCTTTCAATTGATGAGAAG
CTTCACGACCATGAAGACGGGGAGAACGTCCAGGTGATGATAAGGGACGACAAAACGAGCACGCCTGAAGAAAAAATGCT
CAAAGACGAACTGATCTCCCAGCTTGCGGAAAAAATAAACGAGCTATCAGAAAAAGAGCAGCTCGTGATCAGCTTATTCT
ATAAAGAAGAGCTGACGCTGACGGAAATCGGACAGGTTTTGAATTTGTCGACTTCCCGTATTTCACAGATTCACTCCAAG
GCGCTTTTGAAATTGAAGCATCTGCTGGATAAAGTGGTGCAATAG

Upstream 100 bases:

>100_bases
GGAAGGACGATCGAGTTCGATCCGCAATCAGCTGAACTTGTCATCCGGACAGTTAAACAAGGTGTAACAACGATTTAATA
AGCAATATAGGGGGATAAAG

Downstream 100 bases:

>100_bases
CATGTAAGCTGCCGGCATGAAACCGGCAGCATTTTTTAAGCGTCCGACGGGAAGTCTTTTTTTGATTAAATTTCATGGTT
AGCGAGTGAAAAATATGTCA

Product: RNA polymerase sigma factor SigD

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQSLNYEDQMLWSRWKEWKDPQAGDDLIRRYMPLVTYHVGRIAVGLPKSVHKDDLVSLGMLGLYDALEKFDPGRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPSEIAAELGMSEQDVVTTINEGFFANLLSIDEK
LHDHEDGENVQVMIRDDKTSTPEEKMLKDELISQLAEKINELSEKEQLVISLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALLKLKHLLDKVVQ

Sequences:

>Translated_254_residues
MQSLNYEDQMLWSRWKEWKDPQAGDDLIRRYMPLVTYHVGRIAVGLPKSVHKDDLVSLGMLGLYDALEKFDPGRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPSEIAAELGMSEQDVVTTINEGFFANLLSIDEK
LHDHEDGENVQVMIRDDKTSTPEEKMLKDELISQLAEKINELSEKEQLVISLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALLKLKHLLDKVVQ
>Mature_254_residues
MQSLNYEDQMLWSRWKEWKDPQAGDDLIRRYMPLVTYHVGRIAVGLPKSVHKDDLVSLGMLGLYDALEKFDPGRDLKFDT
YASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQRYLRNVSPSEIAAELGMSEQDVVTTINEGFFANLLSIDEK
LHDHEDGENVQVMIRDDKTSTPEEKMLKDELISQLAEKINELSEKEQLVISLFYKEELTLTEIGQVLNLSTSRISQIHSK
ALLKLKHLLDKVVQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=226, Percent_Identity=39.3805309734513, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1789098, Length=213, Percent_Identity=27.6995305164319, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789448, Length=201, Percent_Identity=26.3681592039801, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29316; Mature: 29316

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLNYEDQMLWSRWKEWKDPQAGDDLIRRYMPLVTYHVGRIAVGLPKSVHKDDLVSLGM
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLYDALEKFDPGRDLKFDTYASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RYLRNVSPSEIAAELGMSEQDVVTTINEGFFANLLSIDEKLHDHEDGENVQVMIRDDKTS
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TPEEKMLKDELISQLAEKINELSEKEQLVISLFYKEELTLTEIGQVLNLSTSRISQIHSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
ALLKLKHLLDKVVQ
HHHHHHHHHHHHCC
>Mature Secondary Structure
MQSLNYEDQMLWSRWKEWKDPQAGDDLIRRYMPLVTYHVGRIAVGLPKSVHKDDLVSLGM
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLYDALEKFDPGRDLKFDTYASFRIRGAIIDGLRKEDWLPRTSREKTKKVEAAIEKLEQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RYLRNVSPSEIAAELGMSEQDVVTTINEGFFANLLSIDEKLHDHEDGENVQVMIRDDKTS
HHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
TPEEKMLKDELISQLAEKINELSEKEQLVISLFYKEELTLTEIGQVLNLSTSRISQIHSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHH
ALLKLKHLLDKVVQ
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]