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The map label for this gene is cheB

Identifier: 52785622

GI number: 52785622

Start: 1816933

End: 1818006

Strand: Direct

Name: cheB

Synonym: BLi01863

Alternate gene names: 52785622

Gene position: 1816933-1818006 (Clockwise)

Preceding gene: 52785621

Following gene: 52785623

Centisome position: 43.03

GC content: 51.02

Gene sequence:

>1074_bases
ATGATTCGTGTTCTTGTCGTTGACGACTCTGCTTTTATGAGAAACATGATCACCAAGTTTTTAACATCCAATCACGAGAT
TGCTGTAGCAGGAACGGCAAGAAACGGCGAGGAAGCGCTCCAAAAAATCAAGGAGCTCCGTCCGGATGTGATCACATTGG
ATATTGAAATGCCTGTGATGAACGGAAAAGAAACGTTGAAAAGGATTATGGCGAGCGATCCGCTTCCGGTTGTCATGGTA
TCGAGCCTGACGCAGCAGGGAGCAGATATCACGATCGAATGTCTGGAGCTTGGGGCGATCGATTTCGTCGCGAAGCCGTC
GGGTTCGATTTCAATAGATTTATATAAAGTAAGAGACATGCTGATTGAGAAGGTTTTGACAGCAGGACGGGTGAAACTGA
AAGGCCGGCAAGTTCCGATCTCAAAGCCCGCCATCGAGACGGCGAAGCAGCCGATTGTGGGCGGCAGCGATTCAGTCCGC
TTTCGGGCTGGAAAACAGCTCATCTGCATCGGTACATCGACAGGGGGGCCGAGGGCGCTGCAAAGAGTGCTTCCCAAGCT
TCCCAAAACGTTGAAGGCGCCTGTTTTTATCGTTCAGCACATGCCAAAGGGCTTTACGGCCTCATTAGCGAACAGGCTGA
ACCACCTCTCAGAAGTAACCGTGAAGGAGGCTGAAAACGGTGAGAGAGCGAAAGACGGCTGGGTTTACATTGCACCCGGA
GGAAAAAACATGGCGGTCGGCCTTGAAAAGGGCGAATTGGTGATCACGCTCGATGACCGCGACACAGAAAGCCGCCACAA
GCCTTCTGTTGACTACTTGTTTCAATCCCTGGCCTCACTTCGGGAGTTTGAAAAAATCGCCGTCATTATGACGGGGATGG
GAAGCGACGGAACAGAAGGCGTAAAAGGCCTTTTGAAACATGGGAGCGGAACGGTGATCGCCGAAGCAGCCGAGTCGTCC
GTCGTTTTCGGAATGCCGAAATCGGTCATTAACAACGGGCTTGCAAACGACATCAAGCATGTTGATGAGATTGCCGCAGC
CATTATGACGTATATGAAAAAAGAGAGGGCGTGA

Upstream 100 bases:

>100_bases
TGCTGGCCGAATCGCTGCTTCATAACGGCAGAACGGAAATGGGCGAAAACCGGTCCTTTATCGAAAAATTATCTTCATTC
CTGAGGAGGGGCAATACATT

Downstream 100 bases:

>100_bases
CTCATATGGATATGAATCAATATTTAGACGTCTTTATTGAAGAAAGCAGAGAACATTTGCAAACCTGTAACGAAAAATTG
CTCGAACTCGAAAAAAATCC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIRVLVVDDSAFMRNMITKFLTSNHEIAVAGTARNGEEALQKIKELRPDVITLDIEMPVMNGKETLKRIMASDPLPVVMV
SSLTQQGADITIECLELGAIDFVAKPSGSISIDLYKVRDMLIEKVLTAGRVKLKGRQVPISKPAIETAKQPIVGGSDSVR
FRAGKQLICIGTSTGGPRALQRVLPKLPKTLKAPVFIVQHMPKGFTASLANRLNHLSEVTVKEAENGERAKDGWVYIAPG
GKNMAVGLEKGELVITLDDRDTESRHKPSVDYLFQSLASLREFEKIAVIMTGMGSDGTEGVKGLLKHGSGTVIAEAAESS
VVFGMPKSVINNGLANDIKHVDEIAAAIMTYMKKERA

Sequences:

>Translated_357_residues
MIRVLVVDDSAFMRNMITKFLTSNHEIAVAGTARNGEEALQKIKELRPDVITLDIEMPVMNGKETLKRIMASDPLPVVMV
SSLTQQGADITIECLELGAIDFVAKPSGSISIDLYKVRDMLIEKVLTAGRVKLKGRQVPISKPAIETAKQPIVGGSDSVR
FRAGKQLICIGTSTGGPRALQRVLPKLPKTLKAPVFIVQHMPKGFTASLANRLNHLSEVTVKEAENGERAKDGWVYIAPG
GKNMAVGLEKGELVITLDDRDTESRHKPSVDYLFQSLASLREFEKIAVIMTGMGSDGTEGVKGLLKHGSGTVIAEAAESS
VVFGMPKSVINNGLANDIKHVDEIAAAIMTYMKKERA
>Mature_357_residues
MIRVLVVDDSAFMRNMITKFLTSNHEIAVAGTARNGEEALQKIKELRPDVITLDIEMPVMNGKETLKRIMASDPLPVVMV
SSLTQQGADITIECLELGAIDFVAKPSGSISIDLYKVRDMLIEKVLTAGRVKLKGRQVPISKPAIETAKQPIVGGSDSVR
FRAGKQLICIGTSTGGPRALQRVLPKLPKTLKAPVFIVQHMPKGFTASLANRLNHLSEVTVKEAENGERAKDGWVYIAPG
GKNMAVGLEKGELVITLDDRDTESRHKPSVDYLFQSLASLREFEKIAVIMTGMGSDGTEGVKGLLKHGSGTVIAEAAESS
VVFGMPKSVINNGLANDIKHVDEIAAAIMTYMKKERA

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=357, Percent_Identity=40.8963585434174, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=33.9622641509434, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_BACLD (Q65JK6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079036.1
- RefSeq:   YP_091451.1
- ProteinModelPortal:   Q65JK6
- SMR:   Q65JK6
- STRING:   Q65JK6
- EnsemblBacteria:   EBBACT00000055080
- EnsemblBacteria:   EBBACT00000060556
- GeneID:   3031051
- GeneID:   3101226
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01863
- KEGG:   bli:BL01251
- NMPDR:   fig|279010.5.peg.2566
- eggNOG:   COG2201
- GeneTree:   EBGT00050000000050
- HOGENOM:   HBG705324
- OMA:   FMRKMIS
- ProtClustDB:   PRK00742
- BioCyc:   BLIC279010-1:BLI01863-MONOMER
- BioCyc:   BLIC279010:BL01251-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38629; Mature: 38629

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY; PS00639 THIOL_PROTEASE_HIS

Important sites: ACT_SITE 173-173 ACT_SITE 200-200 ACT_SITE 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLVVDDSAFMRNMITKFLTSNHEIAVAGTARNGEEALQKIKELRPDVITLDIEMPVM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCC
NGKETLKRIMASDPLPVVMVSSLTQQGADITIECLELGAIDFVAKPSGSISIDLYKVRDM
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHH
LIEKVLTAGRVKLKGRQVPISKPAIETAKQPIVGGSDSVRFRAGKQLICIGTSTGGPRAL
HHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCCHHHH
QRVLPKLPKTLKAPVFIVQHMPKGFTASLANRLNHLSEVTVKEAENGERAKDGWVYIAPG
HHHHHHCCHHHCCHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
GKNMAVGLEKGELVITLDDRDTESRHKPSVDYLFQSLASLREFEKIAVIMTGMGSDGTEG
CCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHH
VKGLLKHGSGTVIAEAAESSVVFGMPKSVINNGLANDIKHVDEIAAAIMTYMKKERA
HHHHHHCCCCCEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRVLVVDDSAFMRNMITKFLTSNHEIAVAGTARNGEEALQKIKELRPDVITLDIEMPVM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEEECCC
NGKETLKRIMASDPLPVVMVSSLTQQGADITIECLELGAIDFVAKPSGSISIDLYKVRDM
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHH
LIEKVLTAGRVKLKGRQVPISKPAIETAKQPIVGGSDSVRFRAGKQLICIGTSTGGPRAL
HHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEEECCCCCHHHH
QRVLPKLPKTLKAPVFIVQHMPKGFTASLANRLNHLSEVTVKEAENGERAKDGWVYIAPG
HHHHHHCCHHHCCHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
GKNMAVGLEKGELVITLDDRDTESRHKPSVDYLFQSLASLREFEKIAVIMTGMGSDGTEG
CCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHH
VKGLLKHGSGTVIAEAAESSVVFGMPKSVINNGLANDIKHVDEIAAAIMTYMKKERA
HHHHHHCCCCCEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA