Click here to switch to the map view.

The map label for this gene is pyrK

Identifier: 52785532

GI number: 52785532

Start: 1732392

End: 1733162

Strand: Direct

Name: pyrK

Synonym: BLi01773

Alternate gene names: 52785532

Gene position: 1732392-1733162 (Clockwise)

Preceding gene: 52785531

Following gene: 52785533

Centisome position: 41.03

GC content: 52.01

Gene sequence:

>771_bases
ATGAGAAAAGGGTATATGACAGTCCGTTCAAACAAGTGCATCGCGGATCATATTTATCAAATGGTGCTTGAAGGGGAGAT
TGCCGAAGTTTGCGGCGCTCCCGGCCAGTTCCTTCACATCAAGGTCTCAGACTCCCTCACGCCGCTTTTGAGGCGGCCGA
TCAGCATCGCTGATATCAACAAAGCCGCCCGGCAGGTGACCATCATCTTTCGCAAAGATGGGGAAGGAACGAGACTCCTT
TCTTTAAAACAGGAAGGAGATCAGCTCGATGTTCTCGGTCCGCTCGGCAACGGTTTTCCGGCTGAATCGCTTGAACAGGG
GAAAACGGCTCTTCTCGTCGGAGGAGGGATCGGTGTTCCGCCGCTTTACGAGCTGAGCAAACAGCTGACAAACAGAGGGG
TGCACGCGATCCATGTGTTAGGATTTGCTTCTGCAAAAGATGTCTTTTACGAACAGGAATTCAGCCGTTTCGGCCGGGTG
TATGTGGCAACCGCAGACGGCACCCGCGGTTCAAAGGGATTTGTTACAGATGTCATTGAAAACGAGGGGCTTCAGTTTGA
CTGCATAATGGCCTGCGGACCGACGCCGATGCTGAAAGCTTTAAAACAGCGCTATCCGGATAAAGAGGTATATCTGTCAA
TGGAGGAGCGCATGGGATGCGGGATCGGCGCTTGCTTTGCCTGTGTCTGCCATACGGAAGAAAGCGAAACGTCTTATGTG
AAGGTATGCCTTGACGGTCCTGTTTTCAAAGCCCAGGAGGTGCTGTTATGA

Upstream 100 bases:

>100_bases
CCCTTGATACGGCGGAAGCGATTTTGCGGGTGCTTGAAAGCATGAGCTTCCGCGCCGATCATATGCCGGCGATTAAAATC
GCCCAAAAGGCGGCCGTCAG

Downstream 100 bases:

>100_bases
TGCTTGAAATCAATTTGCCGGGCCTTCAATTGAAAAATCCGATCATGCCGGCCTCAGGCTGCTTCGGATTCGGGAGAGAA
TTTGCGAACTTTTACGATCT

Product: dihydroorotate dehydrogenase electron transfer subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRKGYMTVRSNKCIADHIYQMVLEGEIAEVCGAPGQFLHIKVSDSLTPLLRRPISIADINKAARQVTIIFRKDGEGTRLL
SLKQEGDQLDVLGPLGNGFPAESLEQGKTALLVGGGIGVPPLYELSKQLTNRGVHAIHVLGFASAKDVFYEQEFSRFGRV
YVATADGTRGSKGFVTDVIENEGLQFDCIMACGPTPMLKALKQRYPDKEVYLSMEERMGCGIGACFACVCHTEESETSYV
KVCLDGPVFKAQEVLL

Sequences:

>Translated_256_residues
MRKGYMTVRSNKCIADHIYQMVLEGEIAEVCGAPGQFLHIKVSDSLTPLLRRPISIADINKAARQVTIIFRKDGEGTRLL
SLKQEGDQLDVLGPLGNGFPAESLEQGKTALLVGGGIGVPPLYELSKQLTNRGVHAIHVLGFASAKDVFYEQEFSRFGRV
YVATADGTRGSKGFVTDVIENEGLQFDCIMACGPTPMLKALKQRYPDKEVYLSMEERMGCGIGACFACVCHTEESETSYV
KVCLDGPVFKAQEVLL
>Mature_256_residues
MRKGYMTVRSNKCIADHIYQMVLEGEIAEVCGAPGQFLHIKVSDSLTPLLRRPISIADINKAARQVTIIFRKDGEGTRLL
SLKQEGDQLDVLGPLGNGFPAESLEQGKTALLVGGGIGVPPLYELSKQLTNRGVHAIHVLGFASAKDVFYEQEFSRFGRV
YVATADGTRGSKGFVTDVIENEGLQFDCIMACGPTPMLKALKQRYPDKEVYLSMEERMGCGIGACFACVCHTEESETSYV
KVCLDGPVFKAQEVLL

Specific function: Is responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the pyrD subunit to the ultimate electron acceptor NAD(+)

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRK_BACLD (Q65JU6)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078946.1
- RefSeq:   YP_091361.1
- HSSP:   P56968
- ProteinModelPortal:   Q65JU6
- SMR:   Q65JU6
- STRING:   Q65JU6
- EnsemblBacteria:   EBBACT00000055751
- EnsemblBacteria:   EBBACT00000058956
- GeneID:   3030925
- GeneID:   3098096
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01773
- KEGG:   bli:BL02277
- NMPDR:   fig|279010.5.peg.1715
- eggNOG:   COG0543
- GeneTree:   EBGT00050000000799
- HOGENOM:   HBG730970
- OMA:   RPISICS
- ProtClustDB:   PRK00054
- BioCyc:   BLIC279010-1:BLI01773-MONOMER
- BioCyc:   BLIC279010:BL02277-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01211_B
- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- PIRSF:   PIRSF006816

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: NA

Molecular weight: Translated: 27971; Mature: 27971

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGYMTVRSNKCIADHIYQMVLEGEIAEVCGAPGQFLHIKVSDSLTPLLRRPISIADIN
CCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCHHHHCCCCHHHHH
KAARQVTIIFRKDGEGTRLLSLKQEGDQLDVLGPLGNGFPAESLEQGKTALLVGGGIGVP
HCCEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCCCC
PLYELSKQLTNRGVHAIHVLGFASAKDVFYEQEFSRFGRVYVATADGTRGSKGFVTDVIE
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHH
NEGLQFDCIMACGPTPMLKALKQRYPDKEVYLSMEERMGCGIGACFACVCHTEESETSYV
CCCCEEEEEEECCCCHHHHHHHHHCCCHHEEEEEHHHCCCCHHHHHHEEECCCCCCCEEE
KVCLDGPVFKAQEVLL
EEEECCCCCCHHHHCC
>Mature Secondary Structure
MRKGYMTVRSNKCIADHIYQMVLEGEIAEVCGAPGQFLHIKVSDSLTPLLRRPISIADIN
CCCCCEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCHHHHCCCCHHHHH
KAARQVTIIFRKDGEGTRLLSLKQEGDQLDVLGPLGNGFPAESLEQGKTALLVGGGIGVP
HCCEEEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHCCCEEEEEECCCCCC
PLYELSKQLTNRGVHAIHVLGFASAKDVFYEQEFSRFGRVYVATADGTRGSKGFVTDVIE
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEHHHH
NEGLQFDCIMACGPTPMLKALKQRYPDKEVYLSMEERMGCGIGACFACVCHTEESETSYV
CCCCEEEEEEECCCCHHHHHHHHHCCCHHEEEEEHHHCCCCHHHHHHEEECCCCCCCEEE
KVCLDGPVFKAQEVLL
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA