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The map label for this gene is murD

Identifier: 52785496

GI number: 52785496

Start: 1692667

End: 1694022

Strand: Direct

Name: murD

Synonym: BLi01737

Alternate gene names: 52785496

Gene position: 1692667-1694022 (Clockwise)

Preceding gene: 52785495

Following gene: 52785497

Centisome position: 40.09

GC content: 49.56

Gene sequence:

>1356_bases
GTGGATACTCAAGATTTATTGCAAAATCAAAATGTGCTCGTACTGGGGCTGGCGAAAAGCGGATATGCTGCCGCTTCGAT
CCTTCATGAGAAAGGCGTAAAGGTTGTCGTGAATGACCAGCTGCCTTTTGAGGAAAATGAACCTGCGCGGATTCTCGCGG
AAAAAGGGGTGGAAGTCGTCTGCGGCTCTCATCCGACGGAACTGTTTGATCTTCATTCGATCGACATTCTGATTAAAAAT
CCGGGCATCCGCTATGAAAACGTGATGGTCGAAGAAGCGCTGAAGCGGGGTATCCCGGTCTGGACGGAGGTTGAACTCGC
CTATCATCTGACAGATGCTCCGTTCATCGGCATTACCGGTTCAAACGGAAAGACGACAACGACAACGCTTGTTTATGAAA
TGCTGAAGGCCGATTCGAAAAAAGCGCTCGTTGCTGGGAATATCGGTACGGCGGCAAGCGAAGTGGCCAATCAGGCCAGC
GGAGACGAATGGATCGTGACAGAGCTGTCGAGTTTTCAGCTCATGGGGACTTATCAATTCAGACCGAAGATTGGATTATT
ATTAAACGTATTTGATGCCCATCTCGATTATCACCATTCGCGCAAAAATTATGAGCTTGCCAAACAGCAGGTCTATCGAA
ATCAATCGGAAACAGACGTTGCCGTTGTCAATTTGGACGATGAGACAGTCGTCCGGCTCGCGGAGTGTTCAAAAGCGGAG
AAGGTGTATTTTTCGGTCCGCCGGACACTTGAGCGCGGAGCGTGCGTGAAAGACGGCGCAATCATGTTTAACGGCGAACC
GGTCATGCCGCTGGAAGACGTCGTTTTGCCGGGAGAGCACAATTTGGAGAACATTTTGGCCGCCCTTTGCATCGTTAAAA
CAGCAGGCGTTTCCGACGCGGCCGTCCGTAAGGTGCTGACGAGCTTTACAGGTGTTAAGCACAGGATGCAATACGTCGCG
ACGATCAAAAACAGACTGTTTTACAATGACAGCAAAGCGACAAACATTCTTGCGACGAAAAAAGCGCTGTCCGCCTTTCA
AAAGCCGGTCATTTTGCTGGCAGGGGGGCTTGACCGCGGAAATGAATTTGATGAACTAAAGCCGCATATGTCTTTTGTAA
AAGCGGTGATCACTTTCGGCGAGACCGCGCCGAAGTTTGAGAAGCTGGCCGAAGAAATGGGAATACAACAGGTTAAACGT
GTCGATAATGTTGAACAAGCAGCAACTGCGGCGTTCAGCCTGTCAGACGAAGGAGATGTCATTCTTCTGTCCCCGGCCTG
CGCAAGCTGGGATCAGTACAAAACATTTGAAGAACGTGGTGACATGTTTGTAAACGCCGTGCATATGCTTAAATAA

Upstream 100 bases:

>100_bases
CGAGCTTGTCGGATGGTCTGAGTGGAGAGTTGTCGTCACTTTTTGGACAGCGGGGCTTCTGCTTGCCGTCTTAGGAATTT
ACATCGAGGTGTGGTTATAA

Downstream 100 bases:

>100_bases
GGGCTTGTCTCGTAAAGATAGCCCTAAGAATTAGAGCTTGGGGTGTTCGGCTTTGCAAACAAAAAAAACGTCACCGGATT
TTTTGCTGGTTATCATTACG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MDTQDLLQNQNVLVLGLAKSGYAAASILHEKGVKVVVNDQLPFEENEPARILAEKGVEVVCGSHPTELFDLHSIDILIKN
PGIRYENVMVEEALKRGIPVWTEVELAYHLTDAPFIGITGSNGKTTTTTLVYEMLKADSKKALVAGNIGTAASEVANQAS
GDEWIVTELSSFQLMGTYQFRPKIGLLLNVFDAHLDYHHSRKNYELAKQQVYRNQSETDVAVVNLDDETVVRLAECSKAE
KVYFSVRRTLERGACVKDGAIMFNGEPVMPLEDVVLPGEHNLENILAALCIVKTAGVSDAAVRKVLTSFTGVKHRMQYVA
TIKNRLFYNDSKATNILATKKALSAFQKPVILLAGGLDRGNEFDELKPHMSFVKAVITFGETAPKFEKLAEEMGIQQVKR
VDNVEQAATAAFSLSDEGDVILLSPACASWDQYKTFEERGDMFVNAVHMLK

Sequences:

>Translated_451_residues
MDTQDLLQNQNVLVLGLAKSGYAAASILHEKGVKVVVNDQLPFEENEPARILAEKGVEVVCGSHPTELFDLHSIDILIKN
PGIRYENVMVEEALKRGIPVWTEVELAYHLTDAPFIGITGSNGKTTTTTLVYEMLKADSKKALVAGNIGTAASEVANQAS
GDEWIVTELSSFQLMGTYQFRPKIGLLLNVFDAHLDYHHSRKNYELAKQQVYRNQSETDVAVVNLDDETVVRLAECSKAE
KVYFSVRRTLERGACVKDGAIMFNGEPVMPLEDVVLPGEHNLENILAALCIVKTAGVSDAAVRKVLTSFTGVKHRMQYVA
TIKNRLFYNDSKATNILATKKALSAFQKPVILLAGGLDRGNEFDELKPHMSFVKAVITFGETAPKFEKLAEEMGIQQVKR
VDNVEQAATAAFSLSDEGDVILLSPACASWDQYKTFEERGDMFVNAVHMLK
>Mature_451_residues
MDTQDLLQNQNVLVLGLAKSGYAAASILHEKGVKVVVNDQLPFEENEPARILAEKGVEVVCGSHPTELFDLHSIDILIKN
PGIRYENVMVEEALKRGIPVWTEVELAYHLTDAPFIGITGSNGKTTTTTLVYEMLKADSKKALVAGNIGTAASEVANQAS
GDEWIVTELSSFQLMGTYQFRPKIGLLLNVFDAHLDYHHSRKNYELAKQQVYRNQSETDVAVVNLDDETVVRLAECSKAE
KVYFSVRRTLERGACVKDGAIMFNGEPVMPLEDVVLPGEHNLENILAALCIVKTAGVSDAAVRKVLTSFTGVKHRMQYVA
TIKNRLFYNDSKATNILATKKALSAFQKPVILLAGGLDRGNEFDELKPHMSFVKAVITFGETAPKFEKLAEEMGIQQVKR
VDNVEQAATAAFSLSDEGDVILLSPACASWDQYKTFEERGDMFVNAVHMLK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=455, Percent_Identity=32.967032967033, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BACLD (Q65JY2)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078913.1
- RefSeq:   YP_091325.1
- ProteinModelPortal:   Q65JY2
- SMR:   Q65JY2
- STRING:   Q65JY2
- EnsemblBacteria:   EBBACT00000058110
- EnsemblBacteria:   EBBACT00000061819
- GeneID:   3030887
- GeneID:   3098426
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01737
- KEGG:   bli:BL02241
- NMPDR:   fig|279010.5.peg.1584
- eggNOG:   COG0771
- GeneTree:   EBGT00070000032130
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   BLIC279010-1:BLI01737-MONOMER
- BioCyc:   BLIC279010:BL02241-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49740; Mature: 49740

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQDLLQNQNVLVLGLAKSGYAAASILHEKGVKVVVNDQLPFEENEPARILAEKGVEVV
CCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCEEE
CGSHPTELFDLHSIDILIKNPGIRYENVMVEEALKRGIPVWTEVELAYHLTDAPFIGITG
ECCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEC
SNGKTTTTTLVYEMLKADSKKALVAGNIGTAASEVANQASGDEWIVTELSSFQLMGTYQF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEEEE
RPKIGLLLNVFDAHLDYHHSRKNYELAKQQVYRNQSETDVAVVNLDDETVVRLAECSKAE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCHHH
KVYFSVRRTLERGACVKDGAIMFNGEPVMPLEDVVLPGEHNLENILAALCIVKTAGVSDA
HHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCHH
AVRKVLTSFTGVKHRMQYVATIKNRLFYNDSKATNILATKKALSAFQKPVILLAGGLDRG
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
NEFDELKPHMSFVKAVITFGETAPKFEKLAEEMGIQQVKRVDNVEQAATAAFSLSDEGDV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCCCE
ILLSPACASWDQYKTFEERGDMFVNAVHMLK
EEECCCCCCCHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MDTQDLLQNQNVLVLGLAKSGYAAASILHEKGVKVVVNDQLPFEENEPARILAEKGVEVV
CCCHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCEEE
CGSHPTELFDLHSIDILIKNPGIRYENVMVEEALKRGIPVWTEVELAYHLTDAPFIGITG
ECCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCEEEEEC
SNGKTTTTTLVYEMLKADSKKALVAGNIGTAASEVANQASGDEWIVTELSSFQLMGTYQF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEECCCEEEEEEEEE
RPKIGLLLNVFDAHLDYHHSRKNYELAKQQVYRNQSETDVAVVNLDDETVVRLAECSKAE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCHHH
KVYFSVRRTLERGACVKDGAIMFNGEPVMPLEDVVLPGEHNLENILAALCIVKTAGVSDA
HHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCHH
AVRKVLTSFTGVKHRMQYVATIKNRLFYNDSKATNILATKKALSAFQKPVILLAGGLDRG
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
NEFDELKPHMSFVKAVITFGETAPKFEKLAEEMGIQQVKRVDNVEQAATAAFSLSDEGDV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEECCCCCE
ILLSPACASWDQYKTFEERGDMFVNAVHMLK
EEECCCCCCCHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA