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The map label for this gene is murE

Identifier: 52785494

GI number: 52785494

Start: 1690098

End: 1691564

Strand: Direct

Name: murE

Synonym: BLi01735

Alternate gene names: 52785494

Gene position: 1690098-1691564 (Clockwise)

Preceding gene: 52785493

Following gene: 52785495

Centisome position: 40.02

GC content: 50.24

Gene sequence:

>1467_bases
ATGAAATTGACAAAGCTTCTTACATATTTAAAAAATGTACCTTCCTATGCCGGCCAAGAAGACCCTGATATAACTTCAAT
TGAGATGGACTCCAGGGAAGTCAAGACAGGGAGTCTTTTTGTTTGTATAAAAGGCTATACAGTCGACGGGCATGACTACG
CCCGCCAAGCCGCTGAAAAAGGGGCCGCCGCCATTGTGGCGGAAAGGGAAGTGGACGCCGATGTGCCGGTGATTATCATC
CGTCATACGAAGAGAGCGCTTGCCGTGCTGTCGGACGCTTTTTACGGCCAGCCGACAAAGCAGCTGAGGCTGATCGGAAT
TACAGGCACGAACGGCAAGACATCGACTTCTCACATGGTGGAGGAGATCTTTAGAAAAGCCGGGAGCCGTACAGGACTGA
TCGGCACGATGTACACGAAAATCCACGACGAAACGTTCGAGGCGAAAAATACGACGCCGGAAAGCGTCACACTTCAAAAA
ACGTTTAGAAAAATGGTTGATCAGGGTGTGGATACAGCCGTCATGGAAGTATCTTCACACGCTCTCCACATGGGCCGGGT
GCACGGGTGTGATTACGATATCGCCGCATTTACAAACCTGACCCAGGACCACCTCGACTACCACGAGACAATGGAAGAAT
ATAAACATGCGAAAAGTCTGCTTTTCTCGCAGCTCGGCGGATCCTTTAACCACGAAACACCGAAGTGGGCCGTGCTGAAT
GCGGATGACCCTGCTTCAGCCTATTTCGCCCAAGTGACGTCAGCACATCTTTTGACATACGGAATTCAAAACGATGCCGA
TGTCATGGCTGAAAATATCAAAATGGCTCCGAAAGGGACAACCTTTGACCTGGTGACGCCAAAAGGTTCTGCACAAGTGA
CGATTCCGCTTGTCGGCCTGTTTAACGTCTACAATGTTTTAACGGCTGCCGCGATCGCGATTGCAGCGGATATCCCGTTT
GCCACGATTACAGAAGGAATTGAAGGGCTGAAAGGGGTCAGAGGAAGATTTGAGCTTGTGGACGCAGGCCAGGACTTCCC
GGTGATCGTCGATTATGCCCATACCCCTGACAGCCTCGAAAATGTCTTGAATACGTGCAGAGGCTTAACGGAAGGAAAGC
TGTTTGTCGTCGTCGGCTGCGGTGGAGACCGCGATAAGACGAAGCGTCCGAAAATGGCGAAGATCGCCGTTGACCTTGCT
GATGAACCCGTCTTTACAGCCGATAACCCGAGAAGCGAAAACCCGCTCGCCATTTTAAACGATATGGAGGAAGGCGTAAA
AGGAGCTTACTATCACAGCATCGTCAACCGCGAGCAAGCGATCTTTTTTGCAATTGCAAACGCCAAAAAAGGCGATGTCG
TCCTGATTGCAGGCAAAGGCCATGAAACTTACCAGCAGATCGGCGGTCAAACCTTTGATTTTGATGACGCTGAGGTGGCC
AAAAGAGCCATTCTTGAGCTGAAATAA

Upstream 100 bases:

>100_bases
TTTTTACGCTAAAATATGAACTGTATGTCTGCACGAAGCTCGTGCGGCGAATCAATATGACAACGAATGAAGAATGATGA
GACTTGAGAGGTTTGATACG

Downstream 100 bases:

>100_bases
TCGAAAAAGAACTACAGGAAAGAGTTGTTGAAATGATGGATGAAATAAATCAACATATGAAAAAAAACAGTGAATATGAT
AAATGCGTATGCTGCTTCTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKLTKLLTYLKNVPSYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAEKGAAAIVAEREVDADVPVIII
RHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKTSTSHMVEEIFRKAGSRTGLIGTMYTKIHDETFEAKNTTPESVTLQK
TFRKMVDQGVDTAVMEVSSHALHMGRVHGCDYDIAAFTNLTQDHLDYHETMEEYKHAKSLLFSQLGGSFNHETPKWAVLN
ADDPASAYFAQVTSAHLLTYGIQNDADVMAENIKMAPKGTTFDLVTPKGSAQVTIPLVGLFNVYNVLTAAAIAIAADIPF
ATITEGIEGLKGVRGRFELVDAGQDFPVIVDYAHTPDSLENVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLA
DEPVFTADNPRSENPLAILNDMEEGVKGAYYHSIVNREQAIFFAIANAKKGDVVLIAGKGHETYQQIGGQTFDFDDAEVA
KRAILELK

Sequences:

>Translated_488_residues
MKLTKLLTYLKNVPSYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAEKGAAAIVAEREVDADVPVIII
RHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKTSTSHMVEEIFRKAGSRTGLIGTMYTKIHDETFEAKNTTPESVTLQK
TFRKMVDQGVDTAVMEVSSHALHMGRVHGCDYDIAAFTNLTQDHLDYHETMEEYKHAKSLLFSQLGGSFNHETPKWAVLN
ADDPASAYFAQVTSAHLLTYGIQNDADVMAENIKMAPKGTTFDLVTPKGSAQVTIPLVGLFNVYNVLTAAAIAIAADIPF
ATITEGIEGLKGVRGRFELVDAGQDFPVIVDYAHTPDSLENVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLA
DEPVFTADNPRSENPLAILNDMEEGVKGAYYHSIVNREQAIFFAIANAKKGDVVLIAGKGHETYQQIGGQTFDFDDAEVA
KRAILELK
>Mature_488_residues
MKLTKLLTYLKNVPSYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAEKGAAAIVAEREVDADVPVIII
RHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKTSTSHMVEEIFRKAGSRTGLIGTMYTKIHDETFEAKNTTPESVTLQK
TFRKMVDQGVDTAVMEVSSHALHMGRVHGCDYDIAAFTNLTQDHLDYHETMEEYKHAKSLLFSQLGGSFNHETPKWAVLN
ADDPASAYFAQVTSAHLLTYGIQNDADVMAENIKMAPKGTTFDLVTPKGSAQVTIPLVGLFNVYNVLTAAAIAIAADIPF
ATITEGIEGLKGVRGRFELVDAGQDFPVIVDYAHTPDSLENVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLA
DEPVFTADNPRSENPLAILNDMEEGVKGAYYHSIVNREQAIFFAIANAKKGDVVLIAGKGHETYQQIGGQTFDFDDAEVA
KRAILELK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=37.5770020533881, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1786274, Length=332, Percent_Identity=29.5180722891566, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_BACLD (Q65JY4)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078911.1
- RefSeq:   YP_091323.1
- HSSP:   P22188
- ProteinModelPortal:   Q65JY4
- SMR:   Q65JY4
- STRING:   Q65JY4
- EnsemblBacteria:   EBBACT00000054733
- EnsemblBacteria:   EBBACT00000061083
- GeneID:   3030883
- GeneID:   3099138
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01735
- KEGG:   bli:BL02239
- NMPDR:   fig|279010.5.peg.1582
- eggNOG:   COG0769
- GeneTree:   EBGT00050000000629
- HOGENOM:   HBG602753
- OMA:   IGTIANY
- ProtClustDB:   PRK00139
- BioCyc:   BLIC279010-1:BLI01735-MONOMER
- BioCyc:   BLIC279010:BL02239-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53243; Mature: 53243

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: BINDING 31-31 BINDING 150-150 BINDING 178-178 BINDING 186-186 BINDING 384-384 BINDING 458-458 BINDING 462-462

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKLLTYLKNVPSYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAEK
CCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEECCCEEEEEEEEEEECCHHHHHHHHHC
GAAAIVAEREVDADVPVIIIRHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKTSTSHMV
CCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHH
EEIFRKAGSRTGLIGTMYTKIHDETFEAKNTTPESVTLQKTFRKMVDQGVDTAVMEVSSH
HHHHHHCCCCCCEEEEHHHHHCCHHHCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHH
ALHMGRVHGCDYDIAAFTNLTQDHLDYHETMEEYKHAKSLLFSQLGGSFNHETPKWAVLN
HHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADDPASAYFAQVTSAHLLTYGIQNDADVMAENIKMAPKGTTFDLVTPKGSAQVTIPLVGL
CCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHCEECCCCCEEEEECCCCCCEEEEEHHHH
FNVYNVLTAAAIAIAADIPFATITEGIEGLKGVRGRFELVDAGQDFPVIVDYAHTPDSLE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
NVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLADEPVFTADNPRSENPLAILN
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCEEEHH
DMEEGVKGAYYHSIVNREQAIFFAIANAKKGDVVLIAGKGHETYQQIGGQTFDFDDAEVA
HHHHHHCHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHH
KRAILELK
HHHHHCCC
>Mature Secondary Structure
MKLTKLLTYLKNVPSYAGQEDPDITSIEMDSREVKTGSLFVCIKGYTVDGHDYARQAAEK
CCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEECCCEEEEEEEEEEECCHHHHHHHHHC
GAAAIVAEREVDADVPVIIIRHTKRALAVLSDAFYGQPTKQLRLIGITGTNGKTSTSHMV
CCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCCHHHHHH
EEIFRKAGSRTGLIGTMYTKIHDETFEAKNTTPESVTLQKTFRKMVDQGVDTAVMEVSSH
HHHHHHCCCCCCEEEEHHHHHCCHHHCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHH
ALHMGRVHGCDYDIAAFTNLTQDHLDYHETMEEYKHAKSLLFSQLGGSFNHETPKWAVLN
HHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADDPASAYFAQVTSAHLLTYGIQNDADVMAENIKMAPKGTTFDLVTPKGSAQVTIPLVGL
CCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHCEECCCCCEEEEECCCCCCEEEEEHHHH
FNVYNVLTAAAIAIAADIPFATITEGIEGLKGVRGRFELVDAGQDFPVIVDYAHTPDSLE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHH
NVLNTCRGLTEGKLFVVVGCGGDRDKTKRPKMAKIAVDLADEPVFTADNPRSENPLAILN
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCEEEHH
DMEEGVKGAYYHSIVNREQAIFFAIANAKKGDVVLIAGKGHETYQQIGGQTFDFDDAEVA
HHHHHHCHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHH
KRAILELK
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA