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The map label for this gene is ykwC [H]

Identifier: 52785369

GI number: 52785369

Start: 1571337

End: 1572209

Strand: Direct

Name: ykwC [H]

Synonym: BLi01606

Alternate gene names: 52785369

Gene position: 1571337-1572209 (Clockwise)

Preceding gene: 52785368

Following gene: 52785371

Centisome position: 37.21

GC content: 51.43

Gene sequence:

>873_bases
ATGAAAAAAACGGTCGGATTTATCGGACTCGGTGTAATGGGAAACAGCATGGCCTCGCACATTTTAGCCGCGGGCTATCC
CGTCAGCGCCTATACGAGAACGAAACACAAAGCGGACAGCCTCGTGGAAAAAGGGGCGGAATGGAAATCATCCGTCAAAG
CACTGGCGCAGTCGTCTGATGTGATCATCACAATGGTCGGCTACCCAAAAGACGTTGAAGAGGTTTACTTTGGAAGCGAA
GGCATTATTGAAAATGCCAAAAAAGGTTCCTACCTTATCGATATGACGACTTCCAAACCTTCGCTTGCCAAACAAATCGA
GACTGCCGCAAAAGAGAAAGGACTTTACGCTTTGGATGCTCCGGTTTCAGGCGGGGACGTCGGCGCGAGGAACGGCACGC
TCGCTATCATGGTCGGAGGAGAACGGAAAGCTTATGATGAATGCTACCCGCTCTTTTCGATCATGGGTGAAAACATCCAG
TATCAGGGGCCGGCCGGAAGCGGCCAGCATACGAAAATGTGCAACCAGATTGCGATTGCCGCAGGGATGATCGGCGTCGC
AGAAGCGATGGCCTACGCCGAAAAATCCGGACTCGATCCCGACAACGTGCTGAAAAGCATTACGACCGGCGCTGCGGGAA
GCTGGTCGCTCTCAAATCTAGCGCCTAGAATGCTGAAAGGCGACTTTGAACCGGGTTTTTACGTCAAACACTTTGTTAAA
GACATGGGCATCGCGCTTGAAGAGGCGGAGCTGATGGGCGAGAAAATGCCGGGGCTCGAGCTTGCGAAAAGCCTTTACGA
CACCCTTGTTGAAAAAGGCGAAGAAAACAGCGGCACCCAAAGTCTGTACAAGCTTTGGACAGAATACAAATAA

Upstream 100 bases:

>100_bases
TATGCAAGCTTTCTTTTATCAGAGTGCAATACATATATGACTGGACAAACGCTGCTGATCGACGGCGGAATGGTAAAAGC
AATTTAAGGGGGATGAGACC

Downstream 100 bases:

>100_bases
CGAAAAAAACCACTTGCTGCATGCAAGTGGTTTTTTGCTGGTTCTTATTTTCCGATGAACTGCTGAGTCCAGTAGTTGCC
GTCCTTCACATATCCGACAC

Product: YkwC

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKKTVGFIGLGVMGNSMASHILAAGYPVSAYTRTKHKADSLVEKGAEWKSSVKALAQSSDVIITMVGYPKDVEEVYFGSE
GIIENAKKGSYLIDMTTSKPSLAKQIETAAKEKGLYALDAPVSGGDVGARNGTLAIMVGGERKAYDECYPLFSIMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAEKSGLDPDNVLKSITTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFVK
DMGIALEEAELMGEKMPGLELAKSLYDTLVEKGEENSGTQSLYKLWTEYK

Sequences:

>Translated_290_residues
MKKTVGFIGLGVMGNSMASHILAAGYPVSAYTRTKHKADSLVEKGAEWKSSVKALAQSSDVIITMVGYPKDVEEVYFGSE
GIIENAKKGSYLIDMTTSKPSLAKQIETAAKEKGLYALDAPVSGGDVGARNGTLAIMVGGERKAYDECYPLFSIMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAEKSGLDPDNVLKSITTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFVK
DMGIALEEAELMGEKMPGLELAKSLYDTLVEKGEENSGTQSLYKLWTEYK
>Mature_290_residues
MKKTVGFIGLGVMGNSMASHILAAGYPVSAYTRTKHKADSLVEKGAEWKSSVKALAQSSDVIITMVGYPKDVEEVYFGSE
GIIENAKKGSYLIDMTTSKPSLAKQIETAAKEKGLYALDAPVSGGDVGARNGTLAIMVGGERKAYDECYPLFSIMGENIQ
YQGPAGSGQHTKMCNQIAIAAGMIGVAEAMAYAEKSGLDPDNVLKSITTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFVK
DMGIALEEAELMGEKMPGLELAKSLYDTLVEKGEENSGTQSLYKLWTEYK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=296, Percent_Identity=35.1351351351351, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI40556376, Length=284, Percent_Identity=24.6478873239437, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI145693186, Length=253, Percent_Identity=40.7114624505929, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786719, Length=269, Percent_Identity=37.546468401487, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1790315, Length=255, Percent_Identity=30.9803921568627, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=29.020979020979, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=29.0441176470588, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=33.5593220338983, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=33.5593220338983, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI28574115, Length=267, Percent_Identity=26.5917602996255, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31028; Mature: 31028

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVGFIGLGVMGNSMASHILAAGYPVSAYTRTKHKADSLVEKGAEWKSSVKALAQSSD
CCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VIITMVGYPKDVEEVYFGSEGIIENAKKGSYLIDMTTSKPSLAKQIETAAKEKGLYALDA
EEEEEECCCCCHHHHHCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEC
PVSGGDVGARNGTLAIMVGGERKAYDECYPLFSIMGENIQYQGPAGSGQHTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
AGMIGVAEAMAYAEKSGLDPDNVLKSITTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFVK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
DMGIALEEAELMGEKMPGLELAKSLYDTLVEKGEENSGTQSLYKLWTEYK
HHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKTVGFIGLGVMGNSMASHILAAGYPVSAYTRTKHKADSLVEKGAEWKSSVKALAQSSD
CCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
VIITMVGYPKDVEEVYFGSEGIIENAKKGSYLIDMTTSKPSLAKQIETAAKEKGLYALDA
EEEEEECCCCCHHHHHCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEC
PVSGGDVGARNGTLAIMVGGERKAYDECYPLFSIMGENIQYQGPAGSGQHTKMCNQIAIA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
AGMIGVAEAMAYAEKSGLDPDNVLKSITTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFVK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
DMGIALEEAELMGEKMPGLELAKSLYDTLVEKGEENSGTQSLYKLWTEYK
HHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]