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The map label for this gene is queF

Identifier: 52785339

GI number: 52785339

Start: 1542605

End: 1542802

Strand: Direct

Name: queF

Synonym: BLi01575

Alternate gene names: 52785339

Gene position: 1542605-1542802 (Clockwise)

Preceding gene: 52785338

Following gene: 52785349

Centisome position: 36.53

GC content: 49.49

Gene sequence:

>198_bases
ATGAACATCATCATGAACGATTTAATTGAGCTGATGGACCCGCGCTACATCGAAGTCTGGGGCAAATTCACGCCGAGGGG
CGGAATCTCGATCGACCCGTACACCAACTACGGCAAGCCCGGCACAAAGTATGAAAAAATGGCCGAGTATCGGATGATGA
ACCATGATCTGTATCCGGAGACGATTGATAATCGGTGA

Upstream 100 bases:

>100_bases
AGTCGCGAAAGTGTGACCGCAGTGTGACCGTTTTGTGTGACCGGATGTGAGAGCGTTGATATGACGGGATTTTAACAACG
GATTCCATCACGAAGACTGC

Downstream 100 bases:

>100_bases
TGATGCGGGCAGATGAAGCGAAGGTTTGTGATCGATGTGTGACCGAGACGCGTACTGTTACGCGTCTTTTTTGTTCGAAA
TTTCCACGAACAATTACCAA

Product: hypothetical protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 65; Mature: 65

Protein sequence:

>65_residues
MNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPETIDNR

Sequences:

>Translated_65_residues
MNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPETIDNR
>Mature_65_residues
MNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPETIDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_BACLD (Q65KI3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078715.1
- RefSeq:   YP_091124.1
- ProteinModelPortal:   Q65KI3
- SMR:   Q65KI3
- STRING:   Q65KI3
- EnsemblBacteria:   EBBACT00000056754
- EnsemblBacteria:   EBBACT00000061561
- GeneID:   3030614
- GeneID:   3099344
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01530
- KEGG:   bli:BL03549
- NMPDR:   fig|279010.5.peg.1082
- eggNOG:   COG0780
- GeneTree:   EBGT00050000002144
- HOGENOM:   HBG294103
- OMA:   CINTILL
- ProtClustDB:   PRK13258
- BioCyc:   BLIC279010-1:BLI01530-MONOMER
- BioCyc:   BLIC279010:BL03549-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 7748; Mature: 7748

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
9.2 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
9.2 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPE
CCCHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TIDNR
CCCCC
>Mature Secondary Structure
MNIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPE
CCCHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TIDNR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA