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The map label for this gene is appA [H]

Identifier: 52784998

GI number: 52784998

Start: 1236128

End: 1237771

Strand: Direct

Name: appA [H]

Synonym: BLi01226

Alternate gene names: 52784998

Gene position: 1236128-1237771 (Clockwise)

Preceding gene: 52784997

Following gene: 52784999

Centisome position: 29.27

GC content: 41.0

Gene sequence:

>1644_bases
ATGAATAAACGCAAAACAGGATTTTCAATTTTAAGCTTGCTGCTGATCTTATCGATTTTTCTAACGGCCTGCAACAGCGG
CGAAGTCGGAGGGGATGAAAAAGAAGGCAAATCTGACGGGAAGCCGCAGCAGGGCGGAGATCTGATTGCTGGATCCACAG
GTGAACCGACGCTGTTTAATTCACTGTATTCAACTGATACAGCAAGTTCAGATATTGAAAGACTGATCTACAACACTTTG
TTAGACGTTAATGAGAAACTTGAAGTGGAAAACCAACTCGCTGATGAAGTAAAGGAATCGGAGGATGGGTTAACATTCGA
TGTGAAGCTAAAAGAAGGTGTCAAGTTTCACGACGGTGAAGAAATGACAGCCGACGATGTCGTTTTTACTTACAGCATTC
CGATGAGTGATGAATACGTTGGAGAACGCGGCTCAAACTTTAAAATGATAGAGTCTGTCACGAAAAAAGGTAAATATGAA
GTGCAGTTTAAATTAAAGAAGCAGGATCCGTATTTTTACAATGTTACACTTGCCAGTTACGGTATTCTGCCTAAGCACAT
TTTAAAAGATGTCCCAATCAGCAAACTCGGTGAACACGAATTCAATCGAAAGAATCCGATTGGAACAGGACCGTTTAAAT
TTAAAGAATGGAAAGAAGGACAATATGTAAAGGTTGAGGCTTTTGATGATTATTATGCCGGGCGTCCTCATTTAGATTCG
ATCACGTATAAAATTATTCCGGATTCAAATGCAGCGCTGTCACAGCTGCAAGCTGGAGATGTCGATTACTTGGTCGTTAC
ACCAGGACCAGACTATAAAACGGCCGAGAAATTTAACAATGTGAAGATGGAAACCGATTTAGGTTTGAATTATACGTATA
TCGGCTGGAACGAAAGAAATGAGCTGTTTAAGGATAAAAAGGTTCGCCAAGCGCTGACACATGCGCTTGACCGCCAGGCG
CTCGTTGACCAAGTTCTAGATGGAGATGGGGAAATCGCGAACATCCCGGAAAGCCCGCTTTCATGGAACTACCCGGATAA
CAAAGATAAGTTTAAAACGTTTGAATACGATCCAGAGAAAGCAAAAAAACTGCTCAAAGAAGCAGGATGGACAGACTCAG
ATGGTGATGGGATTTTAGATAAGGACGGCAAAAAGTTTTCTTTCGTTATTAAAACGAACCAAGGAAACAAAACACGGGAA
GACCTCGCGGTTGTTGTTCAGCAGCAATTAAAAGAAATTGGGATTCAAGCAAAACCGCAGATTGTTGAATGGAGTGCTTT
AATTGAACAAATGAACCCGCCGAATTGGGATTTTGATGCGATGATCATGGGCTGGAGCCTTGCTACGTTCCCTGATCAGA
GCAACATTTTCCATTCGAAAGAAGCTGAAAAAGGACTAAACTATGTTTGGTATCAAAATGAAAAGCTTGATAAACTGCTG
GATGAAGCTAAAACGTTGAAGGACCGTGAAGAATATAAAAAGGCGTATGAAGATATTTACGAAATTCTGGCGGAAGATCA
GCCGTATACATTCTTGTATTATACGAATTACCATAGAGCGATGCCTAAAAATATGAAAGGCTACGTATTCCATCCGAAAG
AAGATTTCTATAAAGCCGAAGACTGGTGGCTGGATCAAAAATAA

Upstream 100 bases:

>100_bases
TCACATATTAAATCCTTGAGAGGGGGAATGTCTTCTTTAAAAAACGGGGGTCAATCGGGTGGAAAGAAGGACAACAGTTT
AATAAGGGGGAATTCATAAA

Downstream 100 bases:

>100_bases
CGAGAATCCCTTGAGGGAAGTGTAGCTTGCAGCTTCCTTCCCTTCCTTCTTAATGACTTTTAAAAAAGGGGATAAAAAAT
GATTACATATATCATTCGAA

Product: AppA

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNKRKTGFSILSLLLILSIFLTACNSGEVGGDEKEGKSDGKPQQGGDLIAGSTGEPTLFNSLYSTDTASSDIERLIYNTL
LDVNEKLEVENQLADEVKESEDGLTFDVKLKEGVKFHDGEEMTADDVVFTYSIPMSDEYVGERGSNFKMIESVTKKGKYE
VQFKLKKQDPYFYNVTLASYGILPKHILKDVPISKLGEHEFNRKNPIGTGPFKFKEWKEGQYVKVEAFDDYYAGRPHLDS
ITYKIIPDSNAALSQLQAGDVDYLVVTPGPDYKTAEKFNNVKMETDLGLNYTYIGWNERNELFKDKKVRQALTHALDRQA
LVDQVLDGDGEIANIPESPLSWNYPDNKDKFKTFEYDPEKAKKLLKEAGWTDSDGDGILDKDGKKFSFVIKTNQGNKTRE
DLAVVVQQQLKEIGIQAKPQIVEWSALIEQMNPPNWDFDAMIMGWSLATFPDQSNIFHSKEAEKGLNYVWYQNEKLDKLL
DEAKTLKDREEYKKAYEDIYEILAEDQPYTFLYYTNYHRAMPKNMKGYVFHPKEDFYKAEDWWLDQK

Sequences:

>Translated_547_residues
MNKRKTGFSILSLLLILSIFLTACNSGEVGGDEKEGKSDGKPQQGGDLIAGSTGEPTLFNSLYSTDTASSDIERLIYNTL
LDVNEKLEVENQLADEVKESEDGLTFDVKLKEGVKFHDGEEMTADDVVFTYSIPMSDEYVGERGSNFKMIESVTKKGKYE
VQFKLKKQDPYFYNVTLASYGILPKHILKDVPISKLGEHEFNRKNPIGTGPFKFKEWKEGQYVKVEAFDDYYAGRPHLDS
ITYKIIPDSNAALSQLQAGDVDYLVVTPGPDYKTAEKFNNVKMETDLGLNYTYIGWNERNELFKDKKVRQALTHALDRQA
LVDQVLDGDGEIANIPESPLSWNYPDNKDKFKTFEYDPEKAKKLLKEAGWTDSDGDGILDKDGKKFSFVIKTNQGNKTRE
DLAVVVQQQLKEIGIQAKPQIVEWSALIEQMNPPNWDFDAMIMGWSLATFPDQSNIFHSKEAEKGLNYVWYQNEKLDKLL
DEAKTLKDREEYKKAYEDIYEILAEDQPYTFLYYTNYHRAMPKNMKGYVFHPKEDFYKAEDWWLDQK
>Mature_547_residues
MNKRKTGFSILSLLLILSIFLTACNSGEVGGDEKEGKSDGKPQQGGDLIAGSTGEPTLFNSLYSTDTASSDIERLIYNTL
LDVNEKLEVENQLADEVKESEDGLTFDVKLKEGVKFHDGEEMTADDVVFTYSIPMSDEYVGERGSNFKMIESVTKKGKYE
VQFKLKKQDPYFYNVTLASYGILPKHILKDVPISKLGEHEFNRKNPIGTGPFKFKEWKEGQYVKVEAFDDYYAGRPHLDS
ITYKIIPDSNAALSQLQAGDVDYLVVTPGPDYKTAEKFNNVKMETDLGLNYTYIGWNERNELFKDKKVRQALTHALDRQA
LVDQVLDGDGEIANIPESPLSWNYPDNKDKFKTFEYDPEKAKKLLKEAGWTDSDGDGILDKDGKKFSFVIKTNQGNKTRE
DLAVVVQQQLKEIGIQAKPQIVEWSALIEQMNPPNWDFDAMIMGWSLATFPDQSNIFHSKEAEKGLNYVWYQNEKLDKLL
DEAKTLKDREEYKKAYEDIYEILAEDQPYTFLYYTNYHRAMPKNMKGYVFHPKEDFYKAEDWWLDQK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=513, Percent_Identity=26.9005847953216, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787052, Length=499, Percent_Identity=27.0541082164329, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787762, Length=499, Percent_Identity=24.6492985971944, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1787551, Length=553, Percent_Identity=22.4231464737794, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789887, Length=414, Percent_Identity=25.8454106280193, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI87082063, Length=459, Percent_Identity=23.0936819172113, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081878, Length=558, Percent_Identity=22.9390681003584, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789397, Length=541, Percent_Identity=21.4417744916821, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787495, Length=488, Percent_Identity=21.5163934426229, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62740; Mature: 62740

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRKTGFSILSLLLILSIFLTACNSGEVGGDEKEGKSDGKPQQGGDLIAGSTGEPTLFN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
SLYSTDTASSDIERLIYNTLLDVNEKLEVENQLADEVKESEDGLTFDVKLKEGVKFHDGE
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCC
EMTADDVVFTYSIPMSDEYVGERGSNFKMIESVTKKGKYEVQFKLKKQDPYFYNVTLASY
CCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEHHHC
GILPKHILKDVPISKLGEHEFNRKNPIGTGPFKFKEWKEGQYVKVEAFDDYYAGRPHLDS
CCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
ITYKIIPDSNAALSQLQAGDVDYLVVTPGPDYKTAEKFNNVKMETDLGLNYTYIGWNERN
EEEEEECCCCHHHHHHCCCCCCEEEECCCCCCHHHHHHCCEEEEECCCCEEEEECCCCHH
ELFKDKKVRQALTHALDRQALVDQVLDGDGEIANIPESPLSWNYPDNKDKFKTFEYDPEK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AKKLLKEAGWTDSDGDGILDKDGKKFSFVIKTNQGNKTREDLAVVVQQQLKEIGIQAKPQ
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
IVEWSALIEQMNPPNWDFDAMIMGWSLATFPDQSNIFHSKEAEKGLNYVWYQNEKLDKLL
EEHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCHHHHCCCCEEEECCCHHHHHH
DEAKTLKDREEYKKAYEDIYEILAEDQPYTFLYYTNYHRAMPKNMKGYVFHPKEDFYKAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCCCEEECCHHHCCCCC
DWWLDQK
CCCCCCC
>Mature Secondary Structure
MNKRKTGFSILSLLLILSIFLTACNSGEVGGDEKEGKSDGKPQQGGDLIAGSTGEPTLFN
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
SLYSTDTASSDIERLIYNTLLDVNEKLEVENQLADEVKESEDGLTFDVKLKEGVKFHDGE
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCCC
EMTADDVVFTYSIPMSDEYVGERGSNFKMIESVTKKGKYEVQFKLKKQDPYFYNVTLASY
CCCCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEHHHC
GILPKHILKDVPISKLGEHEFNRKNPIGTGPFKFKEWKEGQYVKVEAFDDYYAGRPHLDS
CCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
ITYKIIPDSNAALSQLQAGDVDYLVVTPGPDYKTAEKFNNVKMETDLGLNYTYIGWNERN
EEEEEECCCCHHHHHHCCCCCCEEEECCCCCCHHHHHHCCEEEEECCCCEEEEECCCCHH
ELFKDKKVRQALTHALDRQALVDQVLDGDGEIANIPESPLSWNYPDNKDKFKTFEYDPEK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AKKLLKEAGWTDSDGDGILDKDGKKFSFVIKTNQGNKTREDLAVVVQQQLKEIGIQAKPQ
HHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
IVEWSALIEQMNPPNWDFDAMIMGWSLATFPDQSNIFHSKEAEKGLNYVWYQNEKLDKLL
EEHHHHHHHHCCCCCCCCCEEEECCEEECCCCCCCCCCCHHHHCCCCEEEECCCHHHHHH
DEAKTLKDREEYKKAYEDIYEILAEDQPYTFLYYTNYHRAMPKNMKGYVFHPKEDFYKAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCCCEEECCHHHCCCCC
DWWLDQK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]