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The map label for this gene is yisK [H]

Identifier: 52784942

GI number: 52784942

Start: 1177560

End: 1178465

Strand: Direct

Name: yisK [H]

Synonym: BLi01170

Alternate gene names: 52784942

Gene position: 1177560-1178465 (Clockwise)

Preceding gene: 52784941

Following gene: 52784943

Centisome position: 27.89

GC content: 49.12

Gene sequence:

>906_bases
ATGAAGTTTGCGACAGCAAAGCTGCACAGCCGTACATTTGTAGGGCTTGTCATGGATGAGAAGATAATGGATTTGCAAAA
GGCGGAAAAAAAGCTGTTTGAGCTTGAAACGATACCTGAAACGCTTATCGAATGCGTAAGCGAGGGAGAGAAGTTTGTCC
GCCATGTCGAACAGCTAGTGGAATGGTCAAAGAAGAAAAACAGCGAAGAGAGCGGTTCGTATATTTATCCGCTGTCAGAC
GTCGATTTGCTTGCACCGATTCCCGAACCGCGGAAAAATGTGATCTGCATCGGAAAAAATTATCGGGATCATGCGATTGA
AATGGGAAGTGAAGCAGATATTCCCGAACATATCATGGTGTTTACAAAAGCGCCGACTTCGGTCATCGGCCACATGGAAG
GGATTGATGCCCACGAAGGCGTGACAGAGGCGCTCGATTATGAAGGCGAGCTCGCGGTTGTCATCGGCAAAACGGGAAAA
AACATCCCGAAAGCTGAAGCGCTCGACTATGTGTTCGGCTATACGATCATTAACGATGTGACGGCGCGCGATCTGCAAAA
GAGGCACAAGCAGTTTTTTATCGGCAAAAGCCTTGATGCGACCTGCCCGATGGGGCCGTTTCTTGTACATAAATCAATGA
TTGAAGACCCCGGTGACCTGAAGGTGGAGACGAGAGTGAACGGCGAACTCCGCCAGTCGGGGAGCACAAAGGACATGATT
TTTCCGATTGCGGAAATGATCGAAACGCTGTCCAAAGGAATGACGCTTGAAGCGGGGGATATTATCGCAACCGGTACGCC
GTCAGGAGTCGGCAAAGGCTTTAATCCGCCGAAGTTCCTCCGAAAAGGCGATCAGGTCGACATTACGATCGAACCGATCG
GAACGCTTTCCAATCCAGTTAAATAA

Upstream 100 bases:

>100_bases
GCCCGGGACTTTAGAAGCGGTTTTCTGAAAGAGGAAAGGAATTTTCGTTTTTTTAGAGAATATGTAGAATAGCTTTAAAT
CATACTGCGGGGGAATATTT

Downstream 100 bases:

>100_bases
AGGATTGTCAAAAGGATGGCTTTAGCATATAATGAAGCTGTCAAATTTTTTAGAAATGAGGGATGTGTGATGAGAATGGA
TATTGTCTTTTTTGCCCAAC

Product: YisK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKFATAKLHSRTFVGLVMDEKIMDLQKAEKKLFELETIPETLIECVSEGEKFVRHVEQLVEWSKKKNSEESGSYIYPLSD
VDLLAPIPEPRKNVICIGKNYRDHAIEMGSEADIPEHIMVFTKAPTSVIGHMEGIDAHEGVTEALDYEGELAVVIGKTGK
NIPKAEALDYVFGYTIINDVTARDLQKRHKQFFIGKSLDATCPMGPFLVHKSMIEDPGDLKVETRVNGELRQSGSTKDMI
FPIAEMIETLSKGMTLEAGDIIATGTPSGVGKGFNPPKFLRKGDQVDITIEPIGTLSNPVK

Sequences:

>Translated_301_residues
MKFATAKLHSRTFVGLVMDEKIMDLQKAEKKLFELETIPETLIECVSEGEKFVRHVEQLVEWSKKKNSEESGSYIYPLSD
VDLLAPIPEPRKNVICIGKNYRDHAIEMGSEADIPEHIMVFTKAPTSVIGHMEGIDAHEGVTEALDYEGELAVVIGKTGK
NIPKAEALDYVFGYTIINDVTARDLQKRHKQFFIGKSLDATCPMGPFLVHKSMIEDPGDLKVETRVNGELRQSGSTKDMI
FPIAEMIETLSKGMTLEAGDIIATGTPSGVGKGFNPPKFLRKGDQVDITIEPIGTLSNPVK
>Mature_301_residues
MKFATAKLHSRTFVGLVMDEKIMDLQKAEKKLFELETIPETLIECVSEGEKFVRHVEQLVEWSKKKNSEESGSYIYPLSD
VDLLAPIPEPRKNVICIGKNYRDHAIEMGSEADIPEHIMVFTKAPTSVIGHMEGIDAHEGVTEALDYEGELAVVIGKTGK
NIPKAEALDYVFGYTIINDVTARDLQKRHKQFFIGKSLDATCPMGPFLVHKSMIEDPGDLKVETRVNGELRQSGSTKDMI
FPIAEMIETLSKGMTLEAGDIIATGTPSGVGKGFNPPKFLRKGDQVDITIEPIGTLSNPVK

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=266, Percent_Identity=39.0977443609023, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI40786394, Length=266, Percent_Identity=38.3458646616541, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI66348062, Length=184, Percent_Identity=45.6521739130435, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI13654274, Length=184, Percent_Identity=45.6521739130435, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI215422413, Length=184, Percent_Identity=45.6521739130435, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1787428, Length=206, Percent_Identity=34.4660194174757, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557057, Length=183, Percent_Identity=42.0765027322404, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=231, Percent_Identity=29.004329004329, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28572127, Length=281, Percent_Identity=38.4341637010676, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24663695, Length=221, Percent_Identity=38.0090497737557, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28571789, Length=183, Percent_Identity=36.0655737704918, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 33277; Mature: 33277

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFATAKLHSRTFVGLVMDEKIMDLQKAEKKLFELETIPETLIECVSEGEKFVRHVEQLV
CCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EWSKKKNSEESGSYIYPLSDVDLLAPIPEPRKNVICIGKNYRDHAIEMGSEADIPEHIMV
HHHHHCCCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCEEEE
FTKAPTSVIGHMEGIDAHEGVTEALDYEGELAVVIGKTGKNIPKAEALDYVFGYTIINDV
EECCCHHHHHHCCCCCHHCCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
TARDLQKRHKQFFIGKSLDATCPMGPFLVHKSMIEDPGDLKVETRVNGELRQSGSTKDMI
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHH
FPIAEMIETLSKGMTLEAGDIIATGTPSGVGKGFNPPKFLRKGDQVDITIEPIGTLSNPV
HHHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCC
K
C
>Mature Secondary Structure
MKFATAKLHSRTFVGLVMDEKIMDLQKAEKKLFELETIPETLIECVSEGEKFVRHVEQLV
CCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EWSKKKNSEESGSYIYPLSDVDLLAPIPEPRKNVICIGKNYRDHAIEMGSEADIPEHIMV
HHHHHCCCCCCCCEEEECCCCCEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCEEEE
FTKAPTSVIGHMEGIDAHEGVTEALDYEGELAVVIGKTGKNIPKAEALDYVFGYTIINDV
EECCCHHHHHHCCCCCHHCCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
TARDLQKRHKQFFIGKSLDATCPMGPFLVHKSMIEDPGDLKVETRVNGELRQSGSTKDMI
HHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHH
FPIAEMIETLSKGMTLEAGDIIATGTPSGVGKGFNPPKFLRKGDQVDITIEPIGTLSNPV
HHHHHHHHHHHCCCEEECCCEEECCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]