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The map label for this gene is pucH

Identifier: 52784898

GI number: 52784898

Start: 1128281

End: 1129645

Strand: Direct

Name: pucH

Synonym: BLi01126

Alternate gene names: 52784898

Gene position: 1128281-1129645 (Clockwise)

Preceding gene: 52784896

Following gene: 52784899

Centisome position: 26.72

GC content: 39.34

Gene sequence:

>1365_bases
ATGAATTTTGATTCAATTATCAAAAATGGATTAGTCATTCTAGAAAATGGTGAACAAGAAGTCGAAGTAGGGATCAAAGA
TGGCAAAATCGCAGCAATCGGTCAGGATTTGGGTACAAGTGCAAAAATTATCGATGCAAAAGGATCTATTGTATCTCCAG
GTATGATTGATGCACATGTGCATATCACAGAGCCGGGCGGCGGCTATCGTGATGAATGGGAGGGCTATGACACAGGAACA
CGTGCCGCTGCAAAAGGCGGTGTAACGACGTTTATTGAAATGCCATTAAATCAAGTGCCAGCAACGGTAGATGAGGAATC
TATTCAAGAAAAATTTAAAGCCGGAAAAGGAAAATTATCAGTAGACGTTGCGAGTTACGGCGGTTTAGTACCATTTGACT
TAGATGGGGGTATTCAAGAATTAGATAGCAATGGTGTTGTTGCATATAAATGTTTTTTAGCAACATGTGGCGACCGTTCT
ATAGAAGGAGATTTCATGAACGTAGATGACTATTCCCTCTACGAAGGCATGAAACAAATTGCGAAGACAGGCAAAATATT
ATCTATACACGCTGAAAATGCCGCAATTACCGATAAGTTAGGGGAAATAGCATCTAAAAATGGGGAAACTTCATTGCGTG
CTTATGTAGATTCTCGACCCGTATTTACTGAAGTAGAACCGATTAGAAAAATTATTCTATTTGCGAAAGAAACGGGATGT
CGTGTACACATCGTTCATATAGCTTGTGAAGAGGGCGTAGACGAAATTGTCAAAGCACAACAGGAAGGGGTCGATATTAC
ATGCGAAACATGTACACACTATTTATATTTCTATAAAGAAGAACTAGATCATATCGGACCGGTTGTAAAATGCTCTCCGC
CAATCCGTGAGCAATCACGCTTAGAAGGCATGTGGAATCGGGTATTAAACGGAGATATTTCATTCGTAACATCAGACCAT
TCACCATGTACCCCGGACTTAAAAGCTACGGATAATGCATTTGAAGCTTGGGGAGGTATCGCAGGTTTACAAAATAATGT
CGATGTATTATTTGATGAAGGTGTGCAAAAACGTAATATGCCATTATCCAAATTTGCAGCCATTATTGCTACAAACCCTG
CGAAACGTTTTAATCTCGCATCAAAAGGCAGTATTGCAGTAGGGAAAGATGCGGATTTCGTATTTATTAAGAAAGATGCA
CCTTATGAATTAAAGGCTGAGGACTTAGCATATCGACACAAAATTAGTCCGTATATTGGCCGCAAAATTGGTGCTCAGGT
AGTGAAAACAATCCTTCGCGGTGTCGAAATCTATGATAAAGAAAAAGGAATTTCAAATGAAAAAGTAGGAAGATTTATTC
CTTAA

Upstream 100 bases:

>100_bases
GCGGTTTCCTTGCTACAGGTGTACTTCTATTTACAGAAATCTCTATCAATGCAGCAAGTAATATTATTCTTGCAGCTTAA
AAAAGAGGAGGATGATTTTG

Downstream 100 bases:

>100_bases
GTGACGGAATAGCCATCCCGTCAGGAATTTGAGGCATAGGAAGATGTGAAGGCGACCTGAGATTGTGTTACACTCGCAAA
TCAGTGTGTCATCAGCATTT

Product: allantoinase

Products: NA

Alternate protein names: Allantoin-utilizing enzyme

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNFDSIIKNGLVILENGEQEVEVGIKDGKIAAIGQDLGTSAKIIDAKGSIVSPGMIDAHVHITEPGGGYRDEWEGYDTGT
RAAAKGGVTTFIEMPLNQVPATVDEESIQEKFKAGKGKLSVDVASYGGLVPFDLDGGIQELDSNGVVAYKCFLATCGDRS
IEGDFMNVDDYSLYEGMKQIAKTGKILSIHAENAAITDKLGEIASKNGETSLRAYVDSRPVFTEVEPIRKIILFAKETGC
RVHIVHIACEEGVDEIVKAQQEGVDITCETCTHYLYFYKEELDHIGPVVKCSPPIREQSRLEGMWNRVLNGDISFVTSDH
SPCTPDLKATDNAFEAWGGIAGLQNNVDVLFDEGVQKRNMPLSKFAAIIATNPAKRFNLASKGSIAVGKDADFVFIKKDA
PYELKAEDLAYRHKISPYIGRKIGAQVVKTILRGVEIYDKEKGISNEKVGRFIP

Sequences:

>Translated_454_residues
MNFDSIIKNGLVILENGEQEVEVGIKDGKIAAIGQDLGTSAKIIDAKGSIVSPGMIDAHVHITEPGGGYRDEWEGYDTGT
RAAAKGGVTTFIEMPLNQVPATVDEESIQEKFKAGKGKLSVDVASYGGLVPFDLDGGIQELDSNGVVAYKCFLATCGDRS
IEGDFMNVDDYSLYEGMKQIAKTGKILSIHAENAAITDKLGEIASKNGETSLRAYVDSRPVFTEVEPIRKIILFAKETGC
RVHIVHIACEEGVDEIVKAQQEGVDITCETCTHYLYFYKEELDHIGPVVKCSPPIREQSRLEGMWNRVLNGDISFVTSDH
SPCTPDLKATDNAFEAWGGIAGLQNNVDVLFDEGVQKRNMPLSKFAAIIATNPAKRFNLASKGSIAVGKDADFVFIKKDA
PYELKAEDLAYRHKISPYIGRKIGAQVVKTILRGVEIYDKEKGISNEKVGRFIP
>Mature_454_residues
MNFDSIIKNGLVILENGEQEVEVGIKDGKIAAIGQDLGTSAKIIDAKGSIVSPGMIDAHVHITEPGGGYRDEWEGYDTGT
RAAAKGGVTTFIEMPLNQVPATVDEESIQEKFKAGKGKLSVDVASYGGLVPFDLDGGIQELDSNGVVAYKCFLATCGDRS
IEGDFMNVDDYSLYEGMKQIAKTGKILSIHAENAAITDKLGEIASKNGETSLRAYVDSRPVFTEVEPIRKIILFAKETGC
RVHIVHIACEEGVDEIVKAQQEGVDITCETCTHYLYFYKEELDHIGPVVKCSPPIREQSRLEGMWNRVLNGDISFVTSDH
SPCTPDLKATDNAFEAWGGIAGLQNNVDVLFDEGVQKRNMPLSKFAAIIATNPAKRFNLASKGSIAVGKDADFVFIKKDA
PYELKAEDLAYRHKISPYIGRKIGAQVVKTILRGVEIYDKEKGISNEKVGRFIP

Specific function: Catalyzes the conversion of allantoin (5- ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Allantoinase subfamily

Homologues:

Organism=Homo sapiens, GI19923821, Length=472, Percent_Identity=27.1186440677966, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4503375, Length=477, Percent_Identity=27.2536687631027, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4503379, Length=466, Percent_Identity=25.7510729613734, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI4503051, Length=466, Percent_Identity=26.3948497854077, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI62422571, Length=466, Percent_Identity=26.3948497854077, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503377, Length=465, Percent_Identity=25.1612903225806, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI190194363, Length=464, Percent_Identity=24.1379310344828, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786722, Length=453, Percent_Identity=55.1876379690949, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI87082175, Length=470, Percent_Identity=25.9574468085106, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17539558, Length=471, Percent_Identity=25.0530785562633, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71989490, Length=477, Percent_Identity=25.7861635220126, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI193204318, Length=387, Percent_Identity=24.8062015503876, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322218, Length=409, Percent_Identity=34.4743276283619, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI18859883, Length=422, Percent_Identity=31.7535545023697, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI221377917, Length=481, Percent_Identity=24.7401247401247, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17137462, Length=469, Percent_Identity=24.3070362473348, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24642586, Length=408, Percent_Identity=23.7745098039216, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24644287, Length=319, Percent_Identity=25.0783699059561, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALLB_BACLD (Q65LN0)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078324.1
- RefSeq:   YP_090727.1
- HSSP:   P81006
- ProteinModelPortal:   Q65LN0
- STRING:   Q65LN0
- EnsemblBacteria:   EBBACT00000055868
- EnsemblBacteria:   EBBACT00000060224
- GeneID:   3030063
- GeneID:   3098142
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01126
- KEGG:   bli:BL01094
- NMPDR:   fig|279010.5.peg.2116
- eggNOG:   COG0044
- GeneTree:   EBGT00050000001626
- HOGENOM:   HBG724623
- OMA:   DITCETC
- ProtClustDB:   PRK08044
- BioCyc:   BLIC279010-1:BLI01126-MONOMER
- BioCyc:   BLIC279010:BL01094-MONOMER
- HAMAP:   MF_01645
- InterPro:   IPR017593
- InterPro:   IPR006680
- InterPro:   IPR011059
- TIGRFAMs:   TIGR03178

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.5

Molecular weight: Translated: 49489; Mature: 49489

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDSIIKNGLVILENGEQEVEVGIKDGKIAAIGQDLGTSAKIIDAKGSIVSPGMIDAHV
CCHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCEECCCEEEEEE
HITEPGGGYRDEWEGYDTGTRAAAKGGVTTFIEMPLNQVPATVDEESIQEKFKAGKGKLS
EEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCCCEEE
VDVASYGGLVPFDLDGGIQELDSNGVVAYKCFLATCGDRSIEGDFMNVDDYSLYEGMKQI
EEECCCCCEEEEECCCCHHHHCCCCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHH
AKTGKILSIHAENAAITDKLGEIASKNGETSLRAYVDSRPVFTEVEPIRKIILFAKETGC
HHCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCC
RVHIVHIACEEGVDEIVKAQQEGVDITCETCTHYLYFYKEELDHIGPVVKCSPPIREQSR
EEEEEEEEECCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHH
LEGMWNRVLNGDISFVTSDHSPCTPDLKATDNAFEAWGGIAGLQNNVDVLFDEGVQKRNM
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHCC
PLSKFAAIIATNPAKRFNLASKGSIAVGKDADFVFIKKDAPYELKAEDLAYRHKISPYIG
CHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHH
RKIGAQVVKTILRGVEIYDKEKGISNEKVGRFIP
HHHHHHHHHHHHHCCEEEHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNFDSIIKNGLVILENGEQEVEVGIKDGKIAAIGQDLGTSAKIIDAKGSIVSPGMIDAHV
CCHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCEECCCEEEEEE
HITEPGGGYRDEWEGYDTGTRAAAKGGVTTFIEMPLNQVPATVDEESIQEKFKAGKGKLS
EEECCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCCCEEE
VDVASYGGLVPFDLDGGIQELDSNGVVAYKCFLATCGDRSIEGDFMNVDDYSLYEGMKQI
EEECCCCCEEEEECCCCHHHHCCCCEEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHH
AKTGKILSIHAENAAITDKLGEIASKNGETSLRAYVDSRPVFTEVEPIRKIILFAKETGC
HHCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCC
RVHIVHIACEEGVDEIVKAQQEGVDITCETCTHYLYFYKEELDHIGPVVKCSPPIREQSR
EEEEEEEEECCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHH
LEGMWNRVLNGDISFVTSDHSPCTPDLKATDNAFEAWGGIAGLQNNVDVLFDEGVQKRNM
HHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCHHCC
PLSKFAAIIATNPAKRFNLASKGSIAVGKDADFVFIKKDAPYELKAEDLAYRHKISPYIG
CHHHHHHHHCCCCHHHCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHH
RKIGAQVVKTILRGVEIYDKEKGISNEKVGRFIP
HHHHHHHHHHHHHCCEEEHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA