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The map label for this gene is pbpF [H]

Identifier: 52784863

GI number: 52784863

Start: 1088717

End: 1090855

Strand: Direct

Name: pbpF [H]

Synonym: BLi01091

Alternate gene names: 52784863

Gene position: 1088717-1090855 (Clockwise)

Preceding gene: 52784859

Following gene: 52784864

Centisome position: 25.78

GC content: 47.45

Gene sequence:

>2139_bases
ATGAGAAAGAGATTTTTCATTCCAATCATCATTACAGCGGCGATATTCCTTTTATCGTTAATCGGATATATTACGATTTT
GTTCCTGGGGGATTATGTGATTGATGAAAAGAAACTGATTTTCCATGCTTCTTCTAAAATCGTCGACCATAATGGAGAAG
AAGTCGCAAGCCTCTATACCGAAAACAGAAGGCCTGTTTCAATTGCCGAGGTGCCGGACAATGTAAAAAATGCCTTTATC
GCGGTTGAAGACAAGCGCTTTTATGAGCACCACGGCATCGATTTTAAATCGGTTAGCCGTGCGGTCTATAAAGATATATT
AGCAGGAGGAAAAGTGGAAGGGGGAAGCACGATCACCCAGCAGCTCGCAAAAAACACTTTTTTGACAAATGACAAAACGT
TTTTAAGAAAAACGAAAGAAGTCATTATCGCTATCAATTTGGAGCGGGATTACAGCAAAGACAAGCTTTTGGAAATGTAT
TTGAACCAGCTATATTTCGGGCACGGCGTCTATGGAATCCAGGCGGCGGCAAACTACTTTTTTAACAAAGATGTGAAAGA
CTTGACGGTCAGTGAGGGAGCGGTGCTGGCTTCCATTCCTAAAGCGCCTTCAACGTATTCGCCCATTCTCAATCCGGAAA
AAAGCCGGGAGCGGCGCAATGTCATTCTCGGCATGATGAACGAGCAGGGATATATCAGCTCAAAGGAAACCGTCAGGGCC
CAAGGCAGCACATTGGGGCTCCATGTGAAAGAAAAATCAAAAAATCCGTGGCTTGACAGCTATATTGATCTTGTCATGAA
AGAAGCGGAAGACAAATATTCGATATCGAGCGAACAGCTTCTCCAAGGCGGATACACGATCAGTGTTCCGCTCGATGTTT
CGATTCAAAAAGCGGCCTACACGCTGATGAAGCAGGACGGCTATTTTCCCGGCACAGACAACAAAGCTGAAGGGAGCGCC
GTTTTTATCGATAACAGGACAGGCGGCGTAGTAGCCGCTCTCGGGGGACGGGAATATGCTCCAAAAGGCTATAACAGGGC
CACCGCCCAGCGCCAGCCGGGTTCGACGTTTAAACCGCTTGCCGTATACGGCCCCGCTCTTGAAGAAAAGCTGTTCAAGC
CGTATTCGATGCTCGAGGATAAACAGCTGTCATACGGCGGCTACTCGCCTAAAAACTATGACGGCCAATACGCGGGCAAG
GTCTCGATGGTGGATGCGCTGACATACAGCAAAAACGCGCCTGCTGTATGGACGTTAAATGAGATCGGAATCGAAACGGT
GAAGCCTTATCTCAGCCGTATGGGCATGAACGTGCCGGATGAAGGGCTGGCATTGGGGCTAGGCGGTCTGGAAAAAGGGG
TGTCGCCGCTCGAGCTTGCCGGGGCATACCGGACATTCGCAAATTCAGGACAATACACCGAGCCGTTTTTTATTAAAAAA
ATCACCGATGAAAACGGGGATGTCATGTTCCGCCACGAGGAAAAATCGGAAAAGGTTTTCAGCAAACAAACAGCGTGGAA
CATGACGCGGATGCTGGAGGAGGTCGTGAAAAGCGGAACGGGAAAGGCCGGTGATTTTTCCGGGGAGATTGCAGGAAAAA
CGGGTTCGACCACGTATACCGGAAAAGAGGGCGGAACTAAAGACGCCTGGTTTGCGGGGTATACGCCCGAAGTGACGGGA
GCTGTCTGGATGGGATACGATAAAACGGATGAGTCCCATTATTTAAAAGGCGGCAGCGCTTATCCGACAAGGCTGTTTAA
AGACATTTTAAAACGCACCAACCATAAGCCTGAACGGTTTAAGGCGCCTGAAGGGGTAAAAGATCTGGACAGGCCGATAC
GCCTCGAAGACCTGGAAAGCGTTAAAGCAGACTATTCCTTTACGCCGATGGGGCTGATTACGGTGTCATTAAAATGGCAT
GAACAAGAGGATAAAAGGGTTGAATACCGAATATATGAAAGGAAAAACGGAAAAGAAACGCTGATTGATACGGTTAAGGG
AAAAGGAAGCTTTTCAATCCCTTATGCAAACATCTTTTCCGATGCTTCCTACAAAATTGTTCCATACAATCCGCAGACGA
AAACAGAGGGAAAAGGGACTGATTATGTTGAACCGAAAGTGTTTCCTTCGGGTCATTAG

Upstream 100 bases:

>100_bases
AGGACGATTTAAAATTCTATCCATTACCCGCTATAAATGCTGCAACATAATTTGAAGAAAAAATTCCCATACTAGCTATA
GAAAGGAATGAGGTGACCAT

Downstream 100 bases:

>100_bases
ATACCCGAAATTCATCAATTTCTTGAACAATAAGTCTCATGAGTATACCGTTCAATAAAAAAACGGTATATTGGAAGGAG
TAATTTTGCGAACGTATGGT

Product: PbpF

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MRKRFFIPIIITAAIFLLSLIGYITILFLGDYVIDEKKLIFHASSKIVDHNGEEVASLYTENRRPVSIAEVPDNVKNAFI
AVEDKRFYEHHGIDFKSVSRAVYKDILAGGKVEGGSTITQQLAKNTFLTNDKTFLRKTKEVIIAINLERDYSKDKLLEMY
LNQLYFGHGVYGIQAAANYFFNKDVKDLTVSEGAVLASIPKAPSTYSPILNPEKSRERRNVILGMMNEQGYISSKETVRA
QGSTLGLHVKEKSKNPWLDSYIDLVMKEAEDKYSISSEQLLQGGYTISVPLDVSIQKAAYTLMKQDGYFPGTDNKAEGSA
VFIDNRTGGVVAALGGREYAPKGYNRATAQRQPGSTFKPLAVYGPALEEKLFKPYSMLEDKQLSYGGYSPKNYDGQYAGK
VSMVDALTYSKNAPAVWTLNEIGIETVKPYLSRMGMNVPDEGLALGLGGLEKGVSPLELAGAYRTFANSGQYTEPFFIKK
ITDENGDVMFRHEEKSEKVFSKQTAWNMTRMLEEVVKSGTGKAGDFSGEIAGKTGSTTYTGKEGGTKDAWFAGYTPEVTG
AVWMGYDKTDESHYLKGGSAYPTRLFKDILKRTNHKPERFKAPEGVKDLDRPIRLEDLESVKADYSFTPMGLITVSLKWH
EQEDKRVEYRIYERKNGKETLIDTVKGKGSFSIPYANIFSDASYKIVPYNPQTKTEGKGTDYVEPKVFPSGH

Sequences:

>Translated_712_residues
MRKRFFIPIIITAAIFLLSLIGYITILFLGDYVIDEKKLIFHASSKIVDHNGEEVASLYTENRRPVSIAEVPDNVKNAFI
AVEDKRFYEHHGIDFKSVSRAVYKDILAGGKVEGGSTITQQLAKNTFLTNDKTFLRKTKEVIIAINLERDYSKDKLLEMY
LNQLYFGHGVYGIQAAANYFFNKDVKDLTVSEGAVLASIPKAPSTYSPILNPEKSRERRNVILGMMNEQGYISSKETVRA
QGSTLGLHVKEKSKNPWLDSYIDLVMKEAEDKYSISSEQLLQGGYTISVPLDVSIQKAAYTLMKQDGYFPGTDNKAEGSA
VFIDNRTGGVVAALGGREYAPKGYNRATAQRQPGSTFKPLAVYGPALEEKLFKPYSMLEDKQLSYGGYSPKNYDGQYAGK
VSMVDALTYSKNAPAVWTLNEIGIETVKPYLSRMGMNVPDEGLALGLGGLEKGVSPLELAGAYRTFANSGQYTEPFFIKK
ITDENGDVMFRHEEKSEKVFSKQTAWNMTRMLEEVVKSGTGKAGDFSGEIAGKTGSTTYTGKEGGTKDAWFAGYTPEVTG
AVWMGYDKTDESHYLKGGSAYPTRLFKDILKRTNHKPERFKAPEGVKDLDRPIRLEDLESVKADYSFTPMGLITVSLKWH
EQEDKRVEYRIYERKNGKETLIDTVKGKGSFSIPYANIFSDASYKIVPYNPQTKTEGKGTDYVEPKVFPSGH
>Mature_712_residues
MRKRFFIPIIITAAIFLLSLIGYITILFLGDYVIDEKKLIFHASSKIVDHNGEEVASLYTENRRPVSIAEVPDNVKNAFI
AVEDKRFYEHHGIDFKSVSRAVYKDILAGGKVEGGSTITQQLAKNTFLTNDKTFLRKTKEVIIAINLERDYSKDKLLEMY
LNQLYFGHGVYGIQAAANYFFNKDVKDLTVSEGAVLASIPKAPSTYSPILNPEKSRERRNVILGMMNEQGYISSKETVRA
QGSTLGLHVKEKSKNPWLDSYIDLVMKEAEDKYSISSEQLLQGGYTISVPLDVSIQKAAYTLMKQDGYFPGTDNKAEGSA
VFIDNRTGGVVAALGGREYAPKGYNRATAQRQPGSTFKPLAVYGPALEEKLFKPYSMLEDKQLSYGGYSPKNYDGQYAGK
VSMVDALTYSKNAPAVWTLNEIGIETVKPYLSRMGMNVPDEGLALGLGGLEKGVSPLELAGAYRTFANSGQYTEPFFIKK
ITDENGDVMFRHEEKSEKVFSKQTAWNMTRMLEEVVKSGTGKAGDFSGEIAGKTGSTTYTGKEGGTKDAWFAGYTPEVTG
AVWMGYDKTDESHYLKGGSAYPTRLFKDILKRTNHKPERFKAPEGVKDLDRPIRLEDLESVKADYSFTPMGLITVSLKWH
EQEDKRVEYRIYERKNGKETLIDTVKGKGSFSIPYANIFSDASYKIVPYNPQTKTEGKGTDYVEPKVFPSGH

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=583, Percent_Identity=30.3602058319039, Blast_Score=225, Evalue=7e-60,
Organism=Escherichia coli, GI87082258, Length=274, Percent_Identity=37.956204379562, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788867, Length=611, Percent_Identity=28.6415711947627, Blast_Score=189, Evalue=7e-49,
Organism=Escherichia coli, GI1789601, Length=231, Percent_Identity=29.8701298701299, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79409; Mature: 79409

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRFFIPIIITAAIFLLSLIGYITILFLGDYVIDEKKLIFHASSKIVDHNGEEVASLYT
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
ENRRPVSIAEVPDNVKNAFIAVEDKRFYEHHGIDFKSVSRAVYKDILAGGKVEGGSTITQ
CCCCCEEHHCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
QLAKNTFLTNDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAAANYF
HHHHHCCCCCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FNKDVKDLTVSEGAVLASIPKAPSTYSPILNPEKSRERRNVILGMMNEQGYISSKETVRA
HCCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCHHHEE
QGSTLGLHVKEKSKNPWLDSYIDLVMKEAEDKYSISSEQLLQGGYTISVPLDVSIQKAAY
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHCCCCHHHHHHCCEEEEEECCCCHHHHHH
TLMKQDGYFPGTDNKAEGSAVFIDNRTGGVVAALGGREYAPKGYNRATAQRQPGSTFKPL
HHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AVYGPALEEKLFKPYSMLEDKQLSYGGYSPKNYDGQYAGKVSMVDALTYSKNAPAVWTLN
EEECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHEEECCCCCEEEEHH
EIGIETVKPYLSRMGMNVPDEGLALGLGGLEKGVSPLELAGAYRTFANSGQYTEPFFIKK
HHCHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEE
ITDENGDVMFRHEEKSEKVFSKQTAWNMTRMLEEVVKSGTGKAGDFSGEIAGKTGSTTYT
EECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEE
GKEGGTKDAWFAGYTPEVTGAVWMGYDKTDESHYLKGGSAYPTRLFKDILKRTNHKPERF
CCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCHHC
KAPEGVKDLDRPIRLEDLESVKADYSFTPMGLITVSLKWHEQEDKRVEYRIYERKNGKET
CCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEEECCCCCHH
LIDTVKGKGSFSIPYANIFSDASYKIVPYNPQTKTEGKGTDYVEPKVFPSGH
HHHHHCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKRFFIPIIITAAIFLLSLIGYITILFLGDYVIDEKKLIFHASSKIVDHNGEEVASLYT
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHH
ENRRPVSIAEVPDNVKNAFIAVEDKRFYEHHGIDFKSVSRAVYKDILAGGKVEGGSTITQ
CCCCCEEHHCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
QLAKNTFLTNDKTFLRKTKEVIIAINLERDYSKDKLLEMYLNQLYFGHGVYGIQAAANYF
HHHHHCCCCCCHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FNKDVKDLTVSEGAVLASIPKAPSTYSPILNPEKSRERRNVILGMMNEQGYISSKETVRA
HCCCCCCEEECCCEEEEECCCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCCCCHHHEE
QGSTLGLHVKEKSKNPWLDSYIDLVMKEAEDKYSISSEQLLQGGYTISVPLDVSIQKAAY
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCHHCCCCHHHHHHCCEEEEEECCCCHHHHHH
TLMKQDGYFPGTDNKAEGSAVFIDNRTGGVVAALGGREYAPKGYNRATAQRQPGSTFKPL
HHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
AVYGPALEEKLFKPYSMLEDKQLSYGGYSPKNYDGQYAGKVSMVDALTYSKNAPAVWTLN
EEECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEHEEECCCCCEEEEHH
EIGIETVKPYLSRMGMNVPDEGLALGLGGLEKGVSPLELAGAYRTFANSGQYTEPFFIKK
HHCHHHHHHHHHHCCCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEE
ITDENGDVMFRHEEKSEKVFSKQTAWNMTRMLEEVVKSGTGKAGDFSGEIAGKTGSTTYT
EECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEE
GKEGGTKDAWFAGYTPEVTGAVWMGYDKTDESHYLKGGSAYPTRLFKDILKRTNHKPERF
CCCCCCCCCEECCCCCCCCCEEEECCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCHHC
KAPEGVKDLDRPIRLEDLESVKADYSFTPMGLITVSLKWHEQEDKRVEYRIYERKNGKET
CCCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEEECCCCCHH
LIDTVKGKGSFSIPYANIFSDASYKIVPYNPQTKTEGKGTDYVEPKVFPSGH
HHHHHCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]