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The map label for this gene is gatB [H]

Identifier: 52784531

GI number: 52784531

Start: 737046

End: 738476

Strand: Direct

Name: gatB [H]

Synonym: BLi00732

Alternate gene names: 52784531

Gene position: 737046-738476 (Clockwise)

Preceding gene: 52784530

Following gene: 52784532

Centisome position: 17.45

GC content: 49.62

Gene sequence:

>1431_bases
ATGAACTTTGAAACGGTAATCGGACTTGAAGTCCACGTGGAGCTCAAAACAAAATCGAAAATTTTCTCCAGTTCTCCAAC
GCCATTCGGCGCGGCGGCCAACACGCAGACAAGCGTTATCGACTTAGGCTATCCAGGTGTACTGCCTGTGTTGAACAAAG
AAGCCGTCAATTTCGCGATGAAAGCCGCAATGGCGCTCAACTGCGAAATTGCGACAGATACGAAATTCGACCGGAAAAAC
TATTTCTACCCCGACAACCCGAAAGCTTATCAAATTTCACAATTTGATAAACCGATCGGCGAAAACGGCTGGATCGAAAT
CGAAGTCGGCGGAAAAACGAAGCGCATCGGCATTACCCGCCTTCATTTGGAAGAGGATGCGGGAAAACTGACGCATACAG
GCGACGGCTACTCCCTTGTCGACTATAACCGCCAGGGAACACCGCTCGTTGAAATCGTCTCCGAGCCCGATATCAGAACG
CCTGAAGAAGCTTACGCCTACCTTGAAAAACTGAAATCAATCATTCAGTATACAGGCGTGTCCGATTGTAAGATGGAAGA
GGGGTCGCTCCGCTGCGACGCCAACATCTCGCTCCGTCCGATCGGACAAGAGGAGTTCGGTACAAAGACAGAGCTGAAAA
ACCTGAACTCATTCGCCTTTGTTCAAAAAGGCCTTGAGCACGAAGAAAAACGCCAGGCGCAGGTTCTTTTGTCAGGCGGT
GTCATTCAGCAGGAGACGCGCCGCTATGATGAAGCGTCCAAAACGACGATTTTGATGAGGGTCAAAGAGGGATCTGACGA
CTACCGCTACTTCCCTGAGCCTGATCTTGTCGAGCTTTACATAGACGACGAGTGGAAGCAACGCGTGAAAGACAGCATTC
CTGAGCTTCCGGACGAGCGCCGCAAGCGCTACATCGAAGAGCTTGGCCTCCCTGCCTATGACGCGAAAGTGCTGACTTTG
ACAAAAGAAATGTCCGACTTCTTCGAAGCGGCCGTTGAAAAAGGCGCAGACGCGAAGCTGGCGTCGAACTGGCTGATGGG
TGAAGTATCGGCTTACTTGAACGCCCAGCAAAAAGAGCTTGCGGATGTCGAACTCACACCTGAAGGCCTTGCCGGATTGG
TCAAGCTGATCGAAAAAGGAACGATTTCATCGAAAATCGCGAAAAAGGTCTTCAAAGAATTGATTGAAAAAGGCGGAGAC
GCAGAAAAAATCGTCAAAGAAAAAGGCCTTGTGCAAATCTCTGACGAAGCGACGCTCAGAAAGCTTGTCACAGAAGCGCT
CGACAACAATCCGCAATCAATCGAAGACTTTAAAAACGGAAAAGACCGCGCGATCGGCTTCCTTGTCGGCCAAATCATGA
AAGCCTCAAAAGGACAAGCGAACCCGCCGATGGTCAACAAGCTTCTTCTTGAAGAAATCAATAAACGATAA

Upstream 100 bases:

>100_bases
AAACACTTTGACGAAAGCACAGTATACCGCGTCGCCCACGCATTCGAACAGGCGACAGACCACCATAAAGCAAAACCTGA
ACTGTAAGGGGTGAAAAAAC

Downstream 100 bases:

>100_bases
AAAACAGACTATGCCTAATTAAATGGAGGTCCTCATGTTGAGGACCTTTTTTATTTGTATGAAAATACAAATGAAAAAAC
TTCTTTCTGTTCAATAATAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETVIGLEVHVELKTKSKIFSSSPTPFGAAANTQTSVIDLGYPGVLPVLNKEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITRLHLEEDAGKLTHTGDGYSLVDYNRQGTPLVEIVSEPDIRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQAQVLLSGG
VIQQETRRYDEASKTTILMRVKEGSDDYRYFPEPDLVELYIDDEWKQRVKDSIPELPDERRKRYIEELGLPAYDAKVLTL
TKEMSDFFEAAVEKGADAKLASNWLMGEVSAYLNAQQKELADVELTPEGLAGLVKLIEKGTISSKIAKKVFKELIEKGGD
AEKIVKEKGLVQISDEATLRKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKLLLEEINKR

Sequences:

>Translated_476_residues
MNFETVIGLEVHVELKTKSKIFSSSPTPFGAAANTQTSVIDLGYPGVLPVLNKEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITRLHLEEDAGKLTHTGDGYSLVDYNRQGTPLVEIVSEPDIRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQAQVLLSGG
VIQQETRRYDEASKTTILMRVKEGSDDYRYFPEPDLVELYIDDEWKQRVKDSIPELPDERRKRYIEELGLPAYDAKVLTL
TKEMSDFFEAAVEKGADAKLASNWLMGEVSAYLNAQQKELADVELTPEGLAGLVKLIEKGTISSKIAKKVFKELIEKGGD
AEKIVKEKGLVQISDEATLRKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKLLLEEINKR
>Mature_476_residues
MNFETVIGLEVHVELKTKSKIFSSSPTPFGAAANTQTSVIDLGYPGVLPVLNKEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITRLHLEEDAGKLTHTGDGYSLVDYNRQGTPLVEIVSEPDIRT
PEEAYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQAQVLLSGG
VIQQETRRYDEASKTTILMRVKEGSDDYRYFPEPDLVELYIDDEWKQRVKDSIPELPDERRKRYIEELGLPAYDAKVLTL
TKEMSDFFEAAVEKGADAKLASNWLMGEVSAYLNAQQKELADVELTPEGLAGLVKLIEKGTISSKIAKKVFKELIEKGGD
AEKIVKEKGLVQISDEATLRKLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKLLLEEINKR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=490, Percent_Identity=36.734693877551, Blast_Score=299, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=39.2739273927393, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=30.2, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=32.9896907216495, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53261; Mature: 53261

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELKTKSKIFSSSPTPFGAAANTQTSVIDLGYPGVLPVLNKEAVNFAM
CCCCEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCEEEEEECCCEEEEEEEE
LHLEEDAGKLTHTGDGYSLVDYNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKSIIQYTGV
EEEECCCCCCEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQAQVLLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCC
VIQQETRRYDEASKTTILMRVKEGSDDYRYFPEPDLVELYIDDEWKQRVKDSIPELPDER
CHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHH
RKRYIEELGLPAYDAKVLTLTKEMSDFFEAAVEKGADAKLASNWLMGEVSAYLNAQQKEL
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
ADVELTPEGLAGLVKLIEKGTISSKIAKKVFKELIEKGGDAEKIVKEKGLVQISDEATLR
CCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHH
KLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKLLLEEINKR
HHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETVIGLEVHVELKTKSKIFSSSPTPFGAAANTQTSVIDLGYPGVLPVLNKEAVNFAM
CCCCEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGWIEIEVGGKTKRIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCCEEEEEECCCEEEEEEEE
LHLEEDAGKLTHTGDGYSLVDYNRQGTPLVEIVSEPDIRTPEEAYAYLEKLKSIIQYTGV
EEEECCCCCCEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEEGSLRCDANISLRPIGQEEFGTKTELKNLNSFAFVQKGLEHEEKRQAQVLLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCC
VIQQETRRYDEASKTTILMRVKEGSDDYRYFPEPDLVELYIDDEWKQRVKDSIPELPDER
CHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCHHH
RKRYIEELGLPAYDAKVLTLTKEMSDFFEAAVEKGADAKLASNWLMGEVSAYLNAQQKEL
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
ADVELTPEGLAGLVKLIEKGTISSKIAKKVFKELIEKGGDAEKIVKEKGLVQISDEATLR
CCCEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHH
KLVTEALDNNPQSIEDFKNGKDRAIGFLVGQIMKASKGQANPPMVNKLLLEEINKR
HHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA