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The map label for this gene is gatA [H]

Identifier: 52784530

GI number: 52784530

Start: 735575

End: 737032

Strand: Direct

Name: gatA [H]

Synonym: BLi00731

Alternate gene names: 52784530

Gene position: 735575-737032 (Clockwise)

Preceding gene: 52784529

Following gene: 52784531

Centisome position: 17.42

GC content: 50.62

Gene sequence:

>1458_bases
ATGTCACTGTTTGACCATAAAATATCTGAATTAAAACGTCTCATACATAATAAAGAGATCAGCATATCAGATTTAGTCGA
TGAATCTTACAAACGGATTCACGAAGTTGACGGAAAGGTACAAGCCTTTTTGCAGCTTGATGAAGAAAAAGCCCGCGCAT
ATGCCAAAGAATTGGACGAAGCGCTTGATACCCGGGATGAGCACGGTCTTCTTTTCGGGATGCCGATCGGCATTAAGGAC
AATATCGTAACAAAAGATCTGCGGACAACGTGCGCAAGCAAAATTCTCGAGAATTTTGATCCGATCTATGATGCAACCGT
TGTTGAGCGTCTTCATGAAGCTGAAGCGGTTACCATCGGAAAGCTGAATATGGATGAATTCGCGATGGGCTCTTCCACAG
AGAACTCAGGCTTTAAAAAGACAAAAAACCCGTGGAACCTCGAAACTGTTCCTGGCGGATCAAGCGGAGGCTCAGCGGCT
GCGGTTGCGGCCGGGGAAGTGCCGTTTTCACTTGGATCTGACACGGGCGGATCGATTCGCCAGCCGGCATCTTTCTGCGG
CGTCGTCGGCTTAAAGCCTACATACGGACGCGTTTCCAGATACGGCCTTGTCGCATTTGCATCTTCATTGGATCAAATCG
GCCCGATCACAAGATCTGTCGAAGACAACGCCTACCTTCTGCAGGCGATTTCAGGCGTTGACAAAATGGATTCAACGAGC
GCCAATGTGGACGTGCCTGACTACCTGTCTGCGCTGACAGGCGATATTAAAGGCTTAAAAATCGCCGTTCCGAAAGAATA
TCTCGGCGAAGGGGTAAGCGAAGAAGCGAAGCAGTCCGTGCTTGAAGCGCTGAAAGTGCTCGAAAGCCTTGGCGCGACAT
GGGAAGAGGTATCGCTTCCGCACAGCAAATACGCGCTGGCGACTTACTACCTGCTTTCTTCATCTGAAGCGTCGGCAAAC
TTGGCGCGCTTCGACGGCATCCGCTACGGCTACCGCACGGACAATGCCGACAACCTGATCGACCTGTACAAACAGACGCG
TTCCGAAGGATTCGGAAACGAAGTCAAACGCCGCATCATGCTCGGTACGTTTGCTCTGAGCTCCGGCTATTACGACGCAT
ACTATAAAAAAGCGCAGAAAGTCAGAACGCTGATCAAAAAGGACTTTGAAGACGTATTTGCAAACTATGACGTCATCATC
GGACCGACAACGCCGACGCCGGCATTTAAAATCGGCGAAAAAACGAGCGATCCGCTGACCATGTACGCCAACGACATCCT
GACGATTCCTGTCAACCTTGCGGGCGTTCCGGGAATCAGCGTACCGTGCGGATTTGCGAACGGCTTGCCGCTCGGCCTGC
AAATCATCGGCAAACACTTTGACGAAAGCACAGTATACCGCGTCGCCCACGCATTCGAACAGGCGACAGACCACCATAAA
GCAAAACCTGAACTGTAA

Upstream 100 bases:

>100_bases
GCTTCCGGTTGAAGACGTCGTCAAAAATGCGCCTGATCATAAAGACGGCTACATCCGCGTGCCGTCAATCTTGGACTAAT
AAAGGAGGGAGCGAGAAAAC

Downstream 100 bases:

>100_bases
GGGGTGAAAAAACATGAACTTTGAAACGGTAATCGGACTTGAAGTCCACGTGGAGCTCAAAACAAAATCGAAAATTTTCT
CCAGTTCTCCAACGCCATTC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDEALDTRDEHGLLFGMPIGIKD
NIVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIGKLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAA
AVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTS
ANVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLPHSKYALATYYLLSSSEASAN
LARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVII
GPTTPTPAFKIGEKTSDPLTMYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHK
AKPEL

Sequences:

>Translated_485_residues
MSLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDEALDTRDEHGLLFGMPIGIKD
NIVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIGKLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAA
AVAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTS
ANVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLPHSKYALATYYLLSSSEASAN
LARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVII
GPTTPTPAFKIGEKTSDPLTMYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHK
AKPEL
>Mature_484_residues
SLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDEALDTRDEHGLLFGMPIGIKDN
IVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIGKLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAAA
VAAGEVPFSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTSA
NVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLPHSKYALATYYLLSSSEASANL
ARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVIIG
PTTPTPAFKIGEKTSDPLTMYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKA
KPEL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=37.3493975903614, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=26.5432098765432, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI166795287, Length=173, Percent_Identity=35.2601156069364, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=409, Percent_Identity=38.8753056234719, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=487, Percent_Identity=26.0780287474333, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=228, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=219, Percent_Identity=31.9634703196347, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=206, Percent_Identity=34.4660194174757, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=206, Percent_Identity=34.4660194174757, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=395, Percent_Identity=25.5696202531646, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=294, Percent_Identity=25.8503401360544, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI32565337, Length=92, Percent_Identity=40.2173913043478, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=39.0887290167866, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=30.7506053268765, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=463, Percent_Identity=24.4060475161987, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=40.9691629955947, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24652985, Length=500, Percent_Identity=26.2, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=500, Percent_Identity=26.2, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=500, Percent_Identity=26.2, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=500, Percent_Identity=26.2, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=26.5873015873016, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=27.5929549902153, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550774, Length=485, Percent_Identity=24.7422680412371, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648435, Length=485, Percent_Identity=24.7422680412371, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648437, Length=485, Percent_Identity=24.7422680412371, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=24.6938775510204, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=24.824355971897, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=24.824355971897, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52882; Mature: 52751

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDE
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALDTRDEHGLLFGMPIGIKDNIVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIG
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEE
KLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR
CCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
QPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCCCCC
ANVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLP
CCCCCHHHHHHHHCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCC
HSKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIM
CHHHHHHHHHEEECCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVIIGPTTPTPAFKIGEKTSDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCEE
MYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHK
EEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
AKPEL
CCCCC
>Mature Secondary Structure 
SLFDHKISELKRLIHNKEISISDLVDESYKRIHEVDGKVQAFLQLDEEKARAYAKELDE
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ALDTRDEHGLLFGMPIGIKDNIVTKDLRTTCASKILENFDPIYDATVVERLHEAEAVTIG
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEE
KLNMDEFAMGSSTENSGFKKTKNPWNLETVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR
CCCHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
QPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRSVEDNAYLLQAISGVDKMDSTS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCCCCC
ANVDVPDYLSALTGDIKGLKIAVPKEYLGEGVSEEAKQSVLEALKVLESLGATWEEVSLP
CCCCCHHHHHHHHCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCC
HSKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRSEGFGNEVKRRIM
CHHHHHHHHHEEECCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFANYDVIIGPTTPTPAFKIGEKTSDPLT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCCCEE
MYANDILTIPVNLAGVPGISVPCGFANGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHK
EEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
AKPEL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA