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The map label for this gene is nagB [H]

Identifier: 52784526

GI number: 52784526

Start: 731380

End: 732108

Strand: Direct

Name: nagB [H]

Synonym: BLi00726

Alternate gene names: 52784526

Gene position: 731380-732108 (Clockwise)

Preceding gene: 52784525

Following gene: 52784529

Centisome position: 17.32

GC content: 38.55

Gene sequence:

>729_bases
GTGAAAATTATTCATGTATCAGGTTATGAAGAAATGAGTGGAAAATCGGCTGATCTAATGGCTGAGCTCATTCAAAATTC
AAATAATCCTGTTATCGGCTTTGCAACAGGTTCGACACCGATTGGTTTATATAAATGCCTGATAAAAAAGTATCGGCAAA
AGGAAATTTCATTTAGGAATGTTACTGCTTTTAATTTAGACGAGTACGTTGGATTGGCTAAAGAAAATAAAAACAGCTAC
CATTATTATATGAATGAAAAATTATTCAAACATCTGGATATTAGTCGAAGGAATATCAATATCCCCAATGGGATGGCGGA
AGATTTGGAACAAGAATGCCTCAGCTACGACAGACTTGTTTCTGAAAAAGCAATTGACATACAAATATTAGGAATGGGAT
TGAATGGTCATATCGGTTTTAATGAACCGGGGACAAGCTTTAAAAGCAGAACCCATATCGTTGATTTGGATCAATCAACC
CGGCAGGCAAATTCCCGGTTCTTTCGTTCCTTGGACGAAGTACCGACAAAGGCATTAACAATGGGCATTGAAACAATAAT
GGAAAGTAAGCGGATCCTTCTGCTCGTGTCTGGAAATGAAAAATCTGAAGCCTTGGCACGGCTGCTGAACAGCACCCGTG
TCTCCCAAGAGTTCCCTGCTTCAATTTTAAGGAAACATAAAGATGTGACGGTGATGGCAGATGACGCAGCCTTAAATAAA
ATTCATTAA

Upstream 100 bases:

>100_bases
CTTACTGAAAAAGGTGTGGCGAAAATGCTTGGCCTTTTTGCAGTGCAGTTTAATACAGTAGTCAGTCTTGGACATTTTAT
TTTGTGGAAAGGAGAAAAAC

Downstream 100 bases:

>100_bases
ATTGAATGACTGACAAAATCGGTTAATTACATTGAAAATTTACACAAGGCATTGCTTTGAGGTATCTTCTAGCCATCAAT
CAGCCAAAAAACGACTGAAT

Product: NagB

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKIIHVSGYEEMSGKSADLMAELIQNSNNPVIGFATGSTPIGLYKCLIKKYRQKEISFRNVTAFNLDEYVGLAKENKNSY
HYYMNEKLFKHLDISRRNINIPNGMAEDLEQECLSYDRLVSEKAIDIQILGMGLNGHIGFNEPGTSFKSRTHIVDLDQST
RQANSRFFRSLDEVPTKALTMGIETIMESKRILLLVSGNEKSEALARLLNSTRVSQEFPASILRKHKDVTVMADDAALNK
IH

Sequences:

>Translated_242_residues
MKIIHVSGYEEMSGKSADLMAELIQNSNNPVIGFATGSTPIGLYKCLIKKYRQKEISFRNVTAFNLDEYVGLAKENKNSY
HYYMNEKLFKHLDISRRNINIPNGMAEDLEQECLSYDRLVSEKAIDIQILGMGLNGHIGFNEPGTSFKSRTHIVDLDQST
RQANSRFFRSLDEVPTKALTMGIETIMESKRILLLVSGNEKSEALARLLNSTRVSQEFPASILRKHKDVTVMADDAALNK
IH
>Mature_242_residues
MKIIHVSGYEEMSGKSADLMAELIQNSNNPVIGFATGSTPIGLYKCLIKKYRQKEISFRNVTAFNLDEYVGLAKENKNSY
HYYMNEKLFKHLDISRRNINIPNGMAEDLEQECLSYDRLVSEKAIDIQILGMGLNGHIGFNEPGTSFKSRTHIVDLDQST
RQANSRFFRSLDEVPTKALTMGIETIMESKRILLLVSGNEKSEALARLLNSTRVSQEFPASILRKHKDVTVMADDAALNK
IH

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion [H]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI13027378, Length=246, Percent_Identity=38.2113821138211, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI19923881, Length=210, Percent_Identity=40, Blast_Score=164, Evalue=5e-41,
Organism=Escherichia coli, GI1786893, Length=245, Percent_Identity=36.3265306122449, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789530, Length=230, Percent_Identity=30, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994958, Length=243, Percent_Identity=27.5720164609054, Blast_Score=81, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554876, Length=238, Percent_Identity=40.3361344537815, Blast_Score=183, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24581960, Length=209, Percent_Identity=41.6267942583732, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19920764, Length=209, Percent_Identity=41.6267942583732, Blast_Score=181, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: =3.5.99.6 [H]

Molecular weight: Translated: 27333; Mature: 27333

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIHVSGYEEMSGKSADLMAELIQNSNNPVIGFATGSTPIGLYKCLIKKYRQKEISFRN
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
VTAFNLDEYVGLAKENKNSYHYYMNEKLFKHLDISRRNINIPNGMAEDLEQECLSYDRLV
EEEECHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SEKAIDIQILGMGLNGHIGFNEPGTSFKSRTHIVDLDQSTRQANSRFFRSLDEVPTKALT
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHH
MGIETIMESKRILLLVSGNEKSEALARLLNSTRVSQEFPASILRKHKDVTVMADDAALNK
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHCC
IH
CC
>Mature Secondary Structure
MKIIHVSGYEEMSGKSADLMAELIQNSNNPVIGFATGSTPIGLYKCLIKKYRQKEISFRN
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
VTAFNLDEYVGLAKENKNSYHYYMNEKLFKHLDISRRNINIPNGMAEDLEQECLSYDRLV
EEEECHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SEKAIDIQILGMGLNGHIGFNEPGTSFKSRTHIVDLDQSTRQANSRFFRSLDEVPTKALT
HCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHH
MGIETIMESKRILLLVSGNEKSEALARLLNSTRVSQEFPASILRKHKDVTVMADDAALNK
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCHHCC
IH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]