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The map label for this gene is purN [H]

Identifier: 52784503

GI number: 52784503

Start: 706498

End: 707085

Strand: Direct

Name: purN [H]

Synonym: BLi00702

Alternate gene names: 52784503

Gene position: 706498-707085 (Clockwise)

Preceding gene: 52784502

Following gene: 52784504

Centisome position: 16.73

GC content: 50.0

Gene sequence:

>588_bases
ATGAAAAAATTCGCGGTATTTGCATCAGGAAGCGGAACGAATTTTGAAGCAATCGAAAGAAGAATGAGAGAAGAGAACTG
GGATGCCGAAATTGTCCTCGTCGTCTGTGACAAGCCCGATGCGAAAGTGCTTGAAAGGGCGGAAAAAGCCGGAATCCCGT
CGTTCGCCTTCCAGCCGAAATCGTTCGACAATAAAGCTGCATTTGAACAAGTGATCGTTGAACAGCTCCGCCTTCACGGA
GCGGAATGGATTGTGCTGGCGGGGTATATGAGGCTGATCGGCGATACGCTTCTTTCGGCTTACCGGAATAAAATCATCAA
CATCCATCCGTCGCTCCTGCCGGCGTTTCCCGGCATCGACGCGGTCGGACAAGCGTACAGAGCAGGCGTGAAAGTGGCAG
GCATCACGGTTCACTACGTTGACGAAGGCATGGACACCGGTCCGATTATCGCCCAGCGCGCCGTTGAGCTTGAAAAAAGC
GAAACACTCGCGTCTCTTGAAGAAAAGATACACAAATTGGAGCATGAACTTTATCCCGAAGTAATCAAAGAGCTTCTTGA
ACTAAATGGCAGAGGTGAAAAGGAATGA

Upstream 100 bases:

>100_bases
CAGACGTGATCCAAACGCTCGAAAACAACGGTGAAAAAGCCTATCTCATCGGCAGAGTGAAAGCCGGAAGCGGCGTTGTG
TTTGGCGGTGCCGGTCTTTC

Downstream 100 bases:

>100_bases
CGATCAAACGCGCATTAATCAGCGTATCTGATAAAACGAACATTGTCCCTTTTGTAAAAGAATTGACAGAGCTTGGCGTT
GAAGTGATTTCAACAGGGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKKFAVFASGSGTNFEAIERRMREENWDAEIVLVVCDKPDAKVLERAEKAGIPSFAFQPKSFDNKAAFEQVIVEQLRLHG
AEWIVLAGYMRLIGDTLLSAYRNKIINIHPSLLPAFPGIDAVGQAYRAGVKVAGITVHYVDEGMDTGPIIAQRAVELEKS
ETLASLEEKIHKLEHELYPEVIKELLELNGRGEKE

Sequences:

>Translated_195_residues
MKKFAVFASGSGTNFEAIERRMREENWDAEIVLVVCDKPDAKVLERAEKAGIPSFAFQPKSFDNKAAFEQVIVEQLRLHG
AEWIVLAGYMRLIGDTLLSAYRNKIINIHPSLLPAFPGIDAVGQAYRAGVKVAGITVHYVDEGMDTGPIIAQRAVELEKS
ETLASLEEKIHKLEHELYPEVIKELLELNGRGEKE
>Mature_195_residues
MKKFAVFASGSGTNFEAIERRMREENWDAEIVLVVCDKPDAKVLERAEKAGIPSFAFQPKSFDNKAAFEQVIVEQLRLHG
AEWIVLAGYMRLIGDTLLSAYRNKIINIHPSLLPAFPGIDAVGQAYRAGVKVAGITVHYVDEGMDTGPIIAQRAVELEKS
ETLASLEEKIHKLEHELYPEVIKELLELNGRGEKE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=181, Percent_Identity=37.5690607734807, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869995, Length=181, Percent_Identity=37.5690607734807, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI209869993, Length=181, Percent_Identity=37.5690607734807, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI238814322, Length=141, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI21614513, Length=169, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788846, Length=178, Percent_Identity=32.5842696629214, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787483, Length=136, Percent_Identity=34.5588235294118, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=37.7777777777778, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=29.7979797979798, Blast_Score=71, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=38.2978723404255, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21761; Mature: 21761

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFAVFASGSGTNFEAIERRMREENWDAEIVLVVCDKPDAKVLERAEKAGIPSFAFQPK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
SFDNKAAFEQVIVEQLRLHGAEWIVLAGYMRLIGDTLLSAYRNKIINIHPSLLPAFPGID
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHCCCCCHH
AVGQAYRAGVKVAGITVHYVDEGMDTGPIIAQRAVELEKSETLASLEEKIHKLEHELYPE
HHHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIKELLELNGRGEKE
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKFAVFASGSGTNFEAIERRMREENWDAEIVLVVCDKPDAKVLERAEKAGIPSFAFQPK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCC
SFDNKAAFEQVIVEQLRLHGAEWIVLAGYMRLIGDTLLSAYRNKIINIHPSLLPAFPGID
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHCCCCCHH
AVGQAYRAGVKVAGITVHYVDEGMDTGPIIAQRAVELEKSETLASLEEKIHKLEHELYPE
HHHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIKELLELNGRGEKE
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]