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The map label for this gene is pbuG [H]

Identifier: 52784489

GI number: 52784489

Start: 693215

End: 694537

Strand: Direct

Name: pbuG [H]

Synonym: BLi00688

Alternate gene names: 52784489

Gene position: 693215-694537 (Clockwise)

Preceding gene: 52784488

Following gene: 52784490

Centisome position: 16.42

GC content: 48.22

Gene sequence:

>1323_bases
TTGAAGCGTTACTTTCAGTTTGAAGAATTGGGCACAAATTATCGGAGAGAAATGATCGGCGGACTCACCACATTTTTGTC
CATGGCATATATTTTGATCGTCAACCCGCTTACCCTTTCTCTGGCGTCGGTGGAAAATTTCCCGGATGCATTGAGAATGG
ATCAAAGCGCGGTCTTTACGGCAACCGCGGTTGCAGCAGCCGTCGGTTGTTTTATCATGGGGCTGCTCGCGAAGTATCCG
ATCGCTCTGGCGCCCGGAATGGGGCTTAACGCATTCTTTGCATTCTCAGTCATCCTGAATATGCAGATTCCTTGGCAGAC
GGCTCTTTCAGGCGTTATGGTTTCCGGACTTATTTTTATCGTTCTTTCGCTATCCGGTTTAAGGGAAAAAATTATCAATT
CGATTCCTTATGAGCTGAAAATGGCTGTCGGTGCGGGGATTGGCCTGTTTATCGCTTTCGTCGGTTTGCAGGGCTCCAAA
ATCGTCGTATCCAACCCGGATACGATTGTGTCGTTTGGGAATATTCATTCAGGTCCCGTTCTTTTGACTGTTTTCGGACT
GATCATCACTGTCATTTTAATGGTATTGCGCGTCAACGGCGGCATTTTCTTCGGGATGCTGATCACGGCGATTGTCGGCA
TGATTTTCGGACAGATTGACGTTCCTGACAAAATCGTCGGTGCCGTTCCTAGTCTTGCGCCAACATTTGGACAAGCCTTT
ATCCACCTTCATGATATCTTTACGGTGCAAATGCTTGTCGTCATTTTAACGTTTTTATTCGTCGACTTTTTTGATACGGC
CGGTACTTTGGTGGGTGTTGCATCACAAGCCGGTTTGATGAAGGATAACAAACTGCCGCGCGCGGGAAGAGCTTTGCTTG
CCGATTCTTCCGCAACTGTGGCCGGAGCGATCCTTGGTACATCGACAACGACCGCTTATGTTGAGTCAAGCGCCGGGGTA
GCGGCCGGAGCGAGAAGCGGTTTTGCGGCGATTGTAACCGGCGTTTTGTTCTTGCTGTCGCTGTTCTTCTCGCCCGTACT
CAGCGTTATCACTTCACAAGTGACGGCGCCTGCTTTAATCATCGTCGGCGTGCTGATGGTGGCGAATCTCAACAAAATCG
ACTGGACCAAATTTGAGATTGCGGTGCCCGCATTCTTCACGATGATCGCGATGCCGCTGACATATAGCATCGCCACCGGA
ATCGCGATCGGATTTATTTTCTACCCGATCACGATGATTTTTAAAGGCAAAGCAAAAGAGATCCATCCGATTATGTACGG
ACTGGCTGTCGTATTTATTCTTTATTTCATTTTCTTGAAATAG

Upstream 100 bases:

>100_bases
TGCCGTAGTCAAGCGTCCCGCCAGTTGGGGCGTTTTTTGTTTAAAAGCAAAGGTTTATGAAACAATAAAAGCGACAGACA
AACGAGAGGAGTATACCATT

Downstream 100 bases:

>100_bases
GCTGTAGGAAAAAGCGCTTGTTCATTTGAACGAGCGCCTTTTTTTATTTCCAATTCATCAAAATCGGAACCTTCCGCCTT
CGTTTTTCGTCATATATAAG

Product: PbuG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKRYFQFEELGTNYRREMIGGLTTFLSMAYILIVNPLTLSLASVENFPDALRMDQSAVFTATAVAAAVGCFIMGLLAKYP
IALAPGMGLNAFFAFSVILNMQIPWQTALSGVMVSGLIFIVLSLSGLREKIINSIPYELKMAVGAGIGLFIAFVGLQGSK
IVVSNPDTIVSFGNIHSGPVLLTVFGLIITVILMVLRVNGGIFFGMLITAIVGMIFGQIDVPDKIVGAVPSLAPTFGQAF
IHLHDIFTVQMLVVILTFLFVDFFDTAGTLVGVASQAGLMKDNKLPRAGRALLADSSATVAGAILGTSTTTAYVESSAGV
AAGARSGFAAIVTGVLFLLSLFFSPVLSVITSQVTAPALIIVGVLMVANLNKIDWTKFEIAVPAFFTMIAMPLTYSIATG
IAIGFIFYPITMIFKGKAKEIHPIMYGLAVVFILYFIFLK

Sequences:

>Translated_440_residues
MKRYFQFEELGTNYRREMIGGLTTFLSMAYILIVNPLTLSLASVENFPDALRMDQSAVFTATAVAAAVGCFIMGLLAKYP
IALAPGMGLNAFFAFSVILNMQIPWQTALSGVMVSGLIFIVLSLSGLREKIINSIPYELKMAVGAGIGLFIAFVGLQGSK
IVVSNPDTIVSFGNIHSGPVLLTVFGLIITVILMVLRVNGGIFFGMLITAIVGMIFGQIDVPDKIVGAVPSLAPTFGQAF
IHLHDIFTVQMLVVILTFLFVDFFDTAGTLVGVASQAGLMKDNKLPRAGRALLADSSATVAGAILGTSTTTAYVESSAGV
AAGARSGFAAIVTGVLFLLSLFFSPVLSVITSQVTAPALIIVGVLMVANLNKIDWTKFEIAVPAFFTMIAMPLTYSIATG
IAIGFIFYPITMIFKGKAKEIHPIMYGLAVVFILYFIFLK
>Mature_440_residues
MKRYFQFEELGTNYRREMIGGLTTFLSMAYILIVNPLTLSLASVENFPDALRMDQSAVFTATAVAAAVGCFIMGLLAKYP
IALAPGMGLNAFFAFSVILNMQIPWQTALSGVMVSGLIFIVLSLSGLREKIINSIPYELKMAVGAGIGLFIAFVGLQGSK
IVVSNPDTIVSFGNIHSGPVLLTVFGLIITVILMVLRVNGGIFFGMLITAIVGMIFGQIDVPDKIVGAVPSLAPTFGQAF
IHLHDIFTVQMLVVILTFLFVDFFDTAGTLVGVASQAGLMKDNKLPRAGRALLADSSATVAGAILGTSTTTAYVESSAGV
AAGARSGFAAIVTGVLFLLSLFFSPVLSVITSQVTAPALIIVGVLMVANLNKIDWTKFEIAVPAFFTMIAMPLTYSIATG
IAIGFIFYPITMIFKGKAKEIHPIMYGLAVVFILYFIFLK

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=441, Percent_Identity=38.5487528344671, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790150, Length=441, Percent_Identity=40.1360544217687, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1790499, Length=428, Percent_Identity=37.1495327102804, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI48994909, Length=430, Percent_Identity=35.8139534883721, Blast_Score=222, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 46856; Mature: 46856

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]