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The map label for this gene is ydaP [H]

Identifier: 52784340

GI number: 52784340

Start: 527329

End: 529050

Strand: Direct

Name: ydaP [H]

Synonym: BLi00520

Alternate gene names: 52784340

Gene position: 527329-529050 (Clockwise)

Preceding gene: 52784339

Following gene: 52784341

Centisome position: 12.49

GC content: 49.36

Gene sequence:

>1722_bases
ATGGCAAACAAAACCGCAGGAAAAATAGCTGCAGAACTGCTGAAGGAATGGAACATTGATCATGTTTACGGCATGCCGGG
CGACAGCGTCAACGAATTTATCGATGAATTGCGGCATGAAGAAAACAGCCTCCGCTTCATTCAAGTGCGTCATGAAGAAA
CCGCTGCTCTCGCTGCCGCAGCCGATGCAAAGCTGACAGGCAAAATCGGCGTCTGCCTTTCGATAGCGGGCCCGGGCGCC
GTTCATCTGCTGAACGGCTTGTATGATGCGAAAGCTGACGGGGCGCCTGTGCTTGCGATTACAGGACAGGTGTCTTCGGA
TGAAATCGGGAGAGATTATTTTCAGGAGATCGGTCTTGAACGAATGTTTGAGGATGTGGCTCTCTTTAATCAGCAGGTGC
ATTCGGCTGAAGCCCTGCCAGATCTTTTGAATCAGGCGATCCGTACCGCATACAGCCAAAAAGGGGCCGCCGTTCTGAGC
GTATCCGATGATCTGTTTGCAGAAAAGATTAAGCGGAAGCCGGTTTATACGTCTGCGCTTTATATCGAAGGCGATCTTGA
ACCGAAGAAAAGCCAGCTGATGCAGTGTGCACAATTGATTAACCAGGCAAAAAAACCGGTGATTTTAGCGGGACGGGGAA
TGAAATCGGCGAGAGACGAGCTGCTCGAGTTTGCAGATAAAGCGGCCGCTCCGATCATTGTCACACTCCCAGCGAAAGGG
GTCGTACCTGATCGGCACCCGCATATGCTCGGGAACCTCGGACACATCGGCACAAAGCCTGCATATGAAGCGATGGAGGA
AAGCGATCTGTTGATCATGCTGGGGACTTCATTTCCGTACCGCGATTATCTGCCGGAAGATGCCCCGGCTATTCAGCTTG
ACAACAATCCGGCGAAAATCGGCAAACGGTATCCTGTCACCGCCGGCCTTGTCTGCGATGCGAAAAAAGGGCTTTTTGAA
CTGACGAAAACAATTGAACGGAAATCGAACCGCGCATTTTTGGAAAGCTGTATACAACATATGAGGAAGTGGCGTTATGA
AGTTGAAAAAGACGAACAGGTAGCGACAGAGCCGCTGAAGCCTCAGCAGGTGATTGCAAGACTTGAGGACGCGGTCGCAG
ATGATGCGGTTCTGTCCGTTGACGTCGGAAATGTGACGGTTTGGACGGCGCGCCATTTTAATATGACCAATCAGGATTTT
TTGATATCAAGCTGGCTTGGCACGATGGGATGCGGTCTTCCAGGCGCGATTTCTGCAAAGCTTTCCCATCCGGAACGCCA
GGTTGTAGCCGTGTGCGGAGACGGCGGTTTCAGCATGTCTATGCACGATTTTCCGACTGCTGTCAAATACGAACTGCCCA
TTGTTGTTGTGATTTTAAATAATCAGAACTTGGGAATGATTCAATATGAACAGCAGGAAAAAGGGCATGTTAATTATGCG
ACGGCGCTCGAAAACGTCGATTATGCCAAGTTTGCCGAAGCCTGCGGCGGGAAGGGCTTCAGCGTGACAAAGCACGAAGA
GCTTATTCCTGCTTTGAAAAGCGCCTTTCATTCACAAAAGCCGTCGATTATCGATGTGGCAATTGAAGACGAACCGCCTC
TTCCGGGAAAAATATCCTATACACAAGCTGTAAACTACAGCAAATATATGATCAAAAAACTAGTTGAGAAAAAAGAGCTC
GACTTGCCGCCGCTGAAAAAAAGTTTAAAAAGGATTTTTTAG

Upstream 100 bases:

>100_bases
GCAGAGCCATGCCTCTGCAGGCTGTTTTTTTGGCGGGTACGCTGCAAAACGTTTCAGCAGAAATGGAAGGAGGTATGGAA
AAAACAAAGGAGGACGTGAT

Downstream 100 bases:

>100_bases
TATGATGCCAAGGGAGATCGTCCCTTGGCTTTATAACGGTCAGATGTTAAAATTGAATGGGTAATATTGGAATGGCCGCG
TATGTTTTCTTTTTGCATCA

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MANKTAGKIAAELLKEWNIDHVYGMPGDSVNEFIDELRHEENSLRFIQVRHEETAALAAAADAKLTGKIGVCLSIAGPGA
VHLLNGLYDAKADGAPVLAITGQVSSDEIGRDYFQEIGLERMFEDVALFNQQVHSAEALPDLLNQAIRTAYSQKGAAVLS
VSDDLFAEKIKRKPVYTSALYIEGDLEPKKSQLMQCAQLINQAKKPVILAGRGMKSARDELLEFADKAAAPIIVTLPAKG
VVPDRHPHMLGNLGHIGTKPAYEAMEESDLLIMLGTSFPYRDYLPEDAPAIQLDNNPAKIGKRYPVTAGLVCDAKKGLFE
LTKTIERKSNRAFLESCIQHMRKWRYEVEKDEQVATEPLKPQQVIARLEDAVADDAVLSVDVGNVTVWTARHFNMTNQDF
LISSWLGTMGCGLPGAISAKLSHPERQVVAVCGDGGFSMSMHDFPTAVKYELPIVVVILNNQNLGMIQYEQQEKGHVNYA
TALENVDYAKFAEACGGKGFSVTKHEELIPALKSAFHSQKPSIIDVAIEDEPPLPGKISYTQAVNYSKYMIKKLVEKKEL
DLPPLKKSLKRIF

Sequences:

>Translated_573_residues
MANKTAGKIAAELLKEWNIDHVYGMPGDSVNEFIDELRHEENSLRFIQVRHEETAALAAAADAKLTGKIGVCLSIAGPGA
VHLLNGLYDAKADGAPVLAITGQVSSDEIGRDYFQEIGLERMFEDVALFNQQVHSAEALPDLLNQAIRTAYSQKGAAVLS
VSDDLFAEKIKRKPVYTSALYIEGDLEPKKSQLMQCAQLINQAKKPVILAGRGMKSARDELLEFADKAAAPIIVTLPAKG
VVPDRHPHMLGNLGHIGTKPAYEAMEESDLLIMLGTSFPYRDYLPEDAPAIQLDNNPAKIGKRYPVTAGLVCDAKKGLFE
LTKTIERKSNRAFLESCIQHMRKWRYEVEKDEQVATEPLKPQQVIARLEDAVADDAVLSVDVGNVTVWTARHFNMTNQDF
LISSWLGTMGCGLPGAISAKLSHPERQVVAVCGDGGFSMSMHDFPTAVKYELPIVVVILNNQNLGMIQYEQQEKGHVNYA
TALENVDYAKFAEACGGKGFSVTKHEELIPALKSAFHSQKPSIIDVAIEDEPPLPGKISYTQAVNYSKYMIKKLVEKKEL
DLPPLKKSLKRIF
>Mature_572_residues
ANKTAGKIAAELLKEWNIDHVYGMPGDSVNEFIDELRHEENSLRFIQVRHEETAALAAAADAKLTGKIGVCLSIAGPGAV
HLLNGLYDAKADGAPVLAITGQVSSDEIGRDYFQEIGLERMFEDVALFNQQVHSAEALPDLLNQAIRTAYSQKGAAVLSV
SDDLFAEKIKRKPVYTSALYIEGDLEPKKSQLMQCAQLINQAKKPVILAGRGMKSARDELLEFADKAAAPIIVTLPAKGV
VPDRHPHMLGNLGHIGTKPAYEAMEESDLLIMLGTSFPYRDYLPEDAPAIQLDNNPAKIGKRYPVTAGLVCDAKKGLFEL
TKTIERKSNRAFLESCIQHMRKWRYEVEKDEQVATEPLKPQQVIARLEDAVADDAVLSVDVGNVTVWTARHFNMTNQDFL
ISSWLGTMGCGLPGAISAKLSHPERQVVAVCGDGGFSMSMHDFPTAVKYELPIVVVILNNQNLGMIQYEQQEKGHVNYAT
ALENVDYAKFAEACGGKGFSVTKHEELIPALKSAFHSQKPSIIDVAIEDEPPLPGKISYTQAVNYSKYMIKKLVEKKELD
LPPLKKSLKRIF

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=25.2631578947368, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21361361, Length=558, Percent_Identity=22.9390681003584, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=34.9122807017544, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1790104, Length=540, Percent_Identity=29.6296296296296, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=28.9855072463768, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=26.9026548672566, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788716, Length=548, Percent_Identity=26.6423357664234, Blast_Score=148, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=24.3727598566308, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=24.3727598566308, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=21.326164874552, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=30.8510638297872, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=25.8064516129032, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321524, Length=502, Percent_Identity=22.7091633466135, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=22.6452905811623, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=502, Percent_Identity=21.1155378486056, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922626, Length=558, Percent_Identity=26.1648745519713, Blast_Score=168, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 63039; Mature: 62908

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTAGKIAAELLKEWNIDHVYGMPGDSVNEFIDELRHEENSLRFIQVRHEETAALAAA
CCCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHH
ADAKLTGKIGVCLSIAGPGAVHLLNGLYDAKADGAPVLAITGQVSSDEIGRDYFQEIGLE
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCHH
RMFEDVALFNQQVHSAEALPDLLNQAIRTAYSQKGAAVLSVSDDLFAEKIKRKPVYTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEEE
YIEGDLEPKKSQLMQCAQLINQAKKPVILAGRGMKSARDELLEFADKAAAPIIVTLPAKG
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCC
VVPDRHPHMLGNLGHIGTKPAYEAMEESDLLIMLGTSFPYRDYLPEDAPAIQLDNNPAKI
CCCCCCCHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHCCCCCCCEEEECCCHHHC
GKRYPVTAGLVCDAKKGLFELTKTIERKSNRAFLESCIQHMRKWRYEVEKDEQVATEPLK
CCCCCCEECEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
PQQVIARLEDAVADDAVLSVDVGNVTVWTARHFNMTNQDFLISSWLGTMGCGLPGAISAK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
LSHPERQVVAVCGDGGFSMSMHDFPTAVKYELPIVVVILNNQNLGMIQYEQQEKGHVNYA
CCCCCEEEEEEECCCCCCCCHHCCCCEEEEECCEEEEEECCCCCCEEEECCCCCCCCCHH
TALENVDYAKFAEACGGKGFSVTKHEELIPALKSAFHSQKPSIIDVAIEDEPPLPGKISY
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEH
TQAVNYSKYMIKKLVEKKELDLPPLKKSLKRIF
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ANKTAGKIAAELLKEWNIDHVYGMPGDSVNEFIDELRHEENSLRFIQVRHEETAALAAA
CCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHH
ADAKLTGKIGVCLSIAGPGAVHLLNGLYDAKADGAPVLAITGQVSSDEIGRDYFQEIGLE
CCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCHH
RMFEDVALFNQQVHSAEALPDLLNQAIRTAYSQKGAAVLSVSDDLFAEKIKRKPVYTSAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEEE
YIEGDLEPKKSQLMQCAQLINQAKKPVILAGRGMKSARDELLEFADKAAAPIIVTLPAKG
EEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCC
VVPDRHPHMLGNLGHIGTKPAYEAMEESDLLIMLGTSFPYRDYLPEDAPAIQLDNNPAKI
CCCCCCCHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHCCCCCCCEEEECCCHHHC
GKRYPVTAGLVCDAKKGLFELTKTIERKSNRAFLESCIQHMRKWRYEVEKDEQVATEPLK
CCCCCCEECEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCC
PQQVIARLEDAVADDAVLSVDVGNVTVWTARHFNMTNQDFLISSWLGTMGCGLPGAISAK
HHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCC
LSHPERQVVAVCGDGGFSMSMHDFPTAVKYELPIVVVILNNQNLGMIQYEQQEKGHVNYA
CCCCCEEEEEEECCCCCCCCHHCCCCEEEEECCEEEEEECCCCCCEEEECCCCCCCCCHH
TALENVDYAKFAEACGGKGFSVTKHEELIPALKSAFHSQKPSIIDVAIEDEPPLPGKISY
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEH
TQAVNYSKYMIKKLVEKKELDLPPLKKSLKRIF
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]