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The map label for this gene is kipI [H]

Identifier: 52784321

GI number: 52784321

Start: 507391

End: 508125

Strand: Direct

Name: kipI [H]

Synonym: BLi00501

Alternate gene names: 52784321

Gene position: 507391-508125 (Clockwise)

Preceding gene: 52784320

Following gene: 52784322

Centisome position: 12.02

GC content: 53.33

Gene sequence:

>735_bases
TTGAAAAATCTTTCGAATGCGACAATGTCTCCGCTCGGCGACTCCGCGGTTATCATCCAGCTTGGAGAGGGGATAAGCGA
ACAGACGCACCGTGTCGTCAGCGCGCTGACAAAGCGGATTGAAAACGATCCGTTTCCCGGTTTTATTGAGTGTGTGCCGG
CTTTCACGAGTGTGACCGTTTTCTATCAGCCATTTGAAGTATACCGCAAGATGGAGAGCGGCAAGGCTGTTGATTCTCCG
TATGAAAAGGTAAAAGCCCTCCTCGACCATCATCTCCAAGAGCTCACAATGGAAGAAGAGACAGATCAGCGCACGGTGGA
AATTCCGGTTTGTTACGGCGGCCGTTTCGGCCCTGATTTGGAGGAAGTGGCCGACATCAACGGCCTGACTGCACAGGAAG
TCATCGATATTCACACCTCGGGTGAGTACCTCGTTTATATGATCGGCTTTGCGCCGGGCTTCCCTTACCTAGGCGGAATG
TCTGAAAAGATAGCCGCACCGCGGCGGTCATCTCCTCGGACTTCGATTCCCGCCGGTTCAGTCGGAATAGCCGGCATGCA
AACAGGCGTCTATCCCCTTTCAACGCCGGGCGGCTGGCAGCTGATCGGCAATACGCCGCTTGAATTATTCAAGCCTTATG
AACAGCCTCCGAGCCTTCTGAGAGCAGGGGATATCGTGAAATTTGTCAGCGTGACAGAAGAGGAGTATCACGCGCTGAAG
GAGGGGAAATCTTGA

Upstream 100 bases:

>100_bases
ATGAACAATTTGGTGTGTTATAGGATTTCAAAATCAATACAGAGCGGTTTATCTGTATTGATTTTTAATTTAAGGGGATG
AAAGGCAAGGTGAAACAGAA

Downstream 100 bases:

>100_bases
GCATAGAAGTGTTAAAGCCGGGTCTGATGACAACGGTTCAAGACTTGGGCCGCACAGGTTTTCAAAAATACGGCGTGCTT
GTCAGCGGTGCCATGGATGC

Product: KipI

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKNLSNATMSPLGDSAVIIQLGEGISEQTHRVVSALTKRIENDPFPGFIECVPAFTSVTVFYQPFEVYRKMESGKAVDSP
YEKVKALLDHHLQELTMEEETDQRTVEIPVCYGGRFGPDLEEVADINGLTAQEVIDIHTSGEYLVYMIGFAPGFPYLGGM
SEKIAAPRRSSPRTSIPAGSVGIAGMQTGVYPLSTPGGWQLIGNTPLELFKPYEQPPSLLRAGDIVKFVSVTEEEYHALK
EGKS

Sequences:

>Translated_244_residues
MKNLSNATMSPLGDSAVIIQLGEGISEQTHRVVSALTKRIENDPFPGFIECVPAFTSVTVFYQPFEVYRKMESGKAVDSP
YEKVKALLDHHLQELTMEEETDQRTVEIPVCYGGRFGPDLEEVADINGLTAQEVIDIHTSGEYLVYMIGFAPGFPYLGGM
SEKIAAPRRSSPRTSIPAGSVGIAGMQTGVYPLSTPGGWQLIGNTPLELFKPYEQPPSLLRAGDIVKFVSVTEEEYHALK
EGKS
>Mature_244_residues
MKNLSNATMSPLGDSAVIIQLGEGISEQTHRVVSALTKRIENDPFPGFIECVPAFTSVTVFYQPFEVYRKMESGKAVDSP
YEKVKALLDHHLQELTMEEETDQRTVEIPVCYGGRFGPDLEEVADINGLTAQEVIDIHTSGEYLVYMIGFAPGFPYLGGM
SEKIAAPRRSSPRTSIPAGSVGIAGMQTGVYPLSTPGGWQLIGNTPLELFKPYEQPPSLLRAGDIVKFVSVTEEEYHALK
EGKS

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=222, Percent_Identity=41.4414414414414, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26702; Mature: 26702

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLSNATMSPLGDSAVIIQLGEGISEQTHRVVSALTKRIENDPFPGFIECVPAFTSVTV
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHE
FYQPFEVYRKMESGKAVDSPYEKVKALLDHHLQELTMEEETDQRTVEIPVCYGGRFGPDL
EECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCH
EEVADINGLTAQEVIDIHTSGEYLVYMIGFAPGFPYLGGMSEKIAAPRRSSPRTSIPAGS
HHHHHCCCCCHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
VGIAGMQTGVYPLSTPGGWQLIGNTPLELFKPYEQPPSLLRAGDIVKFVSVTEEEYHALK
CCCCHHCCCCEECCCCCCEEEECCCCHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGKS
CCCC
>Mature Secondary Structure
MKNLSNATMSPLGDSAVIIQLGEGISEQTHRVVSALTKRIENDPFPGFIECVPAFTSVTV
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHE
FYQPFEVYRKMESGKAVDSPYEKVKALLDHHLQELTMEEETDQRTVEIPVCYGGRFGPDL
EECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCH
EEVADINGLTAQEVIDIHTSGEYLVYMIGFAPGFPYLGGMSEKIAAPRRSSPRTSIPAGS
HHHHHCCCCCHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
VGIAGMQTGVYPLSTPGGWQLIGNTPLELFKPYEQPPSLLRAGDIVKFVSVTEEEYHALK
CCCCHHCCCCEECCCCCCEEEECCCCHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EGKS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]