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The map label for this gene is nasF [H]

Identifier: 52784306

GI number: 52784306

Start: 492658

End: 494118

Strand: Direct

Name: nasF [H]

Synonym: BLi00486

Alternate gene names: 52784306

Gene position: 492658-494118 (Clockwise)

Preceding gene: 52784305

Following gene: 52784308

Centisome position: 11.67

GC content: 52.36

Gene sequence:

>1461_bases
ATGGGGAATGGGAAAGTTTATTTTGTCGGGGCCGGACCGGGCGATTCAGGATTGATCACATTAAAAGGAAGAACGCTGAT
CGAGGCGGCGGATGTCATATTGTATGACAGGCTCGTCAATTCCCGGCTGCTTGAGCATGCAAAAGCATCATGCGAATTTG
TCTACTGCGGCAAGCTCCCGGACCGCCATTATATGAGGCAAAGTGAAATCAATGCACTGCTCATTGAAAAGGGGCTGAAA
GGCCTCAAAGTGGTCCGGCTGAAAGGAGGAGACCCGAGCGTGTTTGGCAGAGTCGGGGAAGAAGCGGCCGCCGTCAGCGA
ACACGGCATCCCGTACGAGATGGTGCCAGGCATTACATCGGGAATCGCCGCTCCTCTTTATGCCGGACTGCCTGTGACCC
ACCGCGATTTCGCTTCATCCTTTGCCGTTGTCACCGCCCATGATAAATCGGGCAAACCGGAGATGAATTGGGAAGGGCTG
GCCAAGGGCGTCCAAACCCTCGTCTTTTACATGGGGATCAAAAACCTTGGTTATATATGCGAGCAGCTGATGAAACACGG
CAAGTCCCCCCTTGTTCCCGCAATGGCCATCCAATGGGGCACATGGGGCCGGCAGCGGAGTGTTAAAGGGACATTGCAGA
ATATCAGGGAGAAAATAACCGAGCATCAGATCAAAAATCCCGCGATTATCGTCATCGGCGACATCGTATCATTCAGGCAG
AACAGCTGGTTTGAAGAAAAGCCTTTGATCGGGCTGAACATCATGGCAGTCAAAAGCTGTGAAGAAGAACAAATTCCAGT
GGAGGCGCTTCGTGAACAAGGCGCTGAAGTTGTGGAATGGCCGAAATGGAAATCGGCCGAAACGGTGCCTGACGATGCAC
TTTTAGGTCGAATCGCTTGTTTTGACAGCCTGCTGTTCACCTCAAGTGCGTCACTGCGCAGATTTTTCAGCCGGCTTGCT
GCTTTGAAGATCGATGTCAGGGAAATGCGCGGAAGGCTTTACGCTTCAGATCAGCATGTAAAAGCAGCTCTTGAAGAACG
GGGATTTGAAGCCTTTCTGCAAACGGAAATGCCGGTCTCGGGCAGCTGTTTGCTGATCGGCAGCCGGGAAGCCGTGCAAA
ATTACCCGCACTCCGGGTGCGAGGTATTGATTACACATGACAGGGTGATCGACAGCCGCTTTACCTCGATCATCAAACGG
GAAATTGATGAGTTTCCTATTGATATAGTGTTGTTTTCAAATCGAACATGCGTCGAGCTGTTCGTAAACGAGGCGGAGAC
AGTCGGCCTCCATCCGGAAAAAACGGCTGCCCGTCTTCAGGCGTTCTGCCTCACTGAAGAAGCGGGGGAGAGAGCTGCAG
CCGTAGGGTTTCAGCGCGTTCATGCAGTGAATGATCGCGATCGCCTGCCGGATGTCATCAAAGAGATCAACCGAACAGCC
GAATTTGTCTGTCAGAGCTGA

Upstream 100 bases:

>100_bases
ACGCAATATTTCAAAAGCCTTAAGGGTCGCAGAACGTGACCCCTCTTTATTAAAAAAACAAACGCGTTCACATAATCGGC
AGATGGAAGGATGACAGTTG

Downstream 100 bases:

>100_bases
AAAAAGCAGGGGGAACCCTGCTTTTTTAGCGTGCGGCACTGCTTCTGAACAACACCTCTTTTAAGTAGCAAAGGTCTGCC
TCTATATGCTTCCTCATTGA

Product: NasF

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNGKVYFVGAGPGDSGLITLKGRTLIEAADVILYDRLVNSRLLEHAKASCEFVYCGKLPDRHYMRQSEINALLIEKGLK
GLKVVRLKGGDPSVFGRVGEEAAAVSEHGIPYEMVPGITSGIAAPLYAGLPVTHRDFASSFAVVTAHDKSGKPEMNWEGL
AKGVQTLVFYMGIKNLGYICEQLMKHGKSPLVPAMAIQWGTWGRQRSVKGTLQNIREKITEHQIKNPAIIVIGDIVSFRQ
NSWFEEKPLIGLNIMAVKSCEEEQIPVEALREQGAEVVEWPKWKSAETVPDDALLGRIACFDSLLFTSSASLRRFFSRLA
ALKIDVREMRGRLYASDQHVKAALEERGFEAFLQTEMPVSGSCLLIGSREAVQNYPHSGCEVLITHDRVIDSRFTSIIKR
EIDEFPIDIVLFSNRTCVELFVNEAETVGLHPEKTAARLQAFCLTEEAGERAAAVGFQRVHAVNDRDRLPDVIKEINRTA
EFVCQS

Sequences:

>Translated_486_residues
MGNGKVYFVGAGPGDSGLITLKGRTLIEAADVILYDRLVNSRLLEHAKASCEFVYCGKLPDRHYMRQSEINALLIEKGLK
GLKVVRLKGGDPSVFGRVGEEAAAVSEHGIPYEMVPGITSGIAAPLYAGLPVTHRDFASSFAVVTAHDKSGKPEMNWEGL
AKGVQTLVFYMGIKNLGYICEQLMKHGKSPLVPAMAIQWGTWGRQRSVKGTLQNIREKITEHQIKNPAIIVIGDIVSFRQ
NSWFEEKPLIGLNIMAVKSCEEEQIPVEALREQGAEVVEWPKWKSAETVPDDALLGRIACFDSLLFTSSASLRRFFSRLA
ALKIDVREMRGRLYASDQHVKAALEERGFEAFLQTEMPVSGSCLLIGSREAVQNYPHSGCEVLITHDRVIDSRFTSIIKR
EIDEFPIDIVLFSNRTCVELFVNEAETVGLHPEKTAARLQAFCLTEEAGERAAAVGFQRVHAVNDRDRLPDVIKEINRTA
EFVCQS
>Mature_485_residues
GNGKVYFVGAGPGDSGLITLKGRTLIEAADVILYDRLVNSRLLEHAKASCEFVYCGKLPDRHYMRQSEINALLIEKGLKG
LKVVRLKGGDPSVFGRVGEEAAAVSEHGIPYEMVPGITSGIAAPLYAGLPVTHRDFASSFAVVTAHDKSGKPEMNWEGLA
KGVQTLVFYMGIKNLGYICEQLMKHGKSPLVPAMAIQWGTWGRQRSVKGTLQNIREKITEHQIKNPAIIVIGDIVSFRQN
SWFEEKPLIGLNIMAVKSCEEEQIPVEALREQGAEVVEWPKWKSAETVPDDALLGRIACFDSLLFTSSASLRRFFSRLAA
LKIDVREMRGRLYASDQHVKAALEERGFEAFLQTEMPVSGSCLLIGSREAVQNYPHSGCEVLITHDRVIDSRFTSIIKRE
IDEFPIDIVLFSNRTCVELFVNEAETVGLHPEKTAARLQAFCLTEEAGERAAAVGFQRVHAVNDRDRLPDVIKEINRTAE
FVCQS

Specific function: Multifunctional Enzyme That Catalyze The Sam-Dependent Methylation Of Uroporphyrinogen III At Position C-2 And C-7 To Form Precorrin-2 And Then Position C-12 Or C-18 To Form Trimethylpyrrocorphin 2. It Also Catalyzes The Conversion Of Precorrin-2 Into Sir

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=43.801652892562, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322922, Length=250, Percent_Identity=32, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 53873; Mature: 53741

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGKVYFVGAGPGDSGLITLKGRTLIEAADVILYDRLVNSRLLEHAKASCEFVYCGKLP
CCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DRHYMRQSEINALLIEKGLKGLKVVRLKGGDPSVFGRVGEEAAAVSEHGIPYEMVPGITS
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHCCCCHHHCCCCHH
GIAAPLYAGLPVTHRDFASSFAVVTAHDKSGKPEMNWEGLAKGVQTLVFYMGIKNLGYIC
HHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQLMKHGKSPLVPAMAIQWGTWGRQRSVKGTLQNIREKITEHQIKNPAIIVIGDIVSFRQ
HHHHHCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHC
NSWFEEKPLIGLNIMAVKSCEEEQIPVEALREQGAEVVEWPKWKSAETVPDDALLGRIAC
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHH
FDSLLFTSSASLRRFFSRLAALKIDVREMRGRLYASDQHVKAALEERGFEAFLQTEMPVS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCC
GSCLLIGSREAVQNYPHSGCEVLITHDRVIDSRFTSIIKREIDEFPIDIVLFSNRTCVEL
CCEEEEECHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
FVNEAETVGLHPEKTAARLQAFCLTEEAGERAAAVGFQRVHAVNDRDRLPDVIKEINRTA
EECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EFVCQS
HHHCCC
>Mature Secondary Structure 
GNGKVYFVGAGPGDSGLITLKGRTLIEAADVILYDRLVNSRLLEHAKASCEFVYCGKLP
CCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DRHYMRQSEINALLIEKGLKGLKVVRLKGGDPSVFGRVGEEAAAVSEHGIPYEMVPGITS
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHCCCCHHHCCCCHH
GIAAPLYAGLPVTHRDFASSFAVVTAHDKSGKPEMNWEGLAKGVQTLVFYMGIKNLGYIC
HHHHHHHCCCCCCHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQLMKHGKSPLVPAMAIQWGTWGRQRSVKGTLQNIREKITEHQIKNPAIIVIGDIVSFRQ
HHHHHCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHC
NSWFEEKPLIGLNIMAVKSCEEEQIPVEALREQGAEVVEWPKWKSAETVPDDALLGRIAC
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHH
FDSLLFTSSASLRRFFSRLAALKIDVREMRGRLYASDQHVKAALEERGFEAFLQTEMPVS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCC
GSCLLIGSREAVQNYPHSGCEVLITHDRVIDSRFTSIIKREIDEFPIDIVLFSNRTCVEL
CCEEEEECHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEE
FVNEAETVGLHPEKTAARLQAFCLTEEAGERAAAVGFQRVHAVNDRDRLPDVIKEINRTA
EECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EFVCQS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]