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The map label for this gene is yclK [H]

Identifier: 52784280

GI number: 52784280

Start: 461101

End: 462528

Strand: Direct

Name: yclK [H]

Synonym: BLi00460

Alternate gene names: 52784280

Gene position: 461101-462528 (Clockwise)

Preceding gene: 52784279

Following gene: 52784283

Centisome position: 10.92

GC content: 48.11

Gene sequence:

>1428_bases
ATGAAGATTAAGTATCTCTATCAGCTGCTGGCCAGTCACATCAGTATATTAGTCTTGGCTTTTCTCATCACGGGGGCGCT
GTTTTCACACTTTGCCAAAGACTTTGCATACGAGAGCAAAGTCGATGAGCTGTCTTCCTACGGCTACCGCATCATGGGCG
AAATGGAGATGCGGCCGAAAGGAGCGCCGGCCATTATGAAGCCGTTTGAGGAAATTTTGCGAAGCAGGAAGATCAGCTTT
TTTCTGTTTAATGAAAAGAAAGAGATCTTGATGACGCCGGCCGATTTGCGGCCGCGAAAACTGCTGCTCAGTGATGAAGA
ATGGAAAGAGCTTAAAGATGGAAAGGCCGTTGTCGTCAAAACCGACTTTAACCGTTTTAATGAGGAAGTATCGGTCGTCG
CTCTGCCCAACATCGTCAACAATGAATTTCAGGGAGGCATTCTGCTGACCGCTCCGATCAGCGGGACCGAAGAAATGATC
AGCGACATGAACCGCTTTATGCTCTTTACGGCGGCGGGTGTCATCTTAGTCGCTATTCTCTTAAGCTGGCTGACGTCGAA
ATTCCATGTCGCCCGCATTCAGAAGCTCCGCGACGCCACGAAAAAAGTCGCGGCCGGGGATTATGACATCCATATCGAAA
ACCGCAATAAGGATGAAATCGGTGATTTGGGCGACGACTTTAACATTATGGCCCGCAAGCTAAAAAGCTCAAGCGAGGAA
ATCGACCGGCTCGAAAAGCGGAGAAGGCAGTTTATCACTGACGTTTCACATGAGCTGAAGACGCCGCTGACGACGATCAG
CGGTTTAGTGGAAGGGATGAACAGCCAGACGATACCCGAAGACCAGCAGGATAAATGTATGAAGCTGATCACGGAGGAGA
CAAAAAGGCTGATTCGCCTCGTCAACGAAAACCTTGATTATGAAAAAATCCGCTCCAACCAGATTACATTGCAAAAGCTG
CATTATCCGCTGATCGAAGTGTTTGAAATCATTAAAGAGCAGCTGGCGCTTCAGGCGGAGGAGAGACAGAATCAGCTGAT
CCTCGATGTGGAAGATCATGTAATGGTCAATGCCGATTACGACCGGCTCATCCAGATTCTCGTCAATATTACGAAGAACA
GCATTCAATTCACAACGAACGGAACCGTTTGGCTGAGAGGCCGGGAAGACTACAAGGAGACGGTGATCGAAGTAGAGGAT
ACCGGAATCGGCATTGATGAAAATGATATCGAAAACATTTGGGATCGTTTTTATAAAGCGGATTTATCACGGACAAACAC
ACCTTACGGAGAATACGGGCTGGGGCTGTCGATCGTCAGACAGCTCGTCTCCCTTCACAACGGCACGGTAGAGATTGAAA
GCGAGAAAAACAAAGGAACAAAATTTATCATCCGCCTGCCGCTGACGGCGAGAGATGATGATGAATAG

Upstream 100 bases:

>100_bases
GAGCGGACTGTGGATGTTCATATTAAACGGCTGAGAAAAAAGCTTGGCAGCGACTCGCGGCCGTTTCTTTACACCGTATG
GGGAGTAGGGTACAAGTTCG

Downstream 100 bases:

>100_bases
AAACCCGCCAGACAGCGTCTGGCGGGTTTTTGTTAATCTTTAATTTTGAATTTCTGAATCAGGTGCTGCAGCTCTTCGGC
GTTTTGCGCGAGGATCTGTG

Product: YclK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKIKYLYQLLASHISILVLAFLITGALFSHFAKDFAYESKVDELSSYGYRIMGEMEMRPKGAPAIMKPFEEILRSRKISF
FLFNEKKEILMTPADLRPRKLLLSDEEWKELKDGKAVVVKTDFNRFNEEVSVVALPNIVNNEFQGGILLTAPISGTEEMI
SDMNRFMLFTAAGVILVAILLSWLTSKFHVARIQKLRDATKKVAAGDYDIHIENRNKDEIGDLGDDFNIMARKLKSSSEE
IDRLEKRRRQFITDVSHELKTPLTTISGLVEGMNSQTIPEDQQDKCMKLITEETKRLIRLVNENLDYEKIRSNQITLQKL
HYPLIEVFEIIKEQLALQAEERQNQLILDVEDHVMVNADYDRLIQILVNITKNSIQFTTNGTVWLRGREDYKETVIEVED
TGIGIDENDIENIWDRFYKADLSRTNTPYGEYGLGLSIVRQLVSLHNGTVEIESEKNKGTKFIIRLPLTARDDDE

Sequences:

>Translated_475_residues
MKIKYLYQLLASHISILVLAFLITGALFSHFAKDFAYESKVDELSSYGYRIMGEMEMRPKGAPAIMKPFEEILRSRKISF
FLFNEKKEILMTPADLRPRKLLLSDEEWKELKDGKAVVVKTDFNRFNEEVSVVALPNIVNNEFQGGILLTAPISGTEEMI
SDMNRFMLFTAAGVILVAILLSWLTSKFHVARIQKLRDATKKVAAGDYDIHIENRNKDEIGDLGDDFNIMARKLKSSSEE
IDRLEKRRRQFITDVSHELKTPLTTISGLVEGMNSQTIPEDQQDKCMKLITEETKRLIRLVNENLDYEKIRSNQITLQKL
HYPLIEVFEIIKEQLALQAEERQNQLILDVEDHVMVNADYDRLIQILVNITKNSIQFTTNGTVWLRGREDYKETVIEVED
TGIGIDENDIENIWDRFYKADLSRTNTPYGEYGLGLSIVRQLVSLHNGTVEIESEKNKGTKFIIRLPLTARDDDE
>Mature_475_residues
MKIKYLYQLLASHISILVLAFLITGALFSHFAKDFAYESKVDELSSYGYRIMGEMEMRPKGAPAIMKPFEEILRSRKISF
FLFNEKKEILMTPADLRPRKLLLSDEEWKELKDGKAVVVKTDFNRFNEEVSVVALPNIVNNEFQGGILLTAPISGTEEMI
SDMNRFMLFTAAGVILVAILLSWLTSKFHVARIQKLRDATKKVAAGDYDIHIENRNKDEIGDLGDDFNIMARKLKSSSEE
IDRLEKRRRQFITDVSHELKTPLTTISGLVEGMNSQTIPEDQQDKCMKLITEETKRLIRLVNENLDYEKIRSNQITLQKL
HYPLIEVFEIIKEQLALQAEERQNQLILDVEDHVMVNADYDRLIQILVNITKNSIQFTTNGTVWLRGREDYKETVIEVED
TGIGIDENDIENIWDRFYKADLSRTNTPYGEYGLGLSIVRQLVSLHNGTVEIESEKNKGTKFIIRLPLTARDDDE

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=28.2229965156794, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=28.6307053941909, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=30.4166666666667, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789149, Length=336, Percent_Identity=25.8928571428571, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994928, Length=248, Percent_Identity=29.4354838709677, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786783, Length=308, Percent_Identity=27.5974025974026, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081816, Length=346, Percent_Identity=26.0115606936416, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=27.6785714285714, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790861, Length=396, Percent_Identity=23.989898989899, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788549, Length=285, Percent_Identity=24.9122807017544, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790346, Length=294, Percent_Identity=25.5102040816327, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=25.8474576271186, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=24.4, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787894, Length=317, Percent_Identity=25.2365930599369, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790551, Length=223, Percent_Identity=27.3542600896861, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=314, Percent_Identity=22.9299363057325, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=27.4678111587983, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787374, Length=285, Percent_Identity=20.3508771929825, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54706; Mature: 54706

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKYLYQLLASHISILVLAFLITGALFSHFAKDFAYESKVDELSSYGYRIMGEMEMRPK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
GAPAIMKPFEEILRSRKISFFLFNEKKEILMTPADLRPRKLLLSDEEWKELKDGKAVVVK
CCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCEEECCCHHHHHHCCCCEEEEE
TDFNRFNEEVSVVALPNIVNNEFQGGILLTAPISGTEEMISDMNRFMLFTAAGVILVAIL
ECCHHCCCCEEEEECCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSWLTSKFHVARIQKLRDATKKVAAGDYDIHIENRNKDEIGDLGDDFNIMARKLKSSSEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHCCHHH
IDRLEKRRRQFITDVSHELKTPLTTISGLVEGMNSQTIPEDQQDKCMKLITEETKRLIRL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VNENLDYEKIRSNQITLQKLHYPLIEVFEIIKEQLALQAEERQNQLILDVEDHVMVNADY
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCH
DRLIQILVNITKNSIQFTTNGTVWLRGREDYKETVIEVEDTGIGIDENDIENIWDRFYKA
HHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHH
DLSRTNTPYGEYGLGLSIVRQLVSLHNGTVEIESEKNKGTKFIIRLPLTARDDDE
HHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MKIKYLYQLLASHISILVLAFLITGALFSHFAKDFAYESKVDELSSYGYRIMGEMEMRPK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
GAPAIMKPFEEILRSRKISFFLFNEKKEILMTPADLRPRKLLLSDEEWKELKDGKAVVVK
CCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCEEECCCHHHHHHCCCCEEEEE
TDFNRFNEEVSVVALPNIVNNEFQGGILLTAPISGTEEMISDMNRFMLFTAAGVILVAIL
ECCHHCCCCEEEEECCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSWLTSKFHVARIQKLRDATKKVAAGDYDIHIENRNKDEIGDLGDDFNIMARKLKSSSEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHCCHHH
IDRLEKRRRQFITDVSHELKTPLTTISGLVEGMNSQTIPEDQQDKCMKLITEETKRLIRL
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VNENLDYEKIRSNQITLQKLHYPLIEVFEIIKEQLALQAEERQNQLILDVEDHVMVNADY
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCH
DRLIQILVNITKNSIQFTTNGTVWLRGREDYKETVIEVEDTGIGIDENDIENIWDRFYKA
HHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHH
DLSRTNTPYGEYGLGLSIVRQLVSLHNGTVEIESEKNKGTKFIIRLPLTARDDDE
HHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]