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The map label for this gene is nylA [H]

Identifier: 52784148

GI number: 52784148

Start: 298777

End: 300276

Strand: Direct

Name: nylA [H]

Synonym: BLi00325

Alternate gene names: 52784148

Gene position: 298777-300276 (Clockwise)

Preceding gene: 52784146

Following gene: 52784151

Centisome position: 7.08

GC content: 51.0

Gene sequence:

>1500_bases
ATGAATATATCTGACTATGTAAAGCATGACGCGGTTGCTTTATCTAAGCTTATCAAACATGGGGAAGTAACGGTCAACGA
ATTAATCGACGCTGCTTTTTCCCGTCTTGAAGAAGTCAATCCAGAACTGAATGCAGTCATTCGGACAAGAAAAGAGCAGG
TCAAAATTGAATCCAAACAGCTTGACATCAGACACCAGCCGTTTGCCGGGGTGCCGATTCTTTTAAAGGACATTTCCCAA
GCGTTGAAAGGGGAGCCTCTCACAGCCGGCGCCAAGCTGCTTTCATCTTATAAACCAAAGCGCGATTCGAATTTCGTCGC
GAAATTGCGCGGCGCCGGTTTTTTATTTCTCGGACAGACGAATACACCCGAATACGGATTAAAAAATATTTCTGAGCCTG
AATTGTATGGGCCGTCGCGAAATCCTTGGAACACTGATTATTCACCCGGGGGTTCAAGCGGCGGTTCTGCGGCTGCGGTG
GCGGCCGGAATCGTTCCACTTGCAGGGGCAAGCGACGGGGGAGGCTCCATCCGGATACCGGCTTCCTTTACCGGACTATT
CGGTCTGAAACCGACCCGGGGCAGAACGCCTGTAGGACCGGGCGCCGGACGGCAGTGGCAGGGGGCATCGATCGATTTTG
TGCTGTCCCGTACGGTCAGGGACAGCGCGGCGCTCCTGGATATTCTGCAAACCATTCAGCCTGAAGCCGCTTTTCAAACG
CCTTTATTTCCAGGTCAGTATGAAGAGGATATGAAAAAAGGGCTGGAGCGCCCTTTGAAAATCGCATTCTCTGTAGAATC
ACCCGTCGGAACGCCTGTCAGCGATGAAGCCAAACAAGCCGTCCGCCGCACCGTTCGATGGCTGGAAGCGCAGGGACACC
ATGTCGAAGAGCAGACGAACGGCGTTGACGGAATCCGGTTAATGGAGAACTACTACTTAATGAACAGCGGTGAAATTTCC
GCGATGATTCTTGGAATGGAAAAAACGCTTGGAAGAGCGATCACTGTTCAGGACGTTGAAATTGTCACATGGGTGCTGAA
TGAAGCAGGCAAAAAAGTGTCGGCAGCCGAATTTACAGCAAGCCTTGCTTCCTGGGATCGAGCAGCTGCACAAATGGCGG
CGTTTCATGAGACCTATGATCTGTATGTGACCCCGGCAACGGCTTACCCCGCTCCAAAGGTCGGCGAGCTCACCCATACC
GAAAAAGAAGCCGAAGCGCTCCTGAAAGTAAGTGAGCTGGAAAAAATCGAGCAGCAGTCGCTGATTTATCAAATGTTTTT
GAAAAGCTTGACATATACACCATTCACACAGCTTGCCAACTTAACCGGACAGCCTGCAATGAGCGTCCCGGTCCATCTGA
CAAATGAAGGTTTGCCGCTGGGTGTTCAAGTGACGGCTCCGAAAGGAAAGGAACATTGGCTGCTGCGCACCGCCCATTTA
TTAGAGCAATCAGACTTATGGATCGGAATGAAAGGAAATCCAATGCTCGGCAGTCTGTAA

Upstream 100 bases:

>100_bases
TTTCACTCATCTCCTTTTGATCTAAACAGAAATCGTTGAAATCCCCAAAAGGCTCATGCACAATAGTAAGCACAAGCGAA
CAGTAAAGTGAGGTACTGAT

Downstream 100 bases:

>100_bases
CCGTATAAGAGAGGACCGCCCGTTAATCAGGCCGGTCTTCTTTTTTATCGTACAGTTTTTCAAGCTCGTCAACCAATGTT
CCGACATACGTCACAGCCCG

Product: hypothetical protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQLDIRHQPFAGVPILLKDISQ
ALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLGQTNTPEYGLKNISEPELYGPSRNPWNTDYSPGGSSGGSAAAV
AAGIVPLAGASDGGGSIRIPASFTGLFGLKPTRGRTPVGPGAGRQWQGASIDFVLSRTVRDSAALLDILQTIQPEAAFQT
PLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRTVRWLEAQGHHVEEQTNGVDGIRLMENYYLMNSGEIS
AMILGMEKTLGRAITVQDVEIVTWVLNEAGKKVSAAEFTASLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHT
EKEAEALLKVSELEKIEQQSLIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQVTAPKGKEHWLLRTAHL
LEQSDLWIGMKGNPMLGSL

Sequences:

>Translated_499_residues
MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQLDIRHQPFAGVPILLKDISQ
ALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLGQTNTPEYGLKNISEPELYGPSRNPWNTDYSPGGSSGGSAAAV
AAGIVPLAGASDGGGSIRIPASFTGLFGLKPTRGRTPVGPGAGRQWQGASIDFVLSRTVRDSAALLDILQTIQPEAAFQT
PLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRTVRWLEAQGHHVEEQTNGVDGIRLMENYYLMNSGEIS
AMILGMEKTLGRAITVQDVEIVTWVLNEAGKKVSAAEFTASLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHT
EKEAEALLKVSELEKIEQQSLIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQVTAPKGKEHWLLRTAHL
LEQSDLWIGMKGNPMLGSL
>Mature_499_residues
MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQLDIRHQPFAGVPILLKDISQ
ALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLGQTNTPEYGLKNISEPELYGPSRNPWNTDYSPGGSSGGSAAAV
AAGIVPLAGASDGGGSIRIPASFTGLFGLKPTRGRTPVGPGAGRQWQGASIDFVLSRTVRDSAALLDILQTIQPEAAFQT
PLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRTVRWLEAQGHHVEEQTNGVDGIRLMENYYLMNSGEIS
AMILGMEKTLGRAITVQDVEIVTWVLNEAGKKVSAAEFTASLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHT
EKEAEALLKVSELEKIEQQSLIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQVTAPKGKEHWLLRTAHL
LEQSDLWIGMKGNPMLGSL

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=197, Percent_Identity=35.5329949238579, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=23.7354085603113, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=24.2063492063492, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=527, Percent_Identity=24.2884250474383, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=28.8461538461538, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=30.9446254071661, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=30.9446254071661, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=135, Percent_Identity=39.2592592592593, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=304, Percent_Identity=30.2631578947368, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=163, Percent_Identity=36.1963190184049, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=128, Percent_Identity=40.625, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=134, Percent_Identity=38.8059701492537, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=25.440313111546, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45550774, Length=195, Percent_Identity=34.3589743589744, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=195, Percent_Identity=34.3589743589744, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648437, Length=195, Percent_Identity=34.3589743589744, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356731, Length=516, Percent_Identity=24.031007751938, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652985, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922090, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652981, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24652983, Length=196, Percent_Identity=33.1632653061224, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648441, Length=439, Percent_Identity=23.6902050113895, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648439, Length=439, Percent_Identity=23.6902050113895, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=26.1224489795918, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI161078093, Length=200, Percent_Identity=31.5, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 54197; Mature: 54197

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQ
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEHHHH
LDIRHQPFAGVPILLKDISQALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLGQT
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECC
NTPEYGLKNISEPELYGPSRNPWNTDYSPGGSSGGSAAAVAAGIVPLAGASDGGGSIRIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEEEC
ASFTGLFGLKPTRGRTPVGPGAGRQWQGASIDFVLSRTVRDSAALLDILQTIQPEAAFQT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHCC
PLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRTVRWLEAQGHHVEEQTN
CCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCC
GVDGIRLMENYYLMNSGEISAMILGMEKTLGRAITVQDVEIVTWVLNEAGKKVSAAEFTA
CCCCEEEEHHEEEECCCCEEEEEEEHHHHHCCEEEEEHHHHHHHHHHHCCCCCHHHHHHH
SLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHTEKEAEALLKVSELEKIEQQS
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQVTAPKGKEHWLLRTAHL
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHH
LEQSDLWIGMKGNPMLGSL
HCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQ
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEHHHH
LDIRHQPFAGVPILLKDISQALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLGQT
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEECC
NTPEYGLKNISEPELYGPSRNPWNTDYSPGGSSGGSAAAVAAGIVPLAGASDGGGSIRIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCCCCEEEEC
ASFTGLFGLKPTRGRTPVGPGAGRQWQGASIDFVLSRTVRDSAALLDILQTIQPEAAFQT
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHCC
PLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRTVRWLEAQGHHVEEQTN
CCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCC
GVDGIRLMENYYLMNSGEISAMILGMEKTLGRAITVQDVEIVTWVLNEAGKKVSAAEFTA
CCCCEEEEHHEEEECCCCEEEEEEEHHHHHCCEEEEEHHHHHHHHHHHCCCCCHHHHHHH
SLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHTEKEAEALLKVSELEKIEQQS
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQVTAPKGKEHWLLRTAHL
HHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHH
LEQSDLWIGMKGNPMLGSL
HCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]