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The map label for this gene is spaK [H]

Identifier: 52784139

GI number: 52784139

Start: 287862

End: 289232

Strand: Direct

Name: spaK [H]

Synonym: BLi00316

Alternate gene names: 52784139

Gene position: 287862-289232 (Clockwise)

Preceding gene: 52784138

Following gene: 52784141

Centisome position: 6.82

GC content: 43.18

Gene sequence:

>1371_bases
ATGGGAATAATCCGCAAAAAAAAGACACTGCGCAGAGAACTGATCAAATATATGCTCACTTTGAGCTTCAGCCTGATCGC
GATCACGGCTTTATATGTTTTGGTCAATATGATAGGAATGAATGCAGGGCTGTTTTATCCTGCCAACTACAATGAAATCG
AAGCTGAAAAGCTTAAGCCCAGGCTTCAATCGGCTGAACAGATAACGGAAGACATGATACCGGATACAATGCTGTACGCC
ATACTGGACAAAAAGTCGAAACAAAAGACAGCGGGAAACATGAAAGAGACGAATCTTTCGATTGTACGGAAAAAGATAGA
GAGCAGGCCTTATGTCAGCTTTAGTCAAAAAGGCTATATGATTATCGAACGCAAAGACGAGTATTGTGTATTGGAGTATC
CTTTGCGCGCTGAGTTTCGGTCTCCACTGCTGAGACGGTTTCTCCCGAACTATGAGCTGACAAGCATATGCATCATCGTT
CTTCTTTTAATTACGGTCGTTTTTACGGTGACAACCCGATTTGCAAACAGATTAAAAGAACATTTTCAAACTTTAAACAC
CATTGCCGAGCAAATTAAAAAACAGAATTTAAATTTTACTCCGGAGTTCTCGAATATTAAGGAATTTGATCATGTAATCA
GTTCCCTGATGGACATGAGGAATGCCCTCCAAGCCTCCCTACGGGCGCAGTGGCATTTAGAGAAAACGAAAAAAGAACAG
ATCGGCGCATTGGCTCATGATATTAAAATTCCAGTGACCATCATCAAAGGAAATGCGGAGTTGCTGAGCTTATCTCCGCA
AAATCAAGAGCAGTCGGAATATATCAGATACATCCTTGACGCGGGGCAAAAAATCGAACAGTACATTGACCAATTAATCC
ATCTCTCAAAAACTGAGGAAGCATTACATACCGAGTACAGAGAAGGCGCTGTGAAAACGCTAGTCGAAAGTGTTCTCCAT
GAGACTGAAGCATATATAGGGAACAAATCGATTGACATTGTGTTGAAGGAGCAGAACCTTGAAGGAAAAAAAGCAGTGTT
TGACAAACAGCTGCTTCACCGAGCGTTGATGAACATCATGACGAACGCCGCAGATCACACACCAGAAGGCGGGAGAATCG
ATTTTGAAGCTGTGTGTACGGCTGATCAATTGATTTTCACAATCACTGACAGCGGAAAAGGGTTTTCTCAAGACGGCCTG
CAGAAAGCTGCCGAGCTTTTCTATATGGAAGATAAAGCCCGGACATCGAATGGTCATTACGGGATGGGACTTACCTTTGC
ACTTCGTGTTGTTAAGCTTCATCAAGGCGATTTGGCGATTAAAAACAGCGAAGCAGGAGGCGGACAGGTACAAGTGACCA
TCCCCCTCTGA

Upstream 100 bases:

>100_bases
CTTTATATTCGACGATAACCGAATTTATCCGCACGATTCGGAAAAAATGCAAAGAACACAATAGAGATCCGATCAAAACG
GTATGGGGCGTAGGATACAG

Downstream 100 bases:

>100_bases
GACCAGATCATCATTTCCATATCTCGTCATTCTCATAATCAAAAACCTTCCGGCATCGGCCGGGAAGGTTTTTTACGGTT
CTCCAAGTTCCTGTTTCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MGIIRKKKTLRRELIKYMLTLSFSLIAITALYVLVNMIGMNAGLFYPANYNEIEAEKLKPRLQSAEQITEDMIPDTMLYA
ILDKKSKQKTAGNMKETNLSIVRKKIESRPYVSFSQKGYMIIERKDEYCVLEYPLRAEFRSPLLRRFLPNYELTSICIIV
LLLITVVFTVTTRFANRLKEHFQTLNTIAEQIKKQNLNFTPEFSNIKEFDHVISSLMDMRNALQASLRAQWHLEKTKKEQ
IGALAHDIKIPVTIIKGNAELLSLSPQNQEQSEYIRYILDAGQKIEQYIDQLIHLSKTEEALHTEYREGAVKTLVESVLH
ETEAYIGNKSIDIVLKEQNLEGKKAVFDKQLLHRALMNIMTNAADHTPEGGRIDFEAVCTADQLIFTITDSGKGFSQDGL
QKAAELFYMEDKARTSNGHYGMGLTFALRVVKLHQGDLAIKNSEAGGGQVQVTIPL

Sequences:

>Translated_456_residues
MGIIRKKKTLRRELIKYMLTLSFSLIAITALYVLVNMIGMNAGLFYPANYNEIEAEKLKPRLQSAEQITEDMIPDTMLYA
ILDKKSKQKTAGNMKETNLSIVRKKIESRPYVSFSQKGYMIIERKDEYCVLEYPLRAEFRSPLLRRFLPNYELTSICIIV
LLLITVVFTVTTRFANRLKEHFQTLNTIAEQIKKQNLNFTPEFSNIKEFDHVISSLMDMRNALQASLRAQWHLEKTKKEQ
IGALAHDIKIPVTIIKGNAELLSLSPQNQEQSEYIRYILDAGQKIEQYIDQLIHLSKTEEALHTEYREGAVKTLVESVLH
ETEAYIGNKSIDIVLKEQNLEGKKAVFDKQLLHRALMNIMTNAADHTPEGGRIDFEAVCTADQLIFTITDSGKGFSQDGL
QKAAELFYMEDKARTSNGHYGMGLTFALRVVKLHQGDLAIKNSEAGGGQVQVTIPL
>Mature_455_residues
GIIRKKKTLRRELIKYMLTLSFSLIAITALYVLVNMIGMNAGLFYPANYNEIEAEKLKPRLQSAEQITEDMIPDTMLYAI
LDKKSKQKTAGNMKETNLSIVRKKIESRPYVSFSQKGYMIIERKDEYCVLEYPLRAEFRSPLLRRFLPNYELTSICIIVL
LLITVVFTVTTRFANRLKEHFQTLNTIAEQIKKQNLNFTPEFSNIKEFDHVISSLMDMRNALQASLRAQWHLEKTKKEQI
GALAHDIKIPVTIIKGNAELLSLSPQNQEQSEYIRYILDAGQKIEQYIDQLIHLSKTEEALHTEYREGAVKTLVESVLHE
TEAYIGNKSIDIVLKEQNLEGKKAVFDKQLLHRALMNIMTNAADHTPEGGRIDFEAVCTADQLIFTITDSGKGFSQDGLQ
KAAELFYMEDKARTSNGHYGMGLTFALRVVKLHQGDLAIKNSEAGGGQVQVTIPL

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=329, Percent_Identity=25.8358662613982, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=24.8888888888889, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790861, Length=238, Percent_Identity=26.4705882352941, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081816, Length=336, Percent_Identity=23.2142857142857, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=24.0174672489083, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=355, Percent_Identity=22.2535211267606, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=23.8938053097345, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51929; Mature: 51798

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIIRKKKTLRRELIKYMLTLSFSLIAITALYVLVNMIGMNAGLFYPANYNEIEAEKLKP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHH
RLQSAEQITEDMIPDTMLYAILDKKSKQKTAGNMKETNLSIVRKKIESRPYVSFSQKGYM
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCEE
IIERKDEYCVLEYPLRAEFRSPLLRRFLPNYELTSICIIVLLLITVVFTVTTRFANRLKE
EEEECCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFQTLNTIAEQIKKQNLNFTPEFSNIKEFDHVISSLMDMRNALQASLRAQWHLEKTKKEQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGALAHDIKIPVTIIKGNAELLSLSPQNQEQSEYIRYILDAGQKIEQYIDQLIHLSKTEE
HHHHHHCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ALHTEYREGAVKTLVESVLHETEAYIGNKSIDIVLKEQNLEGKKAVFDKQLLHRALMNIM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TNAADHTPEGGRIDFEAVCTADQLIFTITDSGKGFSQDGLQKAAELFYMEDKARTSNGHY
HCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GMGLTFALRVVKLHQGDLAIKNSEAGGGQVQVTIPL
CCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECC
>Mature Secondary Structure 
GIIRKKKTLRRELIKYMLTLSFSLIAITALYVLVNMIGMNAGLFYPANYNEIEAEKLKP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHH
RLQSAEQITEDMIPDTMLYAILDKKSKQKTAGNMKETNLSIVRKKIESRPYVSFSQKGYM
HHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCEE
IIERKDEYCVLEYPLRAEFRSPLLRRFLPNYELTSICIIVLLLITVVFTVTTRFANRLKE
EEEECCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFQTLNTIAEQIKKQNLNFTPEFSNIKEFDHVISSLMDMRNALQASLRAQWHLEKTKKEQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGALAHDIKIPVTIIKGNAELLSLSPQNQEQSEYIRYILDAGQKIEQYIDQLIHLSKTEE
HHHHHHCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ALHTEYREGAVKTLVESVLHETEAYIGNKSIDIVLKEQNLEGKKAVFDKQLLHRALMNIM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
TNAADHTPEGGRIDFEAVCTADQLIFTITDSGKGFSQDGLQKAAELFYMEDKARTSNGHY
HCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GMGLTFALRVVKLHQGDLAIKNSEAGGGQVQVTIPL
CCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]