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The map label for this gene is spaR [H]

Identifier: 52784138

GI number: 52784138

Start: 287209

End: 287871

Strand: Direct

Name: spaR [H]

Synonym: BLi00315

Alternate gene names: 52784138

Gene position: 287209-287871 (Clockwise)

Preceding gene: 52784137

Following gene: 52784139

Centisome position: 6.8

GC content: 41.18

Gene sequence:

>663_bases
GTGGCGAAAATTTTGGCAGTAGATGATGAGAAAGATATTCTTGTACTCATACAAAATATTTTGCGCCGCGATCAGCATCA
AGTTCATACGCTAAGTCAAGTTAACACCGAATCTCTCGACGTGTTTTCAGGGTATGATTTAATTTTGCTTGATGTGATGA
TGCCGGGGATGGACGGGTTTGAATTATGCGAGAAAATCCGGCCGCTGGTAGATTGTCCGATCCTGTTTCTCACCGCTAAG
ACGGAGGAAGAGGCCATCGTTAAAGGCCTCCTGACTGGGGGAGACGACTATATTTCAAAACCCTTCGGCGTTCTTGAGCT
CAGTGCAAGAGTAAATGCACACCTGAGAAGGGAAAGAAGAGAGAAATACGAGACTAAACGGGTGATTTCCGGTTTTTTAT
TTCACTTTGACAGCAAAGAAGTATTTATTCATGATCAAAAGCTGAATTTAACAAAAAATGAATTTAAAATATGTGAGCTA
TTGGCTCAGCATAAAGGACGAACCTTTACAAGAGAAGAAATTTATGATCATGTATACGGGCTGGAAGGCAATGCTTTATA
TTCGACGATAACCGAATTTATCCGCACGATTCGGAAAAAATGCAAAGAACACAATAGAGATCCGATCAAAACGGTATGGG
GCGTAGGATACAGATGGGAATAA

Upstream 100 bases:

>100_bases
GGTGTTTGCTATAATAGCTGTATTCGCTCTAAGCATCATTTGGTTTATCCGCTGGGAAGGAAGAAAAGCTTTTGAATAAT
GAAAAAAGGGTGGTCAGCAA

Downstream 100 bases:

>100_bases
TCCGCAAAAAAAAGACACTGCGCAGAGAACTGATCAAATATATGCTCACTTTGAGCTTCAGCCTGATCGCGATCACGGCT
TTATATGTTTTGGTCAATAT

Product: hypothetical protein

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKILAVDDEKDILVLIQNILRRDQHQVHTLSQVNTESLDVFSGYDLILLDVMMPGMDGFELCEKIRPLVDCPILFLTAK
TEEEAIVKGLLTGGDDYISKPFGVLELSARVNAHLRRERREKYETKRVISGFLFHFDSKEVFIHDQKLNLTKNEFKICEL
LAQHKGRTFTREEIYDHVYGLEGNALYSTITEFIRTIRKKCKEHNRDPIKTVWGVGYRWE

Sequences:

>Translated_220_residues
MAKILAVDDEKDILVLIQNILRRDQHQVHTLSQVNTESLDVFSGYDLILLDVMMPGMDGFELCEKIRPLVDCPILFLTAK
TEEEAIVKGLLTGGDDYISKPFGVLELSARVNAHLRRERREKYETKRVISGFLFHFDSKEVFIHDQKLNLTKNEFKICEL
LAQHKGRTFTREEIYDHVYGLEGNALYSTITEFIRTIRKKCKEHNRDPIKTVWGVGYRWE
>Mature_219_residues
AKILAVDDEKDILVLIQNILRRDQHQVHTLSQVNTESLDVFSGYDLILLDVMMPGMDGFELCEKIRPLVDCPILFLTAKT
EEEAIVKGLLTGGDDYISKPFGVLELSARVNAHLRRERREKYETKRVISGFLFHFDSKEVFIHDQKLNLTKNEFKICELL
AQHKGRTFTREEIYDHVYGLEGNALYSTITEFIRTIRKKCKEHNRDPIKTVWGVGYRWE

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=33.4801762114537, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=34.375, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=32.4324324324324, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1790860, Length=176, Percent_Identity=35.2272727272727, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082012, Length=178, Percent_Identity=34.2696629213483, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786784, Length=190, Percent_Identity=33.1578947368421, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=27.3542600896861, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=31.858407079646, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1789402, Length=217, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1787375, Length=179, Percent_Identity=31.8435754189944, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=27.9475982532751, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=25.5230125523013, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25631; Mature: 25500

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILAVDDEKDILVLIQNILRRDQHQVHTLSQVNTESLDVFSGYDLILLDVMMPGMDGF
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCHH
ELCEKIRPLVDCPILFLTAKTEEEAIVKGLLTGGDDYISKPFGVLELSARVNAHLRRERR
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHH
EKYETKRVISGFLFHFDSKEVFIHDQKLNLTKNEFKICELLAQHKGRTFTREEIYDHVYG
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
LEGNALYSTITEFIRTIRKKCKEHNRDPIKTVWGVGYRWE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
AKILAVDDEKDILVLIQNILRRDQHQVHTLSQVNTESLDVFSGYDLILLDVMMPGMDGF
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCHH
ELCEKIRPLVDCPILFLTAKTEEEAIVKGLLTGGDDYISKPFGVLELSARVNAHLRRERR
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHHHHHHHHH
EKYETKRVISGFLFHFDSKEVFIHDQKLNLTKNEFKICELLAQHKGRTFTREEIYDHVYG
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
LEGNALYSTITEFIRTIRKKCKEHNRDPIKTVWGVGYRWE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]