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The map label for this gene is sigW [H]

Identifier: 52784027

GI number: 52784027

Start: 178118

End: 178681

Strand: Direct

Name: sigW [H]

Synonym: BLi00199

Alternate gene names: 52784027

Gene position: 178118-178681 (Clockwise)

Preceding gene: 52784026

Following gene: 52784028

Centisome position: 4.22

GC content: 42.91

Gene sequence:

>564_bases
ATGGAAACAATGATTAAAAAAAGAATCAAACAGGTGAAAAAAGGCGATCGTAACGCATTTGCAGACATCGTGGATATTTA
CAAAGACAAAATTTATCAGCTTTGCTACCGTATGCTCGGCAATGCGCATGAGGCGGAAGATATTGCGCAGGAAGCGTTTA
TCAGAGCATACGTGAATATCGACAGTTTTGATATCAACCGGAAATTTTCCACATGGCTTTATCGAATCGCAACCAATTTG
ACCATCGATCGTATCCGTAAAAAAAAGCCGGACTATTACTTGGATGCAGAGGTGGCAGGGACTGAAGGACTGACAATGTA
TTCCCAGGTTGCGGCGGATGTTGCGCTTCCAGAGGATGAAGTGGTTTCTTTGGAGCTGCACAGCACCATCCAGGAAAAAA
TCTTAAGGCTTCCCGATAAATACAGGACGGTCATCGTATTAAAGTACATTGATGAGCTTTCACTCATTGAGATCAGTGAA
ATCCTCAATATTCCCGTAGGTACGGTGAAAACGAGGATACACAGGGGCAGAGAGGCTCTCAGAAAACAATTAAGGGATCT
TTAA

Upstream 100 bases:

>100_bases
CCTGCCAAAAATTTGATAAACTATTTTTATGAATTTAATGAAACCTTATAAAAACCAATTCGTATACATAAAGACCGGTG
AAGGCAGAGGTTAGATAAAT

Downstream 100 bases:

>100_bases
GTGGGGTGATGAAATGAGCTGTCCGGAGCATATTGTTCAGCTTATGCATAAATACCTTGACGGAGACATACTTCCGGAAG
ATGAAACGCGTTTAAAAGAG

Product: RNA polymerase sigma factor SigW

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
METMIKKRIKQVKKGDRNAFADIVDIYKDKIYQLCYRMLGNAHEAEDIAQEAFIRAYVNIDSFDINRKFSTWLYRIATNL
TIDRIRKKKPDYYLDAEVAGTEGLTMYSQVAADVALPEDEVVSLELHSTIQEKILRLPDKYRTVIVLKYIDELSLIEISE
ILNIPVGTVKTRIHRGREALRKQLRDL

Sequences:

>Translated_187_residues
METMIKKRIKQVKKGDRNAFADIVDIYKDKIYQLCYRMLGNAHEAEDIAQEAFIRAYVNIDSFDINRKFSTWLYRIATNL
TIDRIRKKKPDYYLDAEVAGTEGLTMYSQVAADVALPEDEVVSLELHSTIQEKILRLPDKYRTVIVLKYIDELSLIEISE
ILNIPVGTVKTRIHRGREALRKQLRDL
>Mature_187_residues
METMIKKRIKQVKKGDRNAFADIVDIYKDKIYQLCYRMLGNAHEAEDIAQEAFIRAYVNIDSFDINRKFSTWLYRIATNL
TIDRIRKKKPDYYLDAEVAGTEGLTMYSQVAADVALPEDEVVSLELHSTIQEKILRLPDKYRTVIVLKYIDELSLIEISE
ILNIPVGTVKTRIHRGREALRKQLRDL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21795; Mature: 21795

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMIKKRIKQVKKGDRNAFADIVDIYKDKIYQLCYRMLGNAHEAEDIAQEAFIRAYVNI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
DSFDINRKFSTWLYRIATNLTIDRIRKKKPDYYLDAEVAGTEGLTMYSQVAADVALPEDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHCCCCCC
VVSLELHSTIQEKILRLPDKYRTVIVLKYIDELSLIEISEILNIPVGTVKTRIHRGREAL
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RKQLRDL
HHHHHCC
>Mature Secondary Structure
METMIKKRIKQVKKGDRNAFADIVDIYKDKIYQLCYRMLGNAHEAEDIAQEAFIRAYVNI
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
DSFDINRKFSTWLYRIATNLTIDRIRKKKPDYYLDAEVAGTEGLTMYSQVAADVALPEDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECCCCCHHHHHHHHHHHCCCCCC
VVSLELHSTIQEKILRLPDKYRTVIVLKYIDELSLIEISEILNIPVGTVKTRIHRGREAL
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RKQLRDL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]