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The map label for this gene is ybbF [H]

Identifier: 52784022

GI number: 52784022

Start: 172267

End: 173634

Strand: Reverse

Name: ybbF [H]

Synonym: BLi00189

Alternate gene names: 52784022

Gene position: 173634-172267 (Counterclockwise)

Preceding gene: 52784023

Following gene: 52784021

Centisome position: 4.11

GC content: 50.73

Gene sequence:

>1368_bases
ATGAGCGATGAGAAAAAATATGCCGGGCTGGCAAGACTGATTTTACAACACATCGGCGGCCCGTCTAACGTCGCAGATCA
TACCCACTGTATGACGCGCCTGCGCATCACGCCTGTCAACAGTCAAAAAACCGACATCGACTCGATCAAAGCGCTTGAAG
GCGTGATCGGCGTAGTTGAGGAGGAAACGCTTCAGATTATCCTCGGCACAGGCGTTGTACAGCACGTATCAGACGAGTTC
GGGAAGCTGCTTAAATCCGCAGAACACGTTGACTTAAAAGAATCCGCAGCCAAACATAAAGCGGAGATTTCCAAGAAAAA
CGCGACGCCTTTCAAGCTGTTTCTCCGGCGAATCGCAAGCATTTTTATACCGCTGATCCCCGCTTTGGTCGCCTCAGGCT
TGATTACCGGAATCACCAAAGCCGTTATCCAGGCTGGCTGGCTCGCGGAAACCTCACAAACCGCGATTATTTTAACGGTA
ATAGGCTCAGGTCTATTTACGTATTTGGGTATCCTGGTGGGGATCAATACGGCGAAAGAATTCGGCGGCTCCCCCGCACT
CGGCGGATTAGCCGGTATTTTAATCATTAACCCCGCCGTTGGTGAAATTTCACTTTTCGGTGAAGCGCTTTTGCCGGGAC
GCGGCGGTTTAATCGGCATTTTATTCGCAGCTGCATTTATCGCTTTTCTCGAAAAACGGGTCAGGCGCCTTGTACCTCAC
TCACTCGACATCATCATCACACCTACAGTCTCCCTTCTTATCACAGGAATCGTCACCTACGTTGTCTTCATGCCTGTCGG
CGGCTTGATTTCCGACGCCATTACGTCAGGTCTTCTCGCCATCTTAGATCTTGGGGGCATTATCGCCGGCTTTGTACTGG
GCGCCACCTTCCTTCCGCTCGTCGTAACCGGACTTCATCAAGGACTGACACCCGTTCATATGGAATTAATCAATTCAATC
GGCGATGACCCGCTGCTGCCGATTTTGGCCATGGGGGGCGCAGGACAAGTAGGCGCCGCTTTTGCGATCTATTTCAAAAC
GAAAAAAGCAAAGCTAAAAAGAGCCATTGCCGGCGGACTGCCGTCAGGCCTGCTCGGCATCGGCGAACCGCTCATATTCG
GTGTAACCCTTCCGCTCGGACGTCCGTTTTTAACCGCGTGCCTCGGGGCCGGAGTAGGCGGAGCATTTCAAGCGTATTTC
AAAATTGCGACCGTATCGATAGGCGTATCAGGGCTCCCTTTGTCGTTTTTGGTTCATACCCACCAAGTTCTTTTGTATAT
TCTCGGTTTGTTTATTTCCTATGCTGCCGGCTTTGTCTTAACATACTCCTTTGGTTTCAAGGACGATATGGCAGTTGAAT
TTGATTAA

Upstream 100 bases:

>100_bases
CCGCAGAGCGCTACGAGGACATCATTCAGTATATTGATCAAACAAAAGCGGCCATCCGCATGATGGGAATTTAGCGAATT
GTAAAAAAGGGGGATATAAA

Downstream 100 bases:

>100_bases
ACGTTTGTTTAAAATTTCCCACTATGCAATTTTTAACAGAAAGGAGTGAAGCAAGCTGAAAAAGTTTATGATCAGTGCGG
CCGCGTCCCTATTGCTGCTG

Product: YbbF

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSDEKKYAGLARLILQHIGGPSNVADHTHCMTRLRITPVNSQKTDIDSIKALEGVIGVVEEETLQIILGTGVVQHVSDEF
GKLLKSAEHVDLKESAAKHKAEISKKNATPFKLFLRRIASIFIPLIPALVASGLITGITKAVIQAGWLAETSQTAIILTV
IGSGLFTYLGILVGINTAKEFGGSPALGGLAGILIINPAVGEISLFGEALLPGRGGLIGILFAAAFIAFLEKRVRRLVPH
SLDIIITPTVSLLITGIVTYVVFMPVGGLISDAITSGLLAILDLGGIIAGFVLGATFLPLVVTGLHQGLTPVHMELINSI
GDDPLLPILAMGGAGQVGAAFAIYFKTKKAKLKRAIAGGLPSGLLGIGEPLIFGVTLPLGRPFLTACLGAGVGGAFQAYF
KIATVSIGVSGLPLSFLVHTHQVLLYILGLFISYAAGFVLTYSFGFKDDMAVEFD

Sequences:

>Translated_455_residues
MSDEKKYAGLARLILQHIGGPSNVADHTHCMTRLRITPVNSQKTDIDSIKALEGVIGVVEEETLQIILGTGVVQHVSDEF
GKLLKSAEHVDLKESAAKHKAEISKKNATPFKLFLRRIASIFIPLIPALVASGLITGITKAVIQAGWLAETSQTAIILTV
IGSGLFTYLGILVGINTAKEFGGSPALGGLAGILIINPAVGEISLFGEALLPGRGGLIGILFAAAFIAFLEKRVRRLVPH
SLDIIITPTVSLLITGIVTYVVFMPVGGLISDAITSGLLAILDLGGIIAGFVLGATFLPLVVTGLHQGLTPVHMELINSI
GDDPLLPILAMGGAGQVGAAFAIYFKTKKAKLKRAIAGGLPSGLLGIGEPLIFGVTLPLGRPFLTACLGAGVGGAFQAYF
KIATVSIGVSGLPLSFLVHTHQVLLYILGLFISYAAGFVLTYSFGFKDDMAVEFD
>Mature_454_residues
SDEKKYAGLARLILQHIGGPSNVADHTHCMTRLRITPVNSQKTDIDSIKALEGVIGVVEEETLQIILGTGVVQHVSDEFG
KLLKSAEHVDLKESAAKHKAEISKKNATPFKLFLRRIASIFIPLIPALVASGLITGITKAVIQAGWLAETSQTAIILTVI
GSGLFTYLGILVGINTAKEFGGSPALGGLAGILIINPAVGEISLFGEALLPGRGGLIGILFAAAFIAFLEKRVRRLVPHS
LDIIITPTVSLLITGIVTYVVFMPVGGLISDAITSGLLAILDLGGIIAGFVLGATFLPLVVTGLHQGLTPVHMELINSIG
DDPLLPILAMGGAGQVGAAFAIYFKTKKAKLKRAIAGGLPSGLLGIGEPLIFGVTLPLGRPFLTACLGAGVGGAFQAYFK
IATVSIGVSGLPLSFLVHTHQVLLYILGLFISYAAGFVLTYSFGFKDDMAVEFD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=466, Percent_Identity=37.3390557939914, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1790159, Length=419, Percent_Identity=29.1169451073986, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI2367362, Length=443, Percent_Identity=26.1851015801354, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI48994906, Length=472, Percent_Identity=27.1186440677966, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47560; Mature: 47429

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEKKYAGLARLILQHIGGPSNVADHTHCMTRLRITPVNSQKTDIDSIKALEGVIGVVE
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHH
EETLQIILGTGVVQHVSDEFGKLLKSAEHVDLKESAAKHKAEISKKNATPFKLFLRRIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IFIPLIPALVASGLITGITKAVIQAGWLAETSQTAIILTVIGSGLFTYLGILVGINTAKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHH
FGGSPALGGLAGILIINPAVGEISLFGEALLPGRGGLIGILFAAAFIAFLEKRVRRLVPH
CCCCCCHHHHEEHEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SLDIIITPTVSLLITGIVTYVVFMPVGGLISDAITSGLLAILDLGGIIAGFVLGATFLPL
CCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTGLHQGLTPVHMELINSIGDDPLLPILAMGGAGQVGAAFAIYFKTKKAKLKRAIAGGL
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCC
PSGLLGIGEPLIFGVTLPLGRPFLTACLGAGVGGAFQAYFKIATVSIGVSGLPLSFLVHT
CCCHHHCCCHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHH
HQVLLYILGLFISYAAGFVLTYSFGFKDDMAVEFD
HHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECC
>Mature Secondary Structure 
SDEKKYAGLARLILQHIGGPSNVADHTHCMTRLRITPVNSQKTDIDSIKALEGVIGVVE
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHH
EETLQIILGTGVVQHVSDEFGKLLKSAEHVDLKESAAKHKAEISKKNATPFKLFLRRIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IFIPLIPALVASGLITGITKAVIQAGWLAETSQTAIILTVIGSGLFTYLGILVGINTAKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHH
FGGSPALGGLAGILIINPAVGEISLFGEALLPGRGGLIGILFAAAFIAFLEKRVRRLVPH
CCCCCCHHHHEEHEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
SLDIIITPTVSLLITGIVTYVVFMPVGGLISDAITSGLLAILDLGGIIAGFVLGATFLPL
CCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTGLHQGLTPVHMELINSIGDDPLLPILAMGGAGQVGAAFAIYFKTKKAKLKRAIAGGL
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCC
PSGLLGIGEPLIFGVTLPLGRPFLTACLGAGVGGAFQAYFKIATVSIGVSGLPLSFLVHT
CCCHHHCCCHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHH
HQVLLYILGLFISYAAGFVLTYSFGFKDDMAVEFD
HHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]