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The map label for this gene is truA

Identifier: 52784004

GI number: 52784004

Start: 150869

End: 151612

Strand: Direct

Name: truA

Synonym: BLi00166

Alternate gene names: 52784004

Gene position: 150869-151612 (Clockwise)

Preceding gene: 52784003

Following gene: 52784005

Centisome position: 3.57

GC content: 47.98

Gene sequence:

>744_bases
ATGAGAATGAAGTGCACGATTTCATATGACGGACACCTTTTTTACGGCTACCAAGTGCAGCCTGGGCAAAGAACGATTCA
GGATGAGCTTGAAAAAGCGCTGCAAACGCTCCATAAAGCGAAGGAGCGTATTCCTGTCGTTTCATCCGGGCGCACCGACA
GCGGCGTCCACGCAGTCGGACAGACGATTCATTTTGACAGTCCGCTATCCATTCCTGAGGCGAAATGGCCGTATGCATTG
AATGCCCTGCTGCCCGATGATATATCGGTCCGCAAGGCGGAAGCTGTCAATGATCAATTTCATGCGAGGTTTTCTGCGAA
AAGAAAAGAATACCGGTATATGATATACAGGGGCAGACATCCGGACGTTTTTAAACGCTACTATGCATACCATGTTCCTT
ATGACCTTGATATGGAGAAAGTTAAGGAAGCCTCCCGCTATCTGGTCGGAACCCATGACTTCACAAGCTTTTGTGCGACC
AAAACCGAAGTGAAGGATAAAGTCAGAACGGTCCATGAACTGGAATGGTCTGACACAGGGGACGGACTGCAGATGAGAAT
TGTGGGAAGCGGATTTTTGTATAACATGGTTAGAATCATTGCCGGTACGCTTCTTGATGTAGGAACCGGCAAATTTTCCC
CTGGTGACATTGAAAAGATGATTCTTGCCAAAAACCGCGATGCCGCAGGGCGCACTGCACCGGCACACGGCTTATATTTA
TGGCGTGTTATCTATGACAACTAA

Upstream 100 bases:

>100_bases
TACAGGCAGCTGACATGGAAATGGAAGGATACTTCCGTGCTCCTCATTTTAGCTGTTCTTGGCGTTTTGCTAATTTTATT
GCGGACTTAGGTGATGGACG

Downstream 100 bases:

>100_bases
ACCAGGTGTAACATTTTCTTGACAGAGAGTACAGAAAGTTATAAGATATCATATGGTATGTATTTCAACCCCACGATAAG
CCCCGGAAACTTATTGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRMKCTISYDGHLFYGYQVQPGQRTIQDELEKALQTLHKAKERIPVVSSGRTDSGVHAVGQTIHFDSPLSIPEAKWPYAL
NALLPDDISVRKAEAVNDQFHARFSAKRKEYRYMIYRGRHPDVFKRYYAYHVPYDLDMEKVKEASRYLVGTHDFTSFCAT
KTEVKDKVRTVHELEWSDTGDGLQMRIVGSGFLYNMVRIIAGTLLDVGTGKFSPGDIEKMILAKNRDAAGRTAPAHGLYL
WRVIYDN

Sequences:

>Translated_247_residues
MRMKCTISYDGHLFYGYQVQPGQRTIQDELEKALQTLHKAKERIPVVSSGRTDSGVHAVGQTIHFDSPLSIPEAKWPYAL
NALLPDDISVRKAEAVNDQFHARFSAKRKEYRYMIYRGRHPDVFKRYYAYHVPYDLDMEKVKEASRYLVGTHDFTSFCAT
KTEVKDKVRTVHELEWSDTGDGLQMRIVGSGFLYNMVRIIAGTLLDVGTGKFSPGDIEKMILAKNRDAAGRTAPAHGLYL
WRVIYDN
>Mature_247_residues
MRMKCTISYDGHLFYGYQVQPGQRTIQDELEKALQTLHKAKERIPVVSSGRTDSGVHAVGQTIHFDSPLSIPEAKWPYAL
NALLPDDISVRKAEAVNDQFHARFSAKRKEYRYMIYRGRHPDVFKRYYAYHVPYDLDMEKVKEASRYLVGTHDFTSFCAT
KTEVKDKVRTVHELEWSDTGDGLQMRIVGSGFLYNMVRIIAGTLLDVGTGKFSPGDIEKMILAKNRDAAGRTAPAHGLYL
WRVIYDN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=251, Percent_Identity=32.2709163346614, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI199559785, Length=258, Percent_Identity=27.5193798449612, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=39.5918367346939, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71985046, Length=245, Percent_Identity=26.1224489795918, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI116007752, Length=298, Percent_Identity=27.8523489932886, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648455, Length=268, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362146, Length=268, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BACLD (Q65P74)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077433.1
- RefSeq:   YP_089833.1
- ProteinModelPortal:   Q65P74
- SMR:   Q65P74
- STRING:   Q65P74
- EnsemblBacteria:   EBBACT00000056003
- EnsemblBacteria:   EBBACT00000060733
- GeneID:   3029093
- GeneID:   3099352
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00166
- KEGG:   bli:BL01020
- NMPDR:   fig|279010.5.peg.1625
- eggNOG:   COG0101
- GeneTree:   EBGT00050000002107
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   BLIC279010-1:BLI00166-MONOMER
- BioCyc:   BLIC279010:BL01020-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28241; Mature: 28241

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKCTISYDGHLFYGYQVQPGQRTIQDELEKALQTLHKAKERIPVVSSGRTDSGVHAVG
CCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHC
QTIHFDSPLSIPEAKWPYALNALLPDDISVRKAEAVNDQFHARFSAKRKEYRYMIYRGRH
CEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCHHHHHHHHHHHHCEEEEEEECCC
PDVFKRYYAYHVPYDLDMEKVKEASRYLVGTHDFTSFCATKTEVKDKVRTVHELEWSDTG
CHHHHHHHHEECCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGLQMRIVGSGFLYNMVRIIAGTLLDVGTGKFSPGDIEKMILAKNRDAAGRTAPAHGLYL
CCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEE
WRVIYDN
EEEEECC
>Mature Secondary Structure
MRMKCTISYDGHLFYGYQVQPGQRTIQDELEKALQTLHKAKERIPVVSSGRTDSGVHAVG
CCEEEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHC
QTIHFDSPLSIPEAKWPYALNALLPDDISVRKAEAVNDQFHARFSAKRKEYRYMIYRGRH
CEEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCHHHHHHHHHHHHCEEEEEEECCC
PDVFKRYYAYHVPYDLDMEKVKEASRYLVGTHDFTSFCATKTEVKDKVRTVHELEWSDTG
CHHHHHHHHEECCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGLQMRIVGSGFLYNMVRIIAGTLLDVGTGKFSPGDIEKMILAKNRDAAGRTAPAHGLYL
CCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEE
WRVIYDN
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA